Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,568,519	C→A	A110A (GCG→GCT)	unknown ←	Phosphoglycerate transport system transcriptional regulatory protein PgtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,568,519	0	C	A	100.0%	81.2 / NA	30	A110A (GCG→GCT)	unknown	Phosphoglycerate transport system transcriptional regulatory protein PgtA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/7);  total (23/7)

TCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTCGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTG  >  1/1568291‑1568757                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               aagttctatatcactaagcaaaattaaacggaattgactctcgtcatgatgtaaaatctgaattaaatattgttgttcttcaacaggaagataatgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTg                                                                                                                                                                                                                           >  1:253250‑M2/150‑250 (MQ=255)      ctatatcactaagcaaaattaaacggaattgactctcgtcatgatgtaaaatctgaattaaatattgttgttcttcaacaggaagataatgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAAtc                                                                                                                                                                                                                     <  1:705590‑M2/107‑1 (MQ=255)                               gaattgactctcgtcatgatgtaaaatctgaattaaatattgttgttcttcaacaggaagataatgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAAc                                                                                                                                                                                                    <  1:149387‑M2/124‑1 (MQ=255)                                                                         ttgttcttcaacaggaagataatgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTaa                                                                                                                                                  >  1:437030‑M2/78‑251 (MQ=255)                                                                                      ggaagataatgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACActgc                                                                                                                                      <  1:152808‑M2/186‑1 (MQ=255)                                                                                          gataatgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAAtc                                                                                                                                                                                                                     <  1:543309‑M2/107‑1 (MQ=255)                                                                                           ataatgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCaa                                                                                                                                >  1:816438‑M2/60‑251 (MQ=255)                                                                                               tgcacattcttaataattaaagtccctgattttgtttctttaattagctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGtt                                                                                                                                                                 >  1:1020‑M2/56‑214 (MQ=255)                                                                                                                                             gctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTg                                                                                                                                                                   >  1:501848‑M2/10‑166 (MQ=255)                                                                                                                                             gctcaataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCAc                                                                               >  1:804040‑M2/10‑250 (MQ=255)                                                                                                                                                caataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGAt                                                                                                                                                                                            >  1:644087‑M2/7‑138 (MQ=255)                                                                                                                                                caataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATaaaaa                                                                                                                          >  1:321912‑M2/7‑204 (MQ=255)                                                                                                                                                caataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCttt                                                                           >  1:265934‑M2/7‑251 (MQ=255)                                                                                                                                                  ataaTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGgcgc                                                                                          <  1:549702‑M2/230‑1 (MQ=255)                                                                                                                                                     aTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTaa                                                                                                                                                                      >  1:736996‑M2/2‑155 (MQ=255)                                                                                                                                                     aTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCg                                                                      >  1:4846‑M2/2‑251 (MQ=255)                                                                                                                                                      tATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGt                                                                                                                       >  1:674002/1‑201 (MQ=255)                                                                                                                                                            tattatCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGttttttt                                                               >  1:486550/1‑251 (MQ=255)                                                                                                                                                              ttatCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTaa                                                             >  1:13341/1‑251 (MQ=255)                                                                                                                                                                atCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAg                                                           <  1:804716/251‑1 (MQ=255)                                                                                                                                                                        gtgtACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATg                                                                                                                                                                                                                                     >  1:653540/1‑73 (MQ=255)                                                                                                                                                                        gtgtACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCg                                                   >  1:684796/1‑251 (MQ=255)                                                                                                                                                                                tCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAAt                                           <  1:221647/251‑1 (MQ=255)                                                                                                                                                                                 cATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATa                                          >  1:811868/1‑251 (MQ=255)                                                                                                                                                                                                gTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAggg                           >  1:646543/1‑251 (MQ=255)                                                                                                                                                                                                               cGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCa            >  1:459518/1‑251 (MQ=255)                                                                                                                                                                                                               cGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCa            >  1:290842/1‑251 (MQ=255)                                                                                                                                                                                                                         aTGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTg  >  1:709721/1‑251 (MQ=255)                                                                                                                                                                                                                         aTGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTg  >  1:323353/1‑251 (MQ=255)                                                                                                                                                                                                                              gATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATcc                                                                                                                                               >  1:95139/1‑105 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               TCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCTATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTCGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCGTTTTTTTAAAGCGTTATCGACTTGAATAAGTAATTTTTCAGGGGAAACCGGTTTTTCAAAGAAATTTG  >  1/1568291‑1568757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑