Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	94,052	T→C	V269V (GTA→GTG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	94,052	0	T	C	100.0%	228.0 / NA	77	V269V (GTA→GTG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/25);  total (52/25)

TGCCGTACCGGCGTTAAGCTTAGTTAAAAAATAACCCTGATGCGCTGCCATCACTTACTCCACAAGCCGATAGGCGACACCATGGCGTCCGCTCGAATCCGGCAACTCTTTAACCCGCCAATCCAAATCGTTGTGATAGCCGCTCGCCGGGATAATTAACCATTTTTGAGCATTAATGGTTAAGATTGTGCGCGGGATGATGCCGTACATGTTTACATGATAACGGTCGGGTACAATGCCCAAG‑CGGGCGTAAGTATCATTGCGCAAATGCGCGGTGATGACGTTAGGATTGGGCATCACCACCTGCCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCGACAGAGCGGTCTAACATTGCCCCGGTATTAGCGCCGAAAAAATGCACTCCGGCAGTGGTATTATCAGATCTGTTGGCACCGCAAAAATACCACGGGCTGCGGCTTTCGC  >  1/93821‑94287                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             tGCCGTACCGGCGTTAAGCTTAGTTAAAAAATAACCCTGATGCGCTGCCATCACTTACTCCACAAGCCGATAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGt                                                                                                                                                                                                                          >  1:583020/1‑251 (MQ=255)        ccGGCGTTAAGCTTAGTTAAAAAATAACCCTGATGCGCTGCCATCACTTACTCCACAAGTCGATAGGCGCCACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATc                                                                                                                                                                                                                   <  1:388646/251‑1 (MQ=255)           gCGTTAAGCTTAGTTAAAAAATAACCCTGATGCGCTGCCATCACTTACTCCACAAGCCGATAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCAtt                                                                                                                                                                                                                >  1:13046/1‑251 (MQ=255)                                   cccTGATGCGCTGCCATCACTTACTCCACAAGCCGATAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGtt                                                                                                                                                                                        >  1:656424/1‑251 (MQ=255)                                             cccccaccccttcccccaaaaGCCGATAGGCGACCCCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGc                                                                                                                                                                              <  1:587797/232‑1 (MQ=255)                                             cccccaccccttcccccaaaaGCCGATAGGCGACCCCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGc                                                                                                                                                                              <  1:691610/232‑1 (MQ=255)                                             cccccATCCCTTACCCCAAAAGCCGATAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGc                                                                                                                                                                              <  1:326713/248‑1 (MQ=255)                                               gCCATCACTTACTCCACAAGCCGACAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGCTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTacgt                                                                                                                                                                                                                      >  1:785492/1‑206 (MQ=255)                                                     cttccccccccaacccGATGGGCGACACCGTGGCGTCCACTGAAATCCGGCACCTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcactac                                                                                                                                                                      <  1:388168/237‑4 (MQ=255)                                                      cTTACTCCACAAGCCGATAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcacta                                                                                                                                                                       >  1:719373/1‑247 (MQ=255)                                                       ttcccccaaaaGCCGATAGGCGACCCCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcactactt                                                                                                                                                                    <  1:528128/242‑6 (MQ=255)                                                                  gCCGATAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGtt                                                                                                                                                         >  1:734160/1‑251 (MQ=255)                                                                   ccGATAGGCGACCCCGTGGCGTCCACTGGAATCCGGCAACTCTTGACCCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcac                                                                                                                                                                         <  1:296831/234‑1 (MQ=255)                                                                      aTAGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGt                                                                                                                                                     >  1:535475/1‑251 (MQ=255)                                                                        aGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATGccaagt                                                                                                                                                                                                                                  >  1:266142/1‑169 (MQ=255)                                                                        aGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATg                                                                                                                                                                                                       >  1:761762/1‑199 (MQ=255)                                                                        aGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGAt                                                                                                                                                                                   <  1:819817/219‑1 (MQ=255)                                                                        aGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGttt                                                                                                                                                   >  1:708082/1‑251 (MQ=255)                                                                        aGGCGACACCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGttt                                                                                                                                                   >  1:475453/1‑251 (MQ=255)                                                                             acacCGTGGCGTCCACTGGAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGAt                                                                                                                                              >  1:60546/1‑251 (MQ=255)                                                                                       gtccactggAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAg                                                                                                                                     >  1:96831/9‑250 (MQ=255)                                                                                         ccactggAATCCGGCAACTCTTGAACCCGACAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGca                                                                                                                                   >  1:642226/7‑250 (MQ=255)                                                                                          cactggAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcactacttgtccc                                                                                                                                                               >  1:514740/6‑211 (MQ=255)                                                                                          cactggAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGcaca                                                                                                                                 >  1:30108/6‑251 (MQ=255)                                                                                            ctggAATCCGGCAACTCTTGACCCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcactacttgtcc                                                                                                                                                                <  1:207562/215‑10 (MQ=255)                                                                                            ctggAATCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCCCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcactac                                                                                                                                                                      >  1:728011/4‑209 (MQ=255)                                                                                                 aTCCGGCAACTCTTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCgg                                                                                                                          >  1:223497/1‑251 (MQ=255)                                                                                                          ctcttgaccccGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGc                                                                                                                                                                              <  1:142639/182‑1 (MQ=255)                                                                                                          ctctTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGAt                                                                                                                                                                                   >  1:779684/1‑185 (MQ=255)                                                                                                          ctctTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGc                                                                                                                  >  1:21997/1‑250 (MQ=255)                                                                                                           tctTGAACCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGccc                                                                                                                <  1:468642/251‑1 (MQ=255)                                                                                                            cttgaaCCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGccct                                                                                                               <  1:150248/247‑2 (MQ=255)                                                                                                               gaaCCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcactacttgtc                                                                                                                                                                 >  1:800375/2‑190 (MQ=255)                                                                                                                aaCCCGCCAATCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACag                                                                                                           <  1:58847/251‑1 (MQ=255)                                                                                                                        aaTCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGt                                                                                                     >  1:202034/1‑249 (MQ=255)                                                                                                                         aTCCAAATCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACag                                                                                                           <  1:477967/242‑1 (MQ=255)                                                                                                                             aaaTCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACAt                                                                                              <  1:417969/251‑1 (MQ=255)                                                                                                                             aaaTCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACAt                                                                                              <  1:401937/251‑1 (MQ=255)                                                                                                                             aaaTCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACAt                                                                                              <  1:654953/251‑1 (MQ=255)                                                                                                                                tCGTTGTGATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGcgtacgta                                                                                                                                                                                                                     <  1:821997/129‑1 (MQ=255)                                                                                                                                     gtgATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCgg                                                                                      >  1:559469/1‑251 (MQ=255)                                                                                                                                      tgATAACCGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGc                                                                                                        <  1:480465/232‑1 (MQ=255)                                                                                                                                             cGCTCGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTAttggtg                                                                              <  1:403740/251‑5 (MQ=255)                                                                                                                                                 cGCCGGGATAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGa                                                                          >  1:228883/1‑251 (MQ=255)                                                                                                                                                         tAATTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACag                                                                                                           >  1:457316/1‑210 (MQ=255)                                                                                                                                                          aaTTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcac                                                                                                                                                                         >  1:244312/1‑147 (MQ=255)                                                                                                                                                          aaTTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCa                                                                 >  1:716131/1‑251 (MQ=255)                                                                                                                                                           aTTAACCATTTTTCACCATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGAt                                                                                                                                                                                   >  1:613611/1‑136 (MQ=255)                                                                                                                                                                     tttcaccATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTg                                                      <  1:76693/245‑1 (MQ=255)                                                                                                                                                                          ccATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAg                                                                                                                                                            >  1:721173/2‑144 (MQ=255)                                                                                                                                                                          ccATTAATGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGtt                                                                                                                                                    >  1:95549/2‑152 (MQ=255)                                                                                                                                                                                aTGGTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGtattgtcgg                                            >  1:310669/1‑245 (MQ=255)                                                                                                                                                                                  ggTTAAGATTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATcactac                                                                                                                                                                      >  1:282663/1‑123 (MQ=255)                                                                                                                                                                                        gactgtgCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGtattgtcggctcggttgg                                   >  1:85493/4‑237 (MQ=255)                                                                                                                                                                                         aTTGTGCGCGGGATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGc                                                                                                        >  1:521410/1‑181 (MQ=255)                                                                                                                                                                                                   ggATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGc                                                                                                        <  1:542566/171‑1 (MQ=255)                                                                                                                                                                                                   ggATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACAt                                                                                              <  1:175057/181‑1 (MQ=255)                                                                                                                                                                                                   ggATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCaaaaa                        >  1:652987/1‑251 (MQ=255)                                                                                                                                                                                                   ggATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCaaaaa                        >  1:7784/1‑251 (MQ=255)                                                                                                                                                                                                   ggATGATGCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCaaaaa                        >  1:719808/1‑251 (MQ=255)                                                                                                                                                                                                        atgCCATACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCa                   >  1:212184/1‑251 (MQ=255)                                                                                                                                                                                                          gccatACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACAt                                                                                              >  1:813933/5‑174 (MQ=255)                                                                                                                                                                                                          gccatACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCatg                 >  1:379888/5‑249 (MQ=255)                                                                                                                                                                                                           ccatACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAGCATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATAc                     >  1:430033/4‑246 (MQ=255)                                                                                                                                                                                                           ccatACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCatgg                >  1:550197/4‑248 (MQ=255)                                                                                                                                                                                                             atACATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCATGGGc              <  1:662056/250‑1 (MQ=255)                                                                                                                                                                                                                cATGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATAc                     >  1:640282/1‑241 (MQ=255)                                                                                                                                                                                                                  tGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGt                                                                                                                      >  1:397797/1‑142 (MQ=255)                                                                                                                                                                                                                  tGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGccctac                                                                                                             >  1:133447/1‑148 (MQ=255)                                                                                                                                                                                                                  tGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCATGGGCTGCgg         >  1:228929/1‑251 (MQ=255)                                                                                                                                                                                                                  tGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCATGGGCTGCgg         >  1:240786/1‑251 (MQ=255)                                                                                                                                                                                                                  tGTTTACATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCATGGGCTGCgg         >  1:526385/1‑251 (MQ=255)                                                                                                                                                                                                                        cATGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCAc                                >  1:301677/1‑222 (MQ=255)                                                                                                                                                                                                                          tGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGccctac                                                                                                             >  1:138340/1‑140 (MQ=255)                                                                                                                                                                                                                          tGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCATGGGCTGCGGCTTTCGc  >  1:190278/1‑250 (MQ=255)                                                                                                                                                                                                                          tGATAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGTATTGTCGGCTCGGTTGGCACCGCAAAAATACCATGGGCTGCGGCTTTCGc  >  1:479441/1‑250 (MQ=255)                                                                                                                                                                                                                            aTAACGGTCCGGCACAATG‑CCAAGTCGGGCGTACGTATCATTGCGTAAATGTGCGGTAATCACGTTAGGATTAGGCATCACTACTTGTCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCTACAGAGCGGTCTAACATTGCACCGGTATTGGTGCCGAAAAAATGCACGCCGGCAGTGGtattg                                                <  1:166761/202‑2 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             TGCCGTACCGGCGTTAAGCTTAGTTAAAAAATAACCCTGATGCGCTGCCATCACTTACTCCACAAGCCGATAGGCGACACCATGGCGTCCGCTCGAATCCGGCAACTCTTTAACCCGCCAATCCAAATCGTTGTGATAGCCGCTCGCCGGGATAATTAACCATTTTTGAGCATTAATGGTTAAGATTGTGCGCGGGATGATGCCGTACATGTTTACATGATAACGGTCGGGTACAATGCCCAAG‑CGGGCGTAAGTATCATTGCGCAAATGCGCGGTGATGACGTTAGGATTGGGCATCACCACCTGCCCCAAGGTTGAGTTTGAGATAGCGGTTAGCACATCATCGGCAGTATGCCCGACAGAGCGGTCTAACATTGCCCCGGTATTAGCGCCGAAAAAATGCACTCCGGCAGTGGTATTATCAGATCTGTTGGCACCGCAAAAATACCACGGGCTGCGGCTTTCGC  >  1/93821‑94287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑