Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,528,208	A→C	F24L (TTT→TTG)	unknown ←	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,528,208	0	A	C	100.0%	218.5 / NA	76	F24L (TTT→TTG)	unknown	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/49);  total (27/49)

GCATAAAGTAATAAGAAAAGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAAAAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCG‑CATGCATGACCAATACTCTATCGGACATATGTTCAATTTCTTCTAAATCGGAAGAAATAAAC  >  1/1527977‑1528435                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     gCATAAAGTAATAAGAAAAGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGcc                                                                                                                                                                                                                                 <  1:18066/237‑1 (MQ=255)  cATAAAGTAATAAGAAAAGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGaa                                                                                                                                                                                                                   <  1:778986/250‑1 (MQ=255)  cATAAAGTAATAAGAAAAGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGaa                                                                                                                                                                                                                   <  1:75805/250‑1 (MQ=255)      aaGTAATAAGAAAAGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGt                                                                                                                                                                                                               >  1:284861/1‑250 (MQ=255)                aaaaGCGGAGACTAAACCATAACCGGCGTTTAGCAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAATCCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    <  1:619307/251‑1 (MQ=255)                aaaaGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    <  1:741353/251‑1 (MQ=255)                aaaaGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    <  1:513198/251‑1 (MQ=255)                aaaaGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    <  1:280088/251‑1 (MQ=255)                    gCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTAt                                                                                                                                                                                                 >  1:708486/1‑250 (MQ=255)                         gaCTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    <  1:552520/242‑1 (MQ=255)                          aCTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAAt                                                                                                                                                                                          <  1:864072/251‑1 (MQ=255)                               aCCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGtt                                                                                                                                                                                     <  1:547512/251‑1 (MQ=255)                               aCCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGAACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGtt                                                                                                                                                                                     <  1:519400/251‑1 (MQ=255)                                ccATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGaa                                                                                                                                                                                                                   <  1:559832/220‑1 (MQ=255)                                                 cAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCca                                                                                                                                                                   <  1:288753/251‑1 (MQ=255)                                                     cTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    <  1:780854/214‑1 (MQ=255)                                                       gCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    >  1:389606/1‑212 (MQ=255)                                                       gCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCg                                                                                                                                                             >  1:96345/1‑251 (MQ=255)                                                                   cGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 <  1:205350/251‑1 (MQ=255)                                                                   cGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 <  1:449983/251‑1 (MQ=255)                                                                   cGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 <  1:431783/251‑1 (MQ=255)                                                                     cacaAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCg                                                                                                                                               <  1:551929/251‑1 (MQ=255)                                                                           gCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTccc                                                                                                                                                                    <  1:580287/224‑1 (MQ=255)                                                                            ccccATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGc                                                                                                                                                                                <  1:611734/211‑1 (MQ=255)                                                                              ccATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTa                                                                                                                                         >  1:198088/1‑248 (MQ=255)                                                                                aTGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGcc                                                                                                                                    <  1:262270/251‑1 (MQ=255)                                                                                 tGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGaa                                                                                                                                                                                                                   <  1:226934/171‑1 (MQ=255)                                                                                 tGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 >  1:21882/1‑237 (MQ=255)                                                                                 tGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCg                                                                                                                                   >  1:606765/1‑251 (MQ=255)                                                                                 tGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCg                                                                                                                                   >  1:825357/1‑251 (MQ=255)                                                                                    tAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAAc                                                                                                                                <  1:736447/251‑1 (MQ=255)                                                                                       aaCCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGc                                                                                                                              <  1:676146/250‑1 (MQ=255)                                                                                            aCGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 >  1:465317/1‑226 (MQ=255)                                                                                                     tCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAtt                                                                                                               >  1:617907/1‑251 (MQ=255)                                                                                                     tCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAtt                                                                                                               >  1:278298/1‑251 (MQ=255)                                                                                                        aTGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTccc                                                                                                                                                                    <  1:593494/195‑1 (MQ=255)                                                                                                         tGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAttgttg                                                                                                           <  1:796283/251‑1 (MQ=255)                                                                                                         tGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAttgttg                                                                                                           <  1:12509/251‑1 (MQ=255)                                                                                                         tGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAttgttg                                                                                                           <  1:7855/251‑1 (MQ=255)                                                                                                         tGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAttgttg                                                                                                           <  1:185919/251‑1 (MQ=255)                                                                                                         tGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAttgttg                                                                                                           <  1:645107/251‑1 (MQ=255)                                                                                                           ttGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTgg                                                                                                          <  1:663164/250‑1 (MQ=255)                                                                                                           ttGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGt                                                                                                         <  1:504927/251‑1 (MQ=255)                                                                                                                ggTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTcc                                                                                                                                                      >  1:560376/1‑201 (MQ=255)                                                                                                                ggTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTaa                                                                                                    >  1:583079/1‑251 (MQ=255)                                                                                                                  ttAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATc                                                                                                  <  1:262253/251‑1 (MQ=255)                                                                                                                         gACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCa                                                                                            >  1:770653/1‑250 (MQ=255)                                                                                                                             gaaGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGc                                                                                                                                                                                                                           <  1:620792/118‑1 (MQ=255)                                                                                                                              aaGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 <  1:330170/192‑1 (MQ=255)                                                                                                                                                gCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTg                                                                     >  1:603799/1‑250 (MQ=255)                                                                                                                                                 cAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGaa                                                                                <  1:305586/238‑1 (MQ=255)                                                                                                                                                 cAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAAttt                                                                      <  1:703270/248‑1 (MQ=255)                                                                                                                                                                cgcgGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGAcc                                                     <  1:716929/250‑1 (MQ=255)                                                                                                                                                                    gAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 >  1:482927/1‑154 (MQ=255)                                                                                                                                                                    gAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTAc                                                                                                                                        >  1:799723/1‑163 (MQ=255)                                                                                                                                                                    gAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTAc                                                                                                                                        >  1:692976/1‑163 (MQ=255)                                                                                                                                                                      aTTAAACACCATGCTTAACGTTTGCAGATGAAAGCCGGTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGa                                                                                 <  1:569943/217‑1 (MQ=255)                                                                                                                                                                      aTTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATcc                                                                                                                                                                                               >  1:762657/1‑106 (MQ=255)                                                                                                                                                                      aTTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 <  1:427903/152‑1 (MQ=255)                                                                                                                                                                      aTTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 <  1:413638/152‑1 (MQ=255)                                                                                                                                                                      aTTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGt                                                                                                                                          >  1:349170/1‑159 (MQ=255)                                                                                                                                                                      aTTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATActc                                              <  1:400218/251‑1 (MQ=255)                                                                                                                                                                      aTTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATAct                                               >  1:652687/1‑250 (MQ=255)                                                                                                                                                                         aaaCACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTAt                                           <  1:421942/251‑1 (MQ=255)                                                                                                                                                                                 tGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTAcc                                                                                                                                                 <  1:438012/141‑1 (MQ=255)                                                                                                                                                                                 tGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATAttgttg                                                                                                           <  1:542639/179‑1 (MQ=255)                                                                                                                                                                                 tGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAAtt                                                                       >  1:224279/1‑215 (MQ=255)                                                                                                                                                                                 tGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTATCGGACata                                   >  1:350016/1‑251 (MQ=255)                                                                                                                                                                                 tGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTATCGGACata                                   >  1:47379/1‑251 (MQ=255)                                                                                                                                                                                 tGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTATCGGACata                                   >  1:260073/1‑251 (MQ=255)                                                                                                                                                                                           ttGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTATCGGACATATGTTCAAttt                         <  1:491817/251‑1 (MQ=255)                                                                                                                                                                                                gATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTAt                                           <  1:709358/228‑1 (MQ=255)                                                                                                                                                                                                      aGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTAt                                           >  1:785532/1‑222 (MQ=255)                                                                                                                                                                                                           gTTTCCGACAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTATCGGACATATGTTc                              <  1:780745/231‑1 (MQ=255)                                                                                                                                                                                                                   cAAAACCTAACAGTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCGTCATGCATGACCAATACTCTATCGGACATATGTTCAATTTCTTCTAAATCGGAAGAAATAAAc  <  1:842841/251‑1 (MQ=255)                                                                                                                                                                                                                               gTAAAAATAACAAGCCAATGGCAATAAGAATGGTCAGTTCACGAt                                                                                                                                                                                                    >  1:286142/1‑45 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     GCATAAAGTAATAAGAAAAGCGGAGACTAAACCATAACCGGCGTTTAACAAGCTGCCGACAATGACCGCACAAAGCCCCATGGTAGAACCGACGGACACGTCGATGTTGCGGGTTAAAATGACGAAAGTGGCGCCAATCGCCAGCAAAATCACGATTTGCGCGGAATTAAACACCATGCTTAACGTTTGCAGATGAAAGCC‑GTTTCCGACAAAACCTAACAGTAAAAATAAAAAGCCAATGGCAATAAGAATGGTCAGTTCACGATTATCCTGAATCAGTTTCAGCATCAATTTTCCCATCATCGTCTCTCCTTACCCGTGCGTACTGCCGAACGCCATTTGCATGATATTGTTGGTATTAATCGCCTCACCGACCAAAGAATGGCTAATTTGCCCG‑CATGCATGACCAATACTCTATCGGACATATGTTCAATTTCTTCTAAATCGGAAGAAATAAAC  >  1/1527977‑1528435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑