Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,480,321 G→A R150H (CGC→CAC)  unknown → Inner membrane transport permease YhhJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,480,3210GA98.6% 192.0 / NA 69R150H (CGC→CAC) unknownInner membrane transport permease YhhJ
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (30/38);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCGCCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCCACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCGCCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACGGGAGCGCGGCACGCTGGAACATATTTTGGTGATGCCCGTACGCTCCAGCGAAATTGCTATGGCGAAAATCGTGGCGAACGAACTGGTGTT  >  1/1480081‑1480567
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      
gCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                                                                                                                                                                               >  1:673450/1‑250 (MQ=255)
 ccTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGc                                                                                                                                                                                                                                              <  1:168582/250‑1 (MQ=255)
      ttCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAAc                                                                                                                                                                                                                                        <  1:131833/251‑1 (MQ=255)
              aaCTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACt                                                                                                                                                                                                                                <  1:844904/251‑1 (MQ=255)
                  tCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                                                                                                                                                                               <  1:771660/232‑1 (MQ=255)
                  tCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCa                                                                                                                                                                                                                                    >  1:239688/1‑243 (MQ=255)
                       aaaGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                                                                                                                                                                       <  1:317951/251‑1 (MQ=255)
                         aGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACgg                                                                                                                                                                                                                                      <  1:811215/234‑1 (MQ=255)
                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTc                                                                                                                                                                                                                           <  1:687576/244‑1 (MQ=255)
                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAc                                                                                                                                                                                                                        >  1:456730/1‑247 (MQ=255)
                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACcca                                                                                                                                                                                                                     >  1:7486/1‑250 (MQ=255)
                          gTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACcca                                                                                                                                                                                                                     >  1:38761/1‑250 (MQ=255)
                                aCAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCGCCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTaa                                                                                                                                                                                                              <  1:157977/251‑1 (MQ=255)
                                 cAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTaa                                                                                                                                                                                                              <  1:737075/250‑1 (MQ=255)
                                  aaTTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCGATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAAtg                                                                                                                                                                                                            <  1:145630/251‑1 (MQ=255)
                                    ttCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAc                                                                                                                                                                                                                        <  1:133334/237‑1 (MQ=255)
                                     tCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGc                                                                                                                                                                                                         <  1:84154/251‑1 (MQ=255)
                                       aTGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                                                                                                                                                                       >  1:150973/1‑235 (MQ=255)
                                               caGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAAcc                                                                                                                                                                                                >  1:808992/1‑250 (MQ=255)
                                                          ccTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                                                                                                                                                                       >  1:379171/1‑216 (MQ=255)
                                                             gATGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTa                                                                                                                                                                                 >  1:819625/1‑251 (MQ=255)
                                                               tGGACACAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAAc                                                                                                                                                                               <  1:104594/251‑1 (MQ=255)
                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTc                                                                                                                                                                                                                               <  1:326392/200‑1 (MQ=255)
                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTgg                                                                                                                                                                            >  1:786421/1‑251 (MQ=255)
                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTgg                                                                                                                                                                            >  1:41641/1‑251 (MQ=255)
                                                                  acacAGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTgg                                                                                                                                                                            >  1:591876/1‑251 (MQ=255)
                                                                     caGGAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGttt                                                                                                                                                                         >  1:726217/1‑251 (MQ=255)
                                                                       ggAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAcc                                                                                                                                                                                                                       <  1:585140/203‑1 (MQ=255)
                                                                       ggAGAATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTAt                                                                                                                                                                       >  1:32574/1‑251 (MQ=255)
                                                                          gaATATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATggg                                                                                                                                                                    >  1:650771/1‑251 (MQ=255)
                                                                           aaTATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                                                                                                                                                                               >  1:525723/1‑175 (MQ=255)
                                                                           aaTATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATggg                                                                                                                                                                    <  1:315666/250‑1 (MQ=255)
                                                                           aaTATACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATggg                                                                                                                                                                    <  1:498288/250‑1 (MQ=255)
                                                                            atatACGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTtat                                                                                                                                                                                                    <  1:666591/217‑1 (MQ=255)
                                                                              ataCGTTCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTc                                                                                                                                                                                      >  1:770648/1‑229 (MQ=255)
                                                                                    tCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAAc                                                                                                                                                          <  1:187706/251‑1 (MQ=255)
                                                                                    tCATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAAc                                                                                                                                                          <  1:629203/251‑1 (MQ=255)
                                                                                     cATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAg                                                                                                                                                                                                                                               >  1:565764/1‑165 (MQ=255)
                                                                                     cATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACa                                                                                                                                                         <  1:800254/251‑1 (MQ=255)
                                                                                     cATTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACa                                                                                                                                                         <  1:112702/251‑1 (MQ=255)
                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAccc                                                                                                                                                                                                                      >  1:289409/1‑189 (MQ=255)
                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAt                                                                                                                                                        <  1:764495/251‑1 (MQ=255)
                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAt                                                                                                                                                        >  1:268483/1‑251 (MQ=255)
                                                                                      aTTTTAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACAt                                                                                                                                                        >  1:472676/1‑251 (MQ=255)
                                                                                       ttttAGATATTCCACCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACgg                                                                                                                                                                                                                                      <  1:374735/172‑1 (MQ=255)
                                                                                                          ttATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACa                                                                                                                                                         >  1:129695/1‑230 (MQ=255)
                                                                                                          ttATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATttat                                                                                                                                    >  1:560/1‑251 (MQ=255)
                                                                                                               aaCGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAAcc                                                                                                                               <  1:837937/251‑1 (MQ=255)
                                                                                                               aaCGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAAcc                                                                                                                               <  1:468899/251‑1 (MQ=255)
                                                                                                               aaCGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAAc                                                                                                                                <  1:280815/250‑1 (MQ=255)
                                                                                                               aaCGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAAc                                                                                                                                <  1:855194/250‑1 (MQ=255)
                                                                                                                 cGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTgg                                                                                                                                                   >  1:383334/1‑229 (MQ=255)
                                                                                                                 cGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTg                                                                                                                                           <  1:651870/237‑1 (MQ=255)
                                                                                                                  gTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATAc                                                                                                                                                                                                                                 <  1:462951/150‑1 (MQ=255)
                                                                                                                      tGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGc                                                                                                                        <  1:128838/251‑1 (MQ=255)
                                                                                                                         aCTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCtgg                                                                                                                     <  1:604460/251‑1 (MQ=255)
                                                                                                                         aCTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCtgg                                                                                                                     <  1:760814/251‑1 (MQ=255)
                                                                                                                                  gCGTAACCCGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATggg                                                                                                                                                                    <  1:792106/195‑1 (MQ=255)
                                                                                                                                          cGCTGATTCAGTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATATCCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCAtt                                                                                                    <  1:688652/251‑1 (MQ=255)
                                                                                                                                                    gTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATAc                                                                                                                                                                                                                                 >  1:760517/1‑116 (MQ=255)
                                                                                                                                                    gTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACggg                                                                                          >  1:891899/1‑251 (MQ=255)
                                                                                                                                                    gTTATTATCCGACGCGACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACggg                                                                                          >  1:412280/1‑251 (MQ=255)
                                                                                                                                                                        cAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACGGGAGCGCGGCACGCTGGAACat                                                                      <  1:368723/251‑1 (MQ=255)
                                                                                                                                                                                              ttCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACGGGAGCGCGGCACGCTGGAACATATTTTGGTGATGCCCGTACGc                                                 <  1:686870/250‑1 (MQ=255)
                                                                                                                                                                                                ccGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACGGGAGCGCGGCACGCTGGAACATATTTTGGTGATGCCCGTACGCTcc                                              <  1:866219/251‑1 (MQ=255)
                                                                                                                                                                                                 cGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACGGGAGCGCGGCACGCTGGAACATATTTTGGTGATGCCCGTACGCTCCa                                             >  1:496330/1‑251 (MQ=255)
                                                                                                                                                                                                  gCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAAt                                                                                                                                        >  1:780674/1‑159 (MQ=255)
                                                                                                                                                                                                                                aTTAACGATTTTCTGCACCTTCAAAGCAATAACGGCATACTCATTCAAc                                                                                                                                                                                                                        <  1:564400/49‑1 (MQ=255)
                                                                                                                                                                                                                                            cTGCACCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACGGGAGCGCGGCACGCTGGAACATATTTTGGTGATGCCCGTACGCTCCAGCGAAATTGCTATGGCGAAAATCGTGGCGAACGAACTGGTGtt  >  1:582984/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      
GCCTGATTCCGCCGAACTTCCGTAAAGTGCATACAATTCATGCACAACAGGCGGATCGCCTGATGGACACAGGAGAATATACGTTCATTTTAGATATTCCGCCGAATTATGAACGTGATGTACTTGCGGGGCGTAACCCGCTGATTCAGTTATTATCCGACGCCACGGCAATGACGCAAGCCGCCATCGGTTCCGCTTATATTTCACAAATTTTTCACGGTGAAATTAACGATTTTCTGCGCCTTCAAAGCAATAACGGCATACTCATTCAACCCACCATTAATGTGCTTTATAACCCGAATTTTTCCGGTAACTGGTTTATGGGCGGCATGAACATTGTGGGCTATTTGAATTTATTAACCATGTTGCTGGTGGGCGCCGCTGTCATTCGGGAACGGGAGCGCGGCACGCTGGAACATATTTTGGTGATGCCCGTACGCTCCAGCGAAATTGCTATGGCGAAAATCGTGGCGAACGAACTGGTGTT  >  1/1480081‑1480567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑