Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,316	G→A	G58E (GGA→GAA)	unknown →	HlyD‑like membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,316	0	G	A	100.0%	190.9 / NA	68	G58E (GGA→GAA)	unknown	HlyD‑like membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/33);  total (35/33)

GTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGCATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGGAATTTTGGTGCAGGAAGGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACAC  >  1/1476086‑1476560                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTa                                                                                                                                                                                                                                  >  1:47124/1‑251 (MQ=255)   ttttACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAAt                                                                                                                                                                                                                                <  1:302839/251‑1 (MQ=255)     ttACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGa                                                                                                                                                                                                                              >  1:7945/1‑251 (MQ=255)       aCCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAg                                                                                                                                                                                                                            <  1:146489/251‑1 (MQ=255)       aCCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAg                                                                                                                                                                                                                            <  1:226584/251‑1 (MQ=255)       aCCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAg                                                                                                                                                                                                                            <  1:774035/251‑1 (MQ=255)           gATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTaaa                                                                                                                                                                                                                        >  1:75865/1‑251 (MQ=255)              ctctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACg                                                                                                                                                                                                                     >  1:103309/1‑251 (MQ=255)              ctctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACg                                                                                                                                                                                                                     >  1:559666/1‑251 (MQ=255)                ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGaa                                                                                                                                                                                                                                      >  1:311353/1‑232 (MQ=255)                ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGc                                                                                                                                                                                                                    >  1:159844/1‑250 (MQ=255)                ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCa                                                                                                                                                                                                                   >  1:729389/1‑251 (MQ=255)                ctATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCa                                                                                                                                                                                                                   >  1:175796/1‑251 (MQ=255)                       cATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGCATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCAACCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGCAAAACGCAATCAAc                                                                                                                                                                                                             >  1:669512/1‑250 (MQ=255)                           cGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTaa                                                                                                                                                                                                                                 <  1:763034/226‑1 (MQ=255)                           cGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCa                                                                                                                                                                                                           >  1:256207/1‑248 (MQ=255)                                     gcgTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGGAATGAAGTAAAACGCAATCAACCACTGGCACGGCttt                                                                                                                                                                                              >  1:564856/1‑251 (MQ=255)                                              tAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGc                                                                                                                                                                                                      <  1:3087/234‑1 (MQ=255)                                                          gaTGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTaa                                                                                                                                                                                                                                 >  1:90373/1‑195 (MQ=255)                                                               aaGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGtt                                                                                                                                                                    <  1:814626/251‑1 (MQ=255)                                                                        atTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGca                                                                                                                                                           >  1:646707/1‑251 (MQ=255)                                                                         tttCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGcac                                                                                                                                                          <  1:82845/251‑1 (MQ=255)                                                                                    gTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAg                                                                                                                                               >  1:22785/1‑251 (MQ=255)                                                                                     tttCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAg                                                                                                                                               <  1:43110/250‑1 (MQ=255)                                                                                       tCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCGCAGGCACAAAAGaaa                                                                                                                                            <  1:32316/251‑1 (MQ=255)                                                                                       tCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGaaa                                                                                                                                            <  1:237017/251‑1 (MQ=255)                                                                                          ttattaTCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAgg                                                                                                                                                       >  1:365812/1‑237 (MQ=255)                                                                                          ttattaTCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACgg                                                                                                                                         <  1:620126/251‑1 (MQ=255)                                                                                          ttattaTCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACgg                                                                                                                                         >  1:610702/1‑251 (MQ=255)                                                                                              tatCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAAcc                                                                                                                                                                                                            <  1:139499/180‑1 (MQ=255)                                                                                                           ggTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTcc                                                                                                                                                                                         >  1:744872/1‑186 (MQ=255)                                                                                                            gTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTccgc                                                                                                                                                               >  1:612061/1‑211 (MQ=255)                                                                                                                  ccATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGcc                                                                                                                 <  1:573440/251‑1 (MQ=255)                                                                                                                                           ggCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCcaa                                                                                         >  1:365610/1‑250 (MQ=255)                                                                                                                                           ggCCAGTGAACTGAACGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGAGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTttcttc                                                                                                                                                                                          >  1:797683/1‑153 (MQ=255)                                                                                                                                                 tgaactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCcaacaac                                                                                     <  1:567793/248‑1 (MQ=255)                                                                                                                                                  gaactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGt                                                                                 >  1:560991/1‑251 (MQ=255)                                                                                                                                                   aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTATAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc                                                                                <  1:652558/251‑1 (MQ=255)                                                                                                                                                   aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc                                                                                <  1:661309/251‑1 (MQ=255)                                                                                                                                                   aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc                                                                                <  1:40427/251‑1 (MQ=255)                                                                                                                                                   aactgaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTACTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTc                                                                                <  1:112555/251‑1 (MQ=255)                                                                                                                                                       gaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTccgc                                                                                                                                                               >  1:339351/1‑168 (MQ=255)                                                                                                                                                       gaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGcac                                                                                                                                                    >  1:767207/1‑179 (MQ=255)                                                                                                                                                       gaacGGTATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACg                                                                            >  1:463910/1‑251 (MQ=255)                                                                                                                                                            gtaTTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTccgc                                                                                                                                                               <  1:68985/161‑1 (MQ=255)                                                                                                                                                            gtaTTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACg                                                                       >  1:171996/3‑251 (MQ=255)                                                                                                                                                            gtaTTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACg                                                                       >  1:580098/3‑251 (MQ=255)                                                                                                                                                            gtaTTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACg                                                                       >  1:80052/3‑251 (MQ=255)                                                                                                                                                            gtaTTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAAGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAg                                                                                  >  1:436390/3‑240 (MQ=255)                                                                                                                                                                           aCGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAAt                                                        <  1:799093/251‑1 (MQ=255)                                                                                                                                                                           aCGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAAt                                                        <  1:483530/251‑1 (MQ=255)                                                                                                                                                                                      gAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCAttt                                                                                                                                                                                  >  1:525401/1‑118 (MQ=255)                                                                                                                                                                                               cGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAGGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTgcgc                                      >  1:508832/1‑249 (MQ=255)                                                                                                                                                                                                         ttGCCACCCTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAAt                             >  1:334544/1‑248 (MQ=255)                                                                                                                                                                                                                ccTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAAt                                                             <  1:204511/209‑1 (MQ=255)                                                                                                                                                                                                                ccTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCaa                   >  1:610154/1‑251 (MQ=255)                                                                                                                                                                                                                 cTTTATCCCGGCCGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGCTGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAAt                                                        >  1:424099/1‑213 (MQ=255)                                                                                                                                                                                                                            ccGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAg       <  1:556335/251‑1 (MQ=255)                                                                                                                                                                                                                             cGTGTAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGa      <  1:487025/251‑1 (MQ=255)                                                                                                                                                                                                                              gtgtAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAg                                                                                                                                               <  1:772595/113‑1 (MQ=255)                                                                                                                                                                                                                              gtgtAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTg                                         <  1:353790/215‑1 (MQ=255)                                                                                                                                                                                                                              gtgtAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTg                                         <  1:685262/215‑1 (MQ=255)                                                                                                                                                                                                                              gtgtAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGaa     >  1:601843/1‑251 (MQ=255)                                                                                                                                                                                                                               tgtAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAac    <  1:746004/251‑1 (MQ=255)                                                                                                                                                                                                                               tgtAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAac    <  1:616452/251‑1 (MQ=255)                                                                                                                                                                                                                                gtAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAac    <  1:619325/250‑1 (MQ=255)                                                                                                                                                                                                                                 tAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGTGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAacac  <  1:427609/251‑1 (MQ=255)                                                                                                                                                                                                                                 tAGAAGAAATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAacac  <  1:415632/251‑1 (MQ=255)                                                                                                                                                                                                                                   gaagaaATTTTGGTGCAGGAAAGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGACAATCTGATTTCAAGCAGCGAATTAGAacac  <  1:281967/249‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GTTTTTACCCGATCTCTATAATCATTCGCATAAACCGCGTTAAAGTAAGGATACAGAGATGAAAGCAAAATATTTCGTGTTCGGTTTCATTATTATCGGTGCCGTCGGTTTCGCCATTTGGAAAAACAGGCAATTACAGGCCAGTGAACTGAACGGCATTGCGGCAGTTAACGGACGTTTGGAATTAAAACGGCTGGACATTGCCACCCTTTATCCCGGCCGTGTAGAAGGAATTTTGGTGCAGGAAGGTAATGAAGTAAAACGCAATCAACCACTGGCACGGCTTTCTTCCAGCATTTCGCAAGCGCAGGTTTCCGCCGCACAGGCACAAAAGAAACGGGCGGAAGAATCCGTTGCCCGCGCCCTATCAGAAATTGATGCCCGCCAACAACAAGTCAACGTAACGAAATTGGAATTGAATAACGCACAAAAATTGCGCCTCGATAATCTGATTTCAAGCAGCGAATTAGAACAC  >  1/1476086‑1476560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑