Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,466,907	A→G	L692P (CTG→CCG)	unknown ←	DNA topoisomerase I (EC 5.99.1.2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,466,907	0	A	G	100.0%	143.9 / NA	51	L692P (CTG→CCG)	unknown	DNA topoisomerase I (EC 5.99.1.2)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/19);  total (32/19)

AATCGGTTCTTCTTTCGGTGGCGCCACTTCGCCGTTTTTCAGAATTTTGCGCGTATTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCAGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACCAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGAATACGCCTGTACTGGCGGTTCTGATCGCCATATTG  >  1/1466705‑1467152                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      attCGGTTCTTCTTTCGGTGGCGCCACTTCGCCGTTTTTCAGAATTTTGCGCGTATTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAAc                                                                                                                                                                                                       <  1:702217/249‑1 (MQ=255) aaTCGGTTCTTCTTTCGGTGGCGCCATTTCGCCGTTTTTCAGAATTTTGCGCGTACTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAg                                                                                                                                                                                                                                 <  1:423590/225‑1 (MQ=255) aaTCGGTTCTTCTTTCGGTGGCGCCACTTCGCCGTTTTTCAGAATTTTGCGCGTATTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCTCCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAAc                                                                                                                                                                                                       <  1:90683/251‑1 (MQ=255)   tCGGTTCTTCTTTCGTTGGCGCCTCTTCGCCGTTTTTCAGAATTTTGCGCGTATTATCGCATGGTGTACACGCAATAGACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCGCCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTg                                                                                                                                                                                                     <  1:215416/251‑1 (MQ=255)      tttcttcTTTCGGTGGCGCCACTTCGCCGTTTTTCAGAATTTTGCGCGTATTAGCGCATTGTATACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTg                                                                                                                                                                                                                                               <  1:548833/205‑1 (MQ=255)           tcttTCGGTGGCGCCACTTCGCCGTTTTTCAGAATTTTGCGCGTATTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCg                                                                                                                                                                                              >  1:275915/1‑250 (MQ=255)                            ttCGCCGTTTTTCAGAATTTTGCGCGTATTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGc                                                                                                                                                                            <  1:855069/251‑1 (MQ=255)                                                     gTATTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCttt                                                                                                                                                   >  1:464302/1‑251 (MQ=255)                                                        ttatCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTcc                                                                                                                                                                                                  <  1:798402/201‑1 (MQ=255)                                                          atCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTATCGCTTTGGttt                                                                                                                                              >  1:689455/1‑251 (MQ=255)                                                             gCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGt                                                                                                                                                                                                                                >  1:240085/1‑166 (MQ=255)                                                                  gtgtACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGt                                                                                                                                                                 >  1:530904/1‑224 (MQ=255)                                                                  gtgtACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGAcgcg                                                                                                                                      >  1:61011/1‑251 (MQ=255)                                                                  gtgtACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGAcgcg                                                                                                                                      >  1:325233/1‑251 (MQ=255)                                                                  gtgtACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGAcgcg                                                                                                                                      >  1:847108/1‑251 (MQ=255)                                                                         cGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTAc                                                                                                                                                                                        >  1:839483/1‑194 (MQ=255)                                                                         cGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCa                                                                                                                               >  1:307200/1‑251 (MQ=255)                                                                         cGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCa                                                                                                                               >  1:369401/1‑251 (MQ=255)                                                                         cGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCa                                                                                                                               >  1:409987/1‑251 (MQ=255)                                                                           ccATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCaa                                                                                                                              >  1:467496/1‑250 (MQ=255)                                                                               ataCTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGtt                                                                                                                                                                                                                                                   >  1:766665/1‑129 (MQ=255)                                                                               ataCTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAAc                                                                                                                                                                                                       >  1:336115/1‑173 (MQ=255)                                                                               ataCTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACAt                                                                                                                         >  1:69550/1‑251 (MQ=255)                                                                               ataCTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACAt                                                                                                                         >  1:632041/1‑251 (MQ=255)                                                                                                  aGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCAt                                                                                                                                                                                   >  1:511793/1‑174 (MQ=255)                                                                                                   gTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAATGATTGGGCCGTCATAACC‑TTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAAc                                                                                                                                                                                                       >  1:171458/1‑152 (MQ=255)                                                                                                          gTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGt                                                                                              >  1:686688/1‑251 (MQ=255)                                                                                                          gTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGt                                                                                              >  1:265876/1‑251 (MQ=255)                                                                                                             cATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTaa                                                                                           <  1:734547/251‑1 (MQ=255)                                                                                                               tatCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGataa                                                                                                                                                                                                             >  1:115081/1‑135 (MQ=255)                                                                                                                atCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAAtgg                                                                                        <  1:187144/251‑1 (MQ=255)                                                                                                                                                    gTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGat                                                                                                                                                                                                               <  1:201723/96‑1 (MQ=255)                                                                                                                                                    gTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAg                                                     >  1:500518/1‑250 (MQ=255)                                                                                                                                                     tCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGcc                                                   <  1:10527/251‑1 (MQ=255)                                                                                                                                                     tCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGc                                                    >  1:444506/1‑250 (MQ=255)                                                                                                                                                           ccTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTc                                                                                                               >  1:69253/1‑185 (MQ=255)                                                                                                                                                               tAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCt                                         <  1:817496/251‑1 (MQ=255)                                                                                                                                                                 aTCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGc                                                                               >  1:175306/1‑211 (MQ=255)                                                                                                                                                                 aTCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTaa                                       >  1:566075/1‑251 (MQ=255)                                                                                                                                                                 aTCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTaa                                       >  1:156929/1‑251 (MQ=255)                                                                                                                                                                    ttAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGaa                                    <  1:525820/251‑1 (MQ=255)                                                                                                                                                                    ttAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGaa                                    <  1:687561/251‑1 (MQ=255)                                                                                                                                                                    ttAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGaa                                    <  1:443817/251‑1 (MQ=255)                                                                                                                                                                    ttAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGaa                                    <  1:898894/251‑1 (MQ=255)                                                                                                                                                                     tAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGAAt                                   <  1:40468/251‑1 (MQ=255)                                                                                                                                                                        aCTTACCTTCTTCGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGGGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGAATACg                                >  1:594888/1‑251 (MQ=255)                                                                                                                                                                                tcttcGACTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGAATACGCCTGTAc                         <  1:101521/250‑1 (MQ=255)                                                                                                                                                                                      aCTAAATAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGcc                                                   >  1:347011/1‑218 (MQ=255)                                                                                                                                                                                           aTAGCCGTCACAATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGAATACGCCTGTACTGGCGGTTCttt             >  1:408298/1‑249 (MQ=255)                                                                                                                                                                                                     caATTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGt                                                                                                                                                                 >  1:376025/1‑93 (MQ=255)                                                                                                                                                                                                      aaTTCGGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACTAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGAATACGCCTGTACTGGCGGTTCTGATCGCCATATTg  <  1:834083/251‑1 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      AATCGGTTCTTCTTTCGGTGGCGCCACTTCGCCGTTTTTCAGAATTTTGCGCGTATTATCGCATTGTGTACACGCCATATACTTACCGAAGCGCCCCAGTTTAAGGTGCATATCACCGCCGCATTTATCGCATTCAACGATTGGGCCGTCATAACCTTTAATCTTAAACTTACCTTCTTCGACTAAATAGCCGTCACAATTCAGGTTATTGCCGCAAATATGTAGTTTTCGATGAGGATCGATAATATAACTGTCCATCGCCGTACCGCATTTCGGGCAGCGTTTACGTTCCATTAGCGCTTTGGTTTCCGACGCGTCGTCCAACACATTTAATAGTTCCGCTTCAGGAACCAGGTTAATGGTGGTTTTGCAACGTGCTTTCGGTGGTAAGGCATAGCCGGTGCAGCCTAAGAATACGCCTGTACTGGCGGTTCTGATCGCCATATTG  >  1/1466705‑1467152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑