Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,461,672	C→T	intergenic (+176/‑492)	unknown → / → unknown	FIG00698722: hypothetical protein/Bacterial non‑heme ferritin (EC 1.16.3.2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,461,672	0	C	T	100.0%	40.3 / NA	16	intergenic (+176/‑492)	unknown/unknown	FIG00698722: hypothetical protein/Bacterial non‑heme ferritin (EC 1.16.3.2)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

CAGCTATCCAATTTTATTAAGACGGATAAAGGCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACACTTCTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTTCATCACGTTTAACTAGTAATAGTGACCTTTGAATAACTCTGTGTTTTCATTAAATCGTAGTGTTCATTTACTTTGCCACTAATTTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGGTTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTC  >  1/1461458‑1461866                                                                                                                                                                                                                       |                                                                                                                                                                                                   aaatttcccgcccctgcaaatacccctaaagccacgtaataactgctgactagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatta                                                                                                                                                                <  1:358946‑M2/164‑1 (MQ=255)    tttcccgcccctgcaaatacccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGt                                                                                                                                                             <  1:641107‑M2/167‑1 (MQ=255)                    atacccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATgg                                                                                                                                             <  1:334688‑M2/183‑1 (MQ=255)                       cccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatt                                                                                                                                                                 <  1:332040‑M2/163‑1 (MQ=255)                       cccctaaagccacgtaataactgctgattagataatgcgatacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTaa                                                                                                                                          >  1:814500‑M2/66‑251 (MQ=255)                                             gctgATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatt                                                                                                                                                                 >  1:591573/1‑206 (MQ=255)                                                gATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACatatat                                                                                                                                                                  <  1:873395/202‑1 (MQ=255)                                                gATTAGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATAtatta                                                                                                                                                                <  1:882711/204‑1 (MQ=255)                                                    aGATAATGCGATACGTGCTTGAATTTATGCTTACATTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACatatat                                                                                                                                                                  >  1:413914/1‑198 (MQ=255)                                                              atacgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTaaaaa                                                                                                    <  1:732398‑M2/224‑1 (MQ=255)                                                                 cgtgcttgaatttatgcttacatTTTTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGa                                                                                                 >  1:684168‑M2/24‑250 (MQ=255)                                                                                                                atatTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGt                                                                                                                                                             >  1:796235/1‑143 (MQ=255)                                                                                                                             ttACAAGGCTTTATTTCTTCAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTaaaaa                                                                                                    >  1:661888/1‑187 (MQ=255)                                                                                                                                            tcttcAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTag                     <  1:352855/251‑1 (MQ=255)                                                                                                                                             cttcAGTTTCCATCACGTTTAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTaga                    >  1:313791/1‑251 (MQ=255)                                                                                                                                                               ttAACCAGTAAGAGTGACCTGTGAATAACTCTGTGTTTTTATTAATCCGTAGCGTTTATTAACGAAGTCACTAAATTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGATTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTtc  <  1:807161/251‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                   CAGCTATCCAATTTTATTAAGACGGATAAAGGCACGTAATAACTGCTGATTAGATAATGCGATACGTGCTTGAATTTATGCTTACACTTCTAAATAAGTCTTGATAAATCAATATTTATTGATGTTACAAGGCTTTATTTCTTCAGTTTTCATCACGTTTAACTAGTAATAGTGACCTTTGAATAACTCTGTGTTTTCATTAAATCGTAGTGTTCATTTACTTTGCCACTAATTTCGCATAACATATATTATGTTAAATAAAATAAATGGTAAGTCACTTCTGGGAATTTGAGGTTTGCTCTCTTTAAAAATGACTGGGTAAAAAAATTTATTTCTTGAAGATTTGATTTTTTGAGCGTTTATGATGATGAGATATTCGTGAGTTTTTAGAGGTAGGTGAAAATACTTC  >  1/1461458‑1461866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑