Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,458,866	G→A	intergenic (+103/‑145)	unknown → / → unknown	Exodeoxyribonuclease I (EC 3.1.11.1)/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,458,866	0	G	A	100.0%	85.0 / NA	32	intergenic (+103/‑145)	unknown/unknown	Exodeoxyribonuclease I (EC 3.1.11.1)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

CCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGA  >  1/1458628‑1459002                                                                                                                                                                                                                                               |                                                                                                                                         ccGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATc                                                                                                                               <  1:759570/250‑1 (MQ=255)                tGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAAt                                                                                                               <  1:511985/251‑1 (MQ=255)                                          gTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGt                                                                                      >  1:709458/1‑250 (MQ=255)                                           tCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTc                                                                                     <  1:839570/250‑1 (MQ=255)                                            cTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCAt                                                                                   <  1:882102/251‑1 (MQ=255)                                                  gAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGt                                                                                      <  1:41881/242‑1 (MQ=255)                                                            aaCAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCt                                                                    <  1:878696/250‑1 (MQ=255)                                                             aCAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCttt                                                                  >  1:513464/1‑251 (MQ=255)                                                              cAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATG‑ATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCg                                                                >  1:571705/1‑251 (MQ=255)                                                                atatTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGt                                                               >  1:302902/1‑251 (MQ=255)                                                                                    aTCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTgg                                                                            <  1:192390/218‑1 (MQ=255)                                                                                    aTCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                              <  1:122950/248‑1 (MQ=255)                                                                                    aTCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGt                                           >  1:775909/1‑251 (MQ=255)                                                                                                tGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATc                                     <  1:270491/245‑1 (MQ=255)                                                                                                   aaaaGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                            <  1:27890/251‑1 (MQ=255)                                                                                                      aGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAATTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                            >  1:587128/1‑248 (MQ=255)                                                                                                      aGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATaaa                         >  1:524451/1‑251 (MQ=255)                                                                                                        gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCGGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:437133/1‑250 (MQ=255)                                                                                                        gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCGGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:429091/1‑250 (MQ=255)                                                                                                        gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAAt                                                <  1:150816/226‑1 (MQ=255)                                                                                                        gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCt                                 <  1:583539/241‑1 (MQ=255)                                                                                                        gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:558549/1‑250 (MQ=255)                                                                                                        gTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAc                        >  1:752815/1‑250 (MQ=255)                                                                                                         tCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                       <  1:503538/250‑1 (MQ=255)                                                                                                         tCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                       <  1:864185/250‑1 (MQ=255)                                                                                                           tATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCt                     >  1:200100/1‑250 (MQ=255)                                                                                                                     gAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                            >  1:634811/1‑233 (MQ=255)                                                                                                                     gAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTaa          >  1:843898/1‑251 (MQ=255)                                                                                                                        cGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt       <  1:22249/251‑1 (MQ=255)                                                                                                                             ataatCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGa  <  1:235117/251‑1 (MQ=38)                                                                                                                                   ggTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                              >  1:303474/1‑201 (MQ=25)                                                                                                                                    gTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGAATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAAcc                       <  1:728771/223‑1 (MQ=25)                                                                                                                                                                                                                                               |                                                                                                                                         CCGTAAAAAATTAGATGCCGAGGCTTTGCAATTTGAACAACGTCTTCAAGAATTAGCCGAACAATATTCCAACAATGAAGAAAATCTTATGTTATTGCAAAAGGTCTATGAATATGGAACGAAAATAATCGGTTAAAAGTGATCTGCCCCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAGTGA  >  1/1458628‑1459002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑