Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,453,714	T→C	F610L (TTT→CTT)	unknown →	Chromosome partition protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,453,714	0	T	C	100.0%	179.5 / NA	63	F610L (TTT→CTT)	unknown	Chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/41);  total (22/41)

GCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCTTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCGCCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTC  >  1/1453470‑1453960                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGACTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGc                                                                                                                                                                                                                                                   >  1:85214/1‑250 (MQ=255)               cacaAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAAt                                                                                                                                                                                                                                    >  1:589041/1‑251 (MQ=255)                                   cAAACACTGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATg                                                                                                                                                                                                                    <  1:637258/247‑1 (MQ=255)                                   cAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAgg                                                                                                                                                                                                                 <  1:573869/250‑1 (MQ=255)                                                          gTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCAt                                                                                                                                                                                         >  1:260172/1‑251 (MQ=255)                                                           ttACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCAt                                                                                                                                                                                         <  1:868472/250‑1 (MQ=255)                                                               aTGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTc                                                                                                                                                                                    <  1:409071/251‑1 (MQ=255)                                                                  aaTTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTc                                                                                                                                                                                    <  1:803829/248‑1 (MQ=255)                                                                  aaTTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACg                                                                                                                                                                                 >  1:720930/1‑251 (MQ=255)                                                                  aaTTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACg                                                                                                                                                                                 >  1:156816/1‑251 (MQ=255)                                                                    ttGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGc                                                                                                                                                                               <  1:752326/251‑1 (MQ=255)                                                                             cGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAgcgg                                                                                                                                                                       >  1:810840/1‑250 (MQ=255)                                                                                    aTCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                               <  1:804601/251‑1 (MQ=255)                                                                                    aTCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                               >  1:772989/1‑251 (MQ=255)                                                                                      caacaacaaAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACg                                                                                                                                                             >  1:18531/1‑251 (MQ=255)                                                                                                gTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCATAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                               <  1:430278/239‑1 (MQ=255)                                                                                                     gTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAg                                                                                                                                                                                                                  >  1:277310/1‑183 (MQ=255)                                                                                                     gTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGcc                                                                                                                                                                                                         <  1:65376/192‑1 (MQ=255)                                                                                                      tCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAg                                                                                                                                              <  1:289809/250‑1 (MQ=255)                                                                                                       cAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCg                                                                                                                                            >  1:897482/1‑251 (MQ=255)                                                                                                          attattAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCgg                                                                                                                                           <  1:581563/249‑1 (MQ=255)                                                                                                           ttattaAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTg                                                                                                                                                         <  1:845995/234‑1 (MQ=255)                                                                                                                     gttttAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAGAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGc                                                                                                                               >  1:428306/2‑250 (MQ=255)                                                                                                                     aTTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGaa                                                                                                                                                               >  1:384106/1‑218 (MQ=255)                                                                                                                     aTTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGc                                                                                                                                             >  1:593846/1‑236 (MQ=255)                                                                                                                                  cgcCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCg                                                                                                                 <  1:174084/251‑1 (MQ=255)                                                                                                                                       aTTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGtgatg                                                                                                            <  1:639464/251‑1 (MQ=255)                                                                                                                                          tGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAAt                                                                                                         >  1:421559/1‑251 (MQ=255)                                                                                                                                                   aaaCCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGc                                                                                                  <  1:241748/249‑1 (MQ=255)                                                                                                                                                               aaTTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAgcggcg                                                                                                                                                                     <  1:195682/170‑1 (MQ=255)                                                                                                                                                               aaTTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGtt                                                                                    <  1:577406/251‑1 (MQ=255)                                                                                                                                                                     aagaaTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAagag                                                                              <  1:28656/251‑1 (MQ=255)                                                                                                                                                                     aagaaTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                <  1:652047/249‑1 (MQ=255)                                                                                                                                                                         aTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAaga                                                                               >  1:512392/1‑246 (MQ=255)                                                                                                                                                                         aTTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTg                                                                          >  1:758395/1‑251 (MQ=255)                                                                                                                                                                              aTGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                >  1:198749/1‑240 (MQ=255)                                                                                                                                                                              aTGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTc                                                                     <  1:121115/251‑1 (MQ=255)                                                                                                                                                                                         gAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATgg                                                              <  1:550733/247‑1 (MQ=255)                                                                                                                                                                                             cTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGAc                                                                                                                           <  1:92846/182‑1 (MQ=255)                                                                                                                                                                                                  aTTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAag                                                                                <  1:614777/220‑1 (MQ=255)                                                                                                                                                                                                         aTTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGtt                                           <  1:274138/250‑1 (MQ=255)                                                                                                                                                                                                         aTTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTAAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGtt                                           <  1:817125/250‑1 (MQ=255)                                                                                                                                                                                                                     aaCTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCaa                              <  1:204086/251‑1 (MQ=255)                                                                                                                                                                                                                     aaCTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCaa                              <  1:377766/251‑1 (MQ=255)                                                                                                                                                                                                                       cTTTCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAAtt                            >  1:554712/1‑251 (MQ=255)                                                                                                                                                                                                                         ttCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATC‑AAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                         <  1:461648/251‑1 (MQ=255)                                                                                                                                                                                                                          tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGGAGTTTTTGAGGAGAGCGCCCGTAAAGCACCGGCATGGCTCACGGCACATGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGTCAAGGCGTGATGAATTTCATGCAGGCACAGGTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGGTACAACGTCAGCAATTAGa                         <  1:257693/251‑1 (MQ=255)                                                                                                                                                                                                                          tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                         <  1:419793/251‑1 (MQ=255)                                                                                                                                                                                                                          tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                         <  1:200895/251‑1 (MQ=255)                                                                                                                                                                                                                          tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                         <  1:386418/251‑1 (MQ=255)                                                                                                                                                                                                                          tCGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGAATGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGa                         <  1:106295/251‑1 (MQ=255)                                                                                                                                                                                                                           cGGACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGAt                        <  1:585995/251‑1 (MQ=255)                                                                                                                                                                                                                             gACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATg                       <  1:754129/250‑1 (MQ=255)                                                                                                                                                                                                                             gACTTGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGa                      >  1:138095/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGGTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGt                                            >  1:840435/1‑225 (MQ=255)                                                                                                                                                                                                                                   gTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAg                                             <  1:861633/222‑1 (MQ=255)                                                                                                                                                                                                                                   gTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACg                                     <  1:85962/230‑1 (MQ=255)                                                                                                                                                                                                                                   gTAGTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAAt                >  1:771076/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTGCAGCGTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAgg                                                                                                                                                                                                                 <  1:552246/55‑1 (MQ=255)                                                                                                                                                                                                                                             gTTCAACCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTa      >  1:658467/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aaCCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTc  <  1:274876/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaCCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTc  <  1:796506/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaCCCTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCACCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTc  <  1:877187/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GCGTGAATATCCGACACAAAAACTGCAAGCACAGCAAACACCGCAACTACGCGCCAAGTTACATGAATTGGAACAACGTCTGAATCAACAACAAAGTGCGGTCAGATTATTAAACGATTTTAATCAACGCGCCAATTTGTCTTTAGAAACCGCAGATGAATTAGAAGAATTTTATGGTGAACAGGAAGCTTTAATTGAGGATTTGTCGGCTGAACTTTCGGACTTGGTAGTGCAGCGTTCAACCTTTCGCCAAAAACGTGAAAATTTGACCGCACTTTATGAGGAAAACGCCCGTAAAGCGCCGGCATGGCTCACGGCACAAGCGGCGTTGGAACGATTGCAGGATCAAAGCGGTGAGCAATTTGCCGACAGCCAAGGCGTGATGAATTTCATGCAGGCACAGTTGGTTAAAGAGCGTGAATTCACCATGGAGCGCGATCAGCTTGAGTTACAACGTCAGCAATTAGATGAGCAAATCAGCCGATTAAGTC  >  1/1453470‑1453960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑