Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,446,705	T→G	I184S (ATC→AGC)	unknown →	Chromosome partition protein MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,446,705	0	T	G	100.0%	180.4 / NA	60	I184S (ATC→AGC)	unknown	Chromosome partition protein MukF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/35);  total (25/35)

ATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGATCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGACCGCATTATCAGTT  >  1/1446497‑1446950                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      aTTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGCTTATTAAcc                                                                                                                                                                                                                        <  1:409182/240‑1 (MQ=255)    cGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAAc                                                                                                                                                                                                                         <  1:535239/236‑1 (MQ=255)                 tttCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGt                                                                                                                                                                                             <  1:901268/251‑1 (MQ=255)                   tCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTtg                                                                                                                                                                                           <  1:901707/251‑1 (MQ=255)                        cTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAAc                                                                                                                                                                                      <  1:257159/251‑1 (MQ=255)                           gcgcCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAat                                                                                                                                                                                   >  1:872693/1‑251 (MQ=255)                                tttCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAat                                                                                                                                                                                   <  1:214727/246‑1 (MQ=255)                                tttCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAAttatta                                                                                                                                                                              >  1:312609/1‑251 (MQ=255)                                                         cGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACggg                                                                                                                                                     <  1:687164/251‑1 (MQ=255)                                                          gATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAAcc                                                                                                                                                                                                                        <  1:337633/183‑1 (MQ=255)                                                                    aaCGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAGGAt                                                                                                                                          <  1:877256/251‑1 (MQ=255)                                                                    aaCGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGAt                                                                                                                                          <  1:656042/251‑1 (MQ=255)                                                                     aCGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGAt                                                                                                                                          <  1:296734/250‑1 (MQ=255)                                                                      cGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATAc                                                                                                                                        <  1:741771/251‑1 (MQ=255)                                                                          ccTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATAccc                                                                                                                                      <  1:146545/249‑1 (MQ=255)                                                                                     gccgAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGc                                                                                                                                                               <  1:172597/213‑1 (MQ=255)                                                                                           gaagaTGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGa                                                                                                                                                                                                                                              <  1:89288/128‑1 (MQ=255)                                                                                                          cATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGAt                                                                                                                                          >  1:176058/1‑213 (MQ=255)                                                                                                          cATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAatt                                                                                                    >  1:787453/1‑251 (MQ=255)                                                                                                          cATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAatt                                                                                                    >  1:365764/1‑251 (MQ=255)                                                                                                            ttattGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAg                                                                                                                                                                                              <  1:404218/159‑1 (MQ=255)                                                                                                            ttattGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAattat                                                                                                  >  1:215597/1‑251 (MQ=255)                                                                                                                      cgtAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTg                                                                                                <  1:309122/243‑1 (MQ=255)                                                                                                                             tCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTg                                                                                  >  1:509408/1‑250 (MQ=255)                                                                                                                                     ccGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCgg                                                                         >  1:464522/1‑251 (MQ=255)                                                                                                                                     ccGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCg                                                                          <  1:247293/250‑1 (MQ=255)                                                                                                                                      cGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCggg                                                                        <  1:51129/251‑1 (MQ=255)                                                                                                                                       gTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTtg                                                                                                                                                                                           <  1:315111/135‑1 (MQ=255)                                                                                                                                       gTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCggg                                                                        >  1:247726/1‑250 (MQ=255)                                                                                                                                       gTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCggg                                                                        >  1:132867/1‑250 (MQ=255)                                                                                                                                       gTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCggg                                                                        >  1:556800/1‑250 (MQ=255)                                                                                                                                       gTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCg                                                                          >  1:395149/1‑248 (MQ=255)                                                                                                                                          aaaaTATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGAt                                                                                                                                          >  1:753069/1‑181 (MQ=255)                                                                                                                                          aaaaTATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGcgcg                                                                    >  1:250885/1‑251 (MQ=255)                                                                                                                                          aaaaTATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGACAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGAAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGcgcg                                                                    >  1:456084/1‑251 (MQ=255)                                                                                                                                                     tGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTAt                                                         <  1:617802/251‑1 (MQ=255)                                                                                                                                                           aaaTTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTAGTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAGATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTAt                                                    <  1:442295/250‑1 (MQ=255)                                                                                                                                                                      aGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTAt                                                    <  1:843527/239‑1 (MQ=255)                                                                                                                                                                       gCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTAtttt                                                      <  1:329771/236‑1 (MQ=255)                                                                                                                                                                             aCTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATc                                                                           <  1:363057/209‑1 (MQ=255)                                                                                                                                                                             aCTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTAt                                                    >  1:825008/1‑232 (MQ=255)                                                                                                                                                                             aCTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAAt                                 >  1:385726/1‑251 (MQ=255)                                                                                                                                                                             aCTTATCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAAt                                 >  1:328506/1‑251 (MQ=255)                                                                                                                                                                                 aTCGCAACGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTac                             <  1:830401/251‑1 (MQ=255)                                                                                                                                                                                       aCGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCg                                                                          >  1:293433/1‑200 (MQ=255)                                                                                                                                                                                       aCGTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTacaaacaa                       >  1:568334/1‑251 (MQ=255)                                                                                                                                                                                         gTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTg                                                                                                <  1:180614/176‑1 (MQ=255)                                                                                                                                                                                         gTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCg                                                                          <  1:664266/198‑1 (MQ=255)                                                                                                                                                                                         gTATTATGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACaaaa                     >  1:492852/1‑251 (MQ=255)                                                                                                                                                                                           attatGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCATGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTac                             <  1:313788/241‑1 (MQ=255)                                                                                                                                                                                           attatGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAatt                                                                                                    >  1:713144/1‑170 (MQ=255)                                                                                                                                                                                             tatGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCGGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAg                 <  1:96501/251‑1 (MQ=255)                                                                                                                                                                                               tGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACTAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGAc               <  1:898732/251‑1 (MQ=255)                                                                                                                                                                                               tGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGAc               <  1:819604/251‑1 (MQ=255)                                                                                                                                                                                               tGGACGAAAACCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGAc               <  1:524682/251‑1 (MQ=255)                                                                                                                                                                                                     aaaaCCAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTAt                                                    >  1:38288/1‑208 (MQ=255)                                                                                                                                                                                                         ccAGCAGAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGACCGCATTATCa     <  1:719940/251‑1 (MQ=255)                                                                                                                                                                                                            gcagAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCaa                                  <  1:332392/219‑1 (MQ=255)                                                                                                                                                                                                            gcagAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGACCGCATTATCAGtt  >  1:364824/1‑251 (MQ=255)                                                                                                                                                                                                            gcagAGCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGACCGCATTATCAGtt  >  1:250531/1‑251 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ATTCGTCAACGCGAATTTTCCGCCTTGCGCCTTTCCGTTCAGCTTTCCATTGTCGCCGATGAAATTCAACGTGCCTCTGACGCCGCCGAAGAAGATGGCGACGAACATTATTGGCGTCGTAACGTCTTTGCGCCGTTAAAATATTCCGTGGCAGAAATTTTCGACAGCATCGACTTATCGCAACGTATTATGGACGAAAACCAGCAGATCATTAAGGACGAAATTGCCGGTTTATTAACCAAAGATTGGCAGGCGGCGATTTCCAGTTGTGAACAATTATTAGACGAAACCTCAGGCAATTTACGGGAACTACAAGATACCCTTAACGCCGCCGGCGACAAATTGCAGGCGCAATTATTGCGGATTCAGGACTGCGTTATCGGGCGCGGTGAACTGTATTTTATTGATGAGCTGATCACCAATTTACAAACAAAACTAGACCGCATTATCAGTT  >  1/1446497‑1446950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑