Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,442,716	A→C	L737F (TTA→TTC)	unknown →	Glycogen phosphorylase (EC 2.4.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,442,716	0	A	C	100.0%	199.8 / NA	67	L737F (TTA→TTC)	unknown	Glycogen phosphorylase (EC 2.4.1.1)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/31);  total (36/31)

GGCGGGTACGGAAGCCTCCGGTACCGGTAACATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCGTCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTATCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGA  >  1/1442470‑1442949                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ggCGGGTACGGAAGCCTCCGGTACCGGTAACATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCa                                                                                                                                                                                                                                        >  1:380104/1‑250 (MQ=255)  gCGGGTACGGAAGCCTCCGGTACCGGTAACATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCAc                                                                                                                                                                                                                                       <  1:31977/250‑1 (MQ=255)     ggTACGGAAGCCTCCGGTACCGGTAACATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGaa                                                                                                                                                                                                                                   >  1:628400/1‑251 (MQ=255)       tACGGAAGCCTCCGGTACCGGTAACATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATg                                                                                                                                                                                                                                 <  1:435919/251‑1 (MQ=255)                             aaCATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAAtt                                                                                                                                                                                                           >  1:757448/1‑251 (MQ=255)                               cATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGt                                                                                                                                                                                                         >  1:280436/1‑251 (MQ=255)                                      tttGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAggg                                                                                                                                                                                                  <  1:2362/251‑1 (MQ=255)                                      tttGCCCTTAACGGCGCCTTAATATTAGGTACGTT‑GACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAggg                                                                                                                                                                                                  <  1:77402/250‑1 (MQ=255)                                         gCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATATTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGt                                                                                                                                                                                                 >  1:183834/1‑249 (MQ=255)                                            cTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTg                                                                                                                                                                                                          >  1:321071/1‑237 (MQ=255)                                              tAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAat                                                                                                                                                                                          <  1:577599/251‑1 (MQ=255)                                              tAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAat                                                                                                                                                                                          <  1:496989/251‑1 (MQ=255)                                                aCGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAatat                                                                                                                                                                                        <  1:616025/251‑1 (MQ=255)                                                aCGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAatat                                                                                                                                                                                        <  1:511088/251‑1 (MQ=255)                                                      ccTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAAt                                                                                                                                                                                                                                  <  1:254932/203‑1 (MQ=255)                                                      ccTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAatat                                                                                                                                                                                        >  1:854511/1‑245 (MQ=255)                                                      ccTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGa                                                                                                                                                                                   >  1:185520/1‑250 (MQ=255)                                                      ccTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGa                                                                                                                                                                                   >  1:179084/1‑250 (MQ=255)                                                          aaTATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTa                                                                                                                                                                                                                    <  1:819654/213‑1 (MQ=255)                                                           atatTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAat                                                                                                                                                                                          <  1:900437/238‑1 (MQ=255)                                                             atTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATc                                                                                                                                                                           <  1:47849/251‑1 (MQ=255)                                                             atTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATc                                                                                                                                                                           <  1:384507/251‑1 (MQ=255)                                                                  gTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGAcc                                                                                                                                                                                                                              <  1:24602/195‑1 (MQ=255)                                                                  gTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAgg                                                                                                                                                                        <  1:689613/249‑1 (MQ=255)                                                                    aCGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGa                                                                                                                                                                                   >  1:81835/1‑236 (MQ=255)                                                                    aCGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCAtt                                                                                                                                                                    >  1:690159/1‑251 (MQ=255)                                                                    aCGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCAtt                                                                                                                                                                    >  1:93420/1‑251 (MQ=255)                                                                      gTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGc                                                                                                                                                                                                               <  1:534111/206‑1 (MQ=255)                                                                              gcgcAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCa                                                                                                                                                                          >  1:891061/1‑235 (MQ=255)                                                                                           aaTTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTgg                                                                                                                                             <  1:302754/251‑1 (MQ=255)                                                                                           aaTTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTgg                                                                                                                                             <  1:289165/251‑1 (MQ=255)                                                                                                       tGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAg                                                                                                                                 <  1:165321/251‑1 (MQ=255)                                                                                                           ggTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAgtggt                                                                                                                             >  1:134081/1‑251 (MQ=255)                                                                                                            gTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAgtggtg                                                                                                                            >  1:792801/1‑251 (MQ=255)                                                                                                                aGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGAGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACg                                                                                                                        >  1:803518/1‑251 (MQ=255)                                                                                                                 gACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACga                                                                                                                       >  1:665523/1‑251 (MQ=255)                                                                                                                  acaacaTCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACg                                                                                                                        <  1:397548/249‑1 (MQ=255)                                                                                                                              ttttCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAAt                                                                                                          <  1:439003/251‑1 (MQ=255)                                                                                                                                  cGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCgag                                                                                                      >  1:642164/1‑251 (MQ=255)                                                                                                                                    gTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGTAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGAt                                                                                                    >  1:612433/1‑251 (MQ=255)                                                                                                                                          ccGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATaaaaa                                                                                                           >  1:585031/1‑238 (MQ=255)                                                                                                                                          ccGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTgg                                                                                              >  1:187212/1‑251 (MQ=255)                                                                                                                                          ccGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTgg                                                                                              >  1:749763/1‑251 (MQ=255)                                                                                                                                                        gcgcAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAg                                                                                                                                 <  1:615630/202‑1 (MQ=255)                                                                                                                                                           cAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTgg                                                                                                                                             >  1:639820/1‑187 (MQ=255)                                                                                                                                                           cAGTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCAt                                                                                >  1:93142/1‑248 (MQ=255)                                                                                                                                                             gTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAg                                                                                      >  1:304426/1‑240 (MQ=255)                                                                                                                                                             gTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCaaa                                                                           >  1:494283/1‑251 (MQ=255)                                                                                                                                                             gTTACGCCGTGACGGTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCaaa                                                                           >  1:755326/1‑251 (MQ=255)                                                                                                                                                                           gTTATCATCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCg                                                                                                                                                                                                                                     <  1:664580/83‑1 (MQ=255)                                                                                                                                                                                catCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTAGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTa                                                        <  1:851492/249‑1 (MQ=255)                                                                                                                                                                                    cGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTtc                                                    <  1:143971/251‑1 (MQ=255)                                                                                                                                                                                     gTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAAt                                                                                                          <  1:866909/196‑1 (MQ=255)                                                                                                                                                                                     gTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTAttt                                                     >  1:631281/1‑249 (MQ=255)                                                                                                                                                                                             attatCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTcggac                                           <  1:226752/251‑1 (MQ=255)                                                                                                                                                                                               tatCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACAt                                                              >  1:550278/1‑230 (MQ=255)                                                                                                                                                                                                             gcactttcacggccacttctttttcatcatcAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAat                           >  1:392088/26‑251 (MQ=255)                                                                                                                                                                                                              gAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATc                                                                                                                                                                           <  1:361931/106‑1 (MQ=255)                                                                                                                                                                                                              gAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGaa                            >  1:758358/1‑249 (MQ=255)                                                                                                                                                                                                                aTTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGta                                                                                                                 >  1:56610/1‑162 (MQ=255)                                                                                                                                                                                                                aTTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAAt                                                                                                          <  1:751993/169‑1 (MQ=255)                                                                                                                                                                                                                          gtggtTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAatat              >  1:306720/1‑251 (MQ=255)                                                                                                                                                                                                                           tggtTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATc             <  1:334495/251‑1 (MQ=255)                                                                                                                                                                                                                           tggtTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATc             <  1:93123/251‑1 (MQ=255)                                                                                                                                                                                                                            ggtTGATCAAATCATCAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAgg                              >  1:805520/1‑233 (MQ=255)                                                                                                                                                                                                                                      atcatcAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAAt                                                                                                          >  1:571846/1‑147 (MQ=255)                                                                                                                                                                                                                                      atcatcAACGGCGCGTTCTCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGa  >  1:893701/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          GGCGGGTACGGAAGCCTCCGGTACCGGTAACATGAAGTTTGCCCTTAACGGCGCCTTAATATTAGGTACGTTGGACGGCGCAAACGTGGAAATTCTGGAAAATGTCGGTAAAGACAACATCTTCATTTTCGGTAACACCGTTGAGCAAGTAGCGCAGTTACGCCGTGACGGTTATCGTCCGTTTGATTATTATCAAAATGACGCGGAATTAAGAACGGTGGTTGATCAAATCATCAACGGCGCGTTATCACCGAATGACCCGAATCGCTATCTGCAATTGTTACAGGGTCTCCAATATCACGACTATTATCAGGCATTCGCCGATTTCCGCAGTTATGTGGATATGCAAAAAGTGGTGGACGAGAAGTATAAAAATCGAGATGCGTGGATTGAAAGCACCATTCAAAACATCGTCAACATGGGCTATTTCTCCTCGGACCGGACGATTAAGGAATATGCGGAAAATATCTGGAATATTGA  >  1/1442470‑1442949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑