Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,438,352	C→T	G307G (GGC→GGT)	unknown →	Glucose‑1‑phosphate adenylyltransferase (EC 2.7.7.27)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,438,352	0	C	T	100.0%	217.4 / NA	77	G307G (GGC→GGT)	unknown	Glucose‑1‑phosphate adenylyltransferase (EC 2.7.7.27)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/44);  total (33/44)

GAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTCGATATTTACGACCAAACCTGGCCGATTCGTGGCAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTATCGATCGCCACTGTATTAT  >  1/1438109‑1438594                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   gAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCgg                                                                                                                                                                                                                                             >  1:593244/1‑251 (MQ=255)   aTCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTg                                                                                                                                                                                                                                           >  1:34421/1‑251 (MQ=255)       ggAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAg                                                                                                                                                                                                                                       >  1:761009/1‑251 (MQ=255)                 acGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATc                                                                                                                                                                                                                             <  1:373769/251‑1 (MQ=255)                   gCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATcc                                                                                                                                                                                                                                <  1:417352/246‑1 (MQ=255)                      ttGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAAt                                                                                                                                                                                                                        <  1:568340/251‑1 (MQ=255)                             tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATcc                                                                                                                                                                                                                                               <  1:881705/221‑1 (MQ=255)                             tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATcc                                                                                                                                                                                                                                <  1:820426/236‑1 (MQ=255)                             tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTta                                                                                                                                                                                                                 >  1:271088/1‑251 (MQ=255)                             tctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTta                                                                                                                                                                                                                 >  1:269452/1‑251 (MQ=255)                              ctcACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTtat                                                                                                                                                                                                                >  1:691597/1‑251 (MQ=255)                                    aCTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATaaaaa                                                                                                                                                                                                           >  1:862850/1‑250 (MQ=255)                                         ggTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCaa                                                                                                                                                                                                     >  1:438632/1‑251 (MQ=255)                                            aaaGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAatat                                                                                                                                                                                                  <  1:455437/251‑1 (MQ=255)                                                 cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                                                             <  1:844517/251‑1 (MQ=255)                                                 cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                                                             <  1:700332/251‑1 (MQ=255)                                                 cGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATAGCAAAc                                                                                                                                                                                             <  1:268593/251‑1 (MQ=255)                                                    aTTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCgg                                                                                                                                                                                          <  1:747721/251‑1 (MQ=255)                                                         gcatttcatagagataatcggcgTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGcc                                                                                                                                                                                                                                     <  1:801907/183‑1 (MQ=255)                                                               gTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCttt                                                                                                                                                                                                                   <  1:872258/215‑1 (MQ=255)                                                               gTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAAct                                                                                                                                                                                 >  1:84025/1‑249 (MQ=255)                                                                        aagGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                                                       <  1:877786/250‑1 (MQ=255)                                                                               gTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCttt                                                                                                                                                                                                                   <  1:33752/199‑1 (MQ=255)                                                                                ttATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                                                             >  1:844309/1‑220 (MQ=255)                                                                                ttATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTa                                                                                                                                                                           <  1:767300/238‑1 (MQ=255)                                                                                  ataCGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCg                                                                                                                                                            <  1:271412/251‑1 (MQ=255)                                                                                           cccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCg                                                                                                                                                   >  1:745837/1‑251 (MQ=255)                                                                                           cccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCg                                                                                                                                                   >  1:446270/1‑251 (MQ=255)                                                                                            ccGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                                                       <  1:9265/230‑1 (MQ=255)                                                                                                       cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCatcaatc                                                                                                                                        <  1:556012/250‑1 (MQ=255)                                                                                                       cTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCatcaa                                                                                                                                          <  1:68321/248‑1 (MQ=255)                                                                                                            gCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGt                                                                                                                                                                      >  1:106719/1‑215 (MQ=255)                                                                                                            gCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTAt                                                                                                                                  >  1:469734/1‑251 (MQ=255)                                                                                                              aTGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                                                <  1:649289/251‑1 (MQ=255)                                                                                                                 ggACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCg                                                                                                                              <  1:636167/250‑1 (MQ=255)                                                                                                                   aCGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                                                >  1:474069/1‑246 (MQ=255)                                                                                                                     gTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGtgttg                                                                                                                         >  1:643327/1‑251 (MQ=255)                                                                                                                          aCCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCgg                                                                                                                                                                       <  1:833560/200‑1 (MQ=255)                                                                                                                               aGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCa                                                                                                               >  1:851673/1‑251 (MQ=255)                                                                                                                                      atTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCgtgt                                                                                                                           >  1:91418/1‑232 (MQ=255)                                                                                                                                        ttACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTa                                                                                                      >  1:101715/1‑251 (MQ=255)                                                                                                                                             ggCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCat                                                                                                                                             <  1:175970/207‑1 (MQ=255)                                                                                                                                                     gTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCa                                                                                         >  1:554853/1‑251 (MQ=255)                                                                                                                                                      tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACATCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAAt                                                                                                        <  1:803490/235‑1 (MQ=255)                                                                                                                                                      tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCg                                                                                                                 <  1:572413/226‑1 (MQ=255)                                                                                                                                                      tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                                                        >  1:583327/1‑251 (MQ=255)                                                                                                                                                      tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                                                        >  1:273311/1‑251 (MQ=255)                                                                                                                                                      tAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCaa                                                                                        >  1:543034/1‑251 (MQ=255)                                                                                                                                                        ggTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCGAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGAc                                                                                                                   <  1:84044/222‑1 (MQ=255)                                                                                                                                                        ggTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGATGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAAt                                                                                      >  1:456311/1‑251 (MQ=255)                                                                                                                                                           aCATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAgg                                                                                               <  1:395093/239‑1 (MQ=255)                                                                                                                                                                ggACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGAt                                                                                                                                                 <  1:374450/184‑1 (MQ=255)                                                                                                                                                                 gACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCAtt                                                                              >  1:351639/1‑250 (MQ=255)                                                                                                                                                                     gCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGt                                                                                                                                                                      >  1:448199/1‑158 (MQ=255)                                                                                                                                                                      cTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCg                                                                           <  1:893525/248‑1 (MQ=255)                                                                                                                                                                        tCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCgtggtg                                                                      <  1:682402/251‑1 (MQ=255)                                                                                                                                                                              aaTCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCg                                                                <  1:409862/251‑1 (MQ=255)                                                                                                                                                                               aTCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCg                                                                >  1:137141/1‑250 (MQ=255)                                                                                                                                                                                    atatCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAAt                                                                                      >  1:88344/1‑223 (MQ=255)                                                                                                                                                                                       tCGATTTGGTTTCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCTATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGaa                                                       <  1:432007/251‑1 (MQ=255)                                                                                                                                                                                                 ttCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTc                                                                                                                                <  1:339580/168‑1 (MQ=255)                                                                                                                                                                                                 ttCCGAAAGTCCGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAAt                                                                                                        <  1:389419/192‑1 (MQ=255)                                                                                                                                                                                                           ccGCAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGtt                                   >  1:546363/1‑251 (MQ=255)                                                                                                                                                                                                              cAATTGGATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAac                                <  1:135524/251‑1 (MQ=255)                                                                                                                                                                                                                attggATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCGAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAaca                               >  1:725826/5‑250 (MQ=255)                                                                                                                                                                                                                attggATATTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAacac                              <  1:330963/247‑1 (MQ=255)                                                                                                                                                                                                                     atatTTACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTg                          <  1:125257/250‑1 (MQ=255)                                                                                                                                                                                                                        tttACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAAc                                                                                                                                                                                             <  1:593467/84‑1 (MQ=255)                                                                                                                                                                                                                          tACGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGtattat                    <  1:678170/251‑1 (MQ=255)                                                                                                                                                                                                                            cGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTatcg                  <  1:217515/251‑1 (MQ=255)                                                                                                                                                                                                                            cGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTatcg                  <  1:609742/251‑1 (MQ=255)                                                                                                                                                                                                                            cGACCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTatcg                  <  1:521235/251‑1 (MQ=255)                                                                                                                                                                                                                              aCCAAACCTGGCCGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTatcgat                >  1:555529/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTATCGATCGCCACTGtat     <  1:474219/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccGATTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTATCGATCGCCACTGtat     <  1:485546/251‑1 (MQ=255)                                                                                                                                                                                                                                            aTTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAacac                              >  1:749819/1‑223 (MQ=255)                                                                                                                                                                                                                                            aTTCGTGGTAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTATCGATCGCCACTGtattat  >  1:358922/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   GAATCGGGAAGTCAACACGCCTTGCACCTCTCACGACTTTGGTAAAGACGTATTGCCGAAATGTCTGGAAGAAGGCGCGTTATACGCTCACCCGTTCAGCCGCTCCTGCATGGGACGTAATACCGAAGGCGATATTTACTGGCGTGACGTAGGTACATTGGACAGCTTCTGGCAATCCAATATCGATTTGGTTTCCGAAAGTCCGCAATTCGATATTTACGACCAAACCTGGCCGATTCGTGGCAATCCGGTGCAAGCCTATCCATCTAAATTCTTTTATAAAAACACCAATATCAAACCGGTGGATAACTCTCTTATCGGTGGCGGCTGCGTGATTACCGATGCATCAATCAGTTATTCCGTGTTGTTTGACCGCATCAAAATTAACGAAGGCTCGCAAATCGATCATTCCGTGGTGTTACCGCAAGTGGAAATCGGGAAAAACTGCGTGTTAACACATTGTATTATCGATCGCCACTGTATTAT  >  1/1438109‑1438594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑