Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,431,000 T→C G217G (GGT→GGC)  unknown → Glutaminyl‑tRNA synthetase (EC 6.1.1.18)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,431,0000TC98.6% 193.7 / ‑5.2 70G217G (GGT→GGC) unknownGlutaminyl‑tRNA synthetase (EC 6.1.1.18)
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (25/44);  total (25/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-01

TGGATGAACTTTCGCCGGAAGAAATGCGCGAATATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGTGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGAAGGCACCTTAACCTCTAAACGCAAACTCTTAAAATTGGTGAATGATGAAAT  >  1/1430756‑1431239
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
tgGATGAACTTTCGCCGGAAGAAATGCGCGAATATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTAATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACaaa                                                                                                                                                                                                                                           <  1:376746/251‑1 (MQ=255)
           tCGCCGGAAGAAATGCGCGAATATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTa                                                                                                                                                                                                                                <  1:409325/251‑1 (MQ=255)
              ggctcggaaaggtgtataagagacagCACCTTACCCGAACCGGGT‑AAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATcc                                                                                                                                                                                                                             <  1:301691/225‑1 (MQ=255)
              ccGGAAGAAATGCGCGAATATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATcc                                                                                                                                                                                                                             <  1:877944/251‑1 (MQ=255)
                 gaagaaATGCGCGAATATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGa                                                                                                                                                                                                                           <  1:344151/250‑1 (MQ=255)
                            cgAATATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTc                                                                                                                                                                                                                <  1:241692/250‑1 (MQ=255)
                              aaTATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCa                                                                                                                                                                                                               >  1:628351/1‑249 (MQ=255)
                              aaTATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCAc                                                                                                                                                                                                              <  1:353915/250‑1 (MQ=255)
                               atatCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTc                                                                                                                                                                                                            <  1:517353/251‑1 (MQ=255)
                                 atCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACaaa                                                                                                                                                                                                                                           >  1:244359/1‑218 (MQ=255)
                                     cgGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGTGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTa                                                                                                                                                                                                      <  1:77472/251‑1 (MQ=255)
                                        cACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTAtt                                                                                                                                                                                                    <  1:509567/250‑1 (MQ=255)
                                        cACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTAtt                                                                                                                                                                                                    <  1:248661/250‑1 (MQ=255)
                                          ccTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTc                                                                                                                                                                                                            >  1:259/1‑240 (MQ=255)
                                             tAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGAt                                                                                                                                                                                              >  1:183865/1‑251 (MQ=255)
                                             tAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGCAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCACCGTTTATGGAGATGCGCGATCCGATGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGAt                                                                                                                                                                                              >  1:552902/1‑251 (MQ=255)
                                                       gggTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTAtt                                                                                                                                                                                                    >  1:300470/1‑235 (MQ=255)
                                                        ggTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGTCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCg                                                                                                                                                                                   >  1:560029/1‑251 (MQ=255)
                                                        ggTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATcc                                                                                                                                                                                                                             <  1:652316/209‑1 (MQ=255)
                                                        ggTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTc                                                                                                                                                                                                  <  1:168516/236‑1 (MQ=255)
                                                        ggTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCg                                                                                                                                                                                   >  1:692736/1‑251 (MQ=255)
                                                                  gCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGTGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCa                                                                                                                                                                          >  1:736573/1‑250 (MQ=255)
                                                                  gCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTc                                                                                                                                                                                                  <  1:177183/226‑1 (MQ=255)
                                                                   cccATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGc                                                                                                                                                                           <  1:756804/248‑1 (MQ=255)
                                                                   cccATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCAc                                                                                                                                                                         <  1:178772/250‑1 (MQ=255)
                                                                       tatCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCtt                                                                                                                                                                    >  1:331786/1‑251 (MQ=255)
                                                                        atCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTa                                                                                                                                                                   >  1:134405/1‑251 (MQ=255)
                                                                         tCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTAt                                                                                                                                                                  <  1:464949/251‑1 (MQ=255)
                                                                          cGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACaaa                                                                                                                                                                                                                                           >  1:230202/1‑177 (MQ=255)
                                                                          cGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATg                                                                                                                                                                 <  1:880200/251‑1 (MQ=255)
                                                                          cGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATg                                                                                                                                                                 <  1:34923/251‑1 (MQ=255)
                                                                          cGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATg                                                                                                                                                                 <  1:330932/251‑1 (MQ=255)
                                                                          cGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATg                                                                                                                                                                 <  1:701362/251‑1 (MQ=255)
                                                                          cGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATg                                                                                                                                                                 <  1:557339/251‑1 (MQ=255)
                                                                           gTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATg                                                                                                                                                                 <  1:326593/250‑1 (MQ=255)
                                                                            tGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTa                                                                                                                                                               <  1:392944/251‑1 (MQ=255)
                                                                              aTCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAGGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTAc                                                                                                                                                              <  1:105926/250‑1 (MQ=255)
                                                                              aTCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTAc                                                                                                                                                              <  1:499678/250‑1 (MQ=255)
                                                                               tCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTg                                                                                                                                                                                                        <  1:569177/207‑1 (MQ=255)
                                                                               tCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTAcc                                                                                                                                                             >  1:199850/1‑250 (MQ=255)
                                                                                  cAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTAc                                                                                                                                                              <  1:659569/246‑1 (MQ=255)
                                                                                    gCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTc                                                                                                                                                                                                  <  1:400535/208‑1 (MQ=255)
                                                                                     cGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAAt                                                                                                                                                      <  1:274290/251‑1 (MQ=255)
                                                                                         gaagaaAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAAt                                                                                                                                                                                                                                          >  1:451656/1‑163 (MQ=255)
                                                                                          aagaaAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCaa                                                                                                                                                 >  1:454956/1‑251 (MQ=255)
                                                                                              aaaCCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCg                                                                                                                                                                                                >  1:528720/1‑200 (MQ=255)
                                                                                                aCCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAAc                                                                                                                                           <  1:676341/251‑1 (MQ=255)
                                                                                                          aTTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTc                                                                                                                                                                                                            <  1:635526/176‑1 (MQ=255)
                                                                                                          aTTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGt                                                                                                                                 >  1:676241/1‑251 (MQ=255)
                                                                                                               aaaaaaTGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGAt                                                                                                                            <  1:839873/251‑1 (MQ=255)
                                                                                                                                 aaTTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAatat                                                                                                           >  1:380115/1‑250 (MQ=255)
                                                                                                                                           aGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTg                                                                                                                                                         <  1:580510/194‑1 (MQ=255)
                                                                                                                                           aGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTACCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGaa                                                                                                 >  1:186292/1‑250 (MQ=255)
                                                                                                                                             gTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAAc                                                                                                <  1:302702/249‑1 (MQ=255)
                                                                                                                                                                 aaaaTTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTgg                                                                                                                  >  1:10531/1‑211 (MQ=255)
                                                                                                                                                                   aaTTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCAc                                                                                  <  1:212062/241‑1 (MQ=255)
                                                                                                                                                                   aaTTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAAt                                                                        <  1:143122/251‑1 (MQ=255)
                                                                                                                                                                    aTTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAAtt                                                                       <  1:759070/251‑1 (MQ=255)
                                                                                                                                                                                       ttATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGaa                                                    >  1:854274/1‑251 (MQ=255)
                                                                                                                                                                                                    cgATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGAAGGCACCTTAAcc                                        <  1:364194/250‑1 (MQ=255)
                                                                                                                                                                                                      aTCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTa                                                                                                                                                                                                      <  1:381067/90‑1 (MQ=255)
                                                                                                                                                                                                           gTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATg                                                                                                                                                                 >  1:151971/1‑122 (MQ=255)
                                                                                                                                                                                                                        tAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGAAGGCACCTTAACCTCTAAACGCAAACTCTTaaaa                   <  1:481874/251‑1 (MQ=255)
                                                                                                                                                                                                                        tAAAATTCGCCAGCCATCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGAAGGCACCTTAACCTCTAAACGCAAACTCTTaaaa                   <  1:152786/251‑1 (MQ=255)
                                                                                                                                                                                                                                   agccaTCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGc                                                                                                                                                                                    <  1:160277/79‑1 (MQ=255)
                                                                                                                                                                                                                                   agccaTCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGAAGGCACCTTAACCTCTAAACGCAAACTCTTAAAATTGGTGAatga        >  1:521751/1‑251 (MQ=255)
                                                                                                                                                                                                                                    gccaTCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGt                                                                                                                                                                <  1:151783/98‑1 (MQ=255)
                                                                                                                                                                                                                                    gccaTCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTTTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCTCACCAATATGAATTTTCCCGTTTAAATTTAGAAGTCACCTTAAcc                                        >  1:65658/1‑218 (MQ=255)
                                                                                                                                                                                                                                        tCACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTg                                                                                                                          <  1:412586/132‑1 (MQ=255)
                                                                                                                                                                                                                                         cACCAAACAGGCGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGAAGGCACCTTAACCTCTAAACGCAAACTCTTAAAATTGGTGAATGATGAAAt  >  1:218383/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
TGGATGAACTTTCGCCGGAAGAAATGCGCGAATATCGCGGCACCTTAACCGAACCGGGTAAAAACAGCCCATATCGTGATCGCAGCGTGGAAGAAAACCTGGCTTTATTTGAAAAAATGAAAAACGGCGAATTTGCCGAAGGTAAAGCGAGCTTGCGGGCAAAAATTGATATGGCGTCGCCGTTTATGGTGATGCGCGATCCGGTGCTTTATCGCATAAAATTCGCCAGCCATCACCAAACAGGTGACAAATGGTGCATTTATCCGATGTACGATTTCACTCATTGTATTTCCGATGCTATTGAGCGTATCACGCACTCCTTATGTACCCTTGAATTCCAAGATAACCGTCGTTTGTATGATTGGGTGTTGGAAAATATCAGCATTGAACGCCCGCTGCCGCACCAATATGAATTTTCCCGTTTAAATTTAGAAGGCACCTTAACCTCTAAACGCAAACTCTTAAAATTGGTGAATGATGAAAT  >  1/1430756‑1431239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑