Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,424,400	A→G	N78D (AAT→GAT)	unknown →	Esterase/lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,424,400	0	A	G	100.0%	179.8 / NA	68	N78D (AAT→GAT)	unknown	Esterase/lipase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/26);  total (42/26)

GATTTGACCGCACTTTCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCAATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAAGCAGCGGCAAAAGCGACCATTGACGGCA  >  1/1424166‑1424631                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        gATTTGACCGCACTTTCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATgg                                                                                                                                                                                                                         >  1:599991/1‑250 (MQ=255) gATTTGACCGCACTTTCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTCCGCCCTGTTAGGGGCGAGCGGCGATAAACCGGGTTAtt                                                                                                                                                                                                                          >  1:51792/1‑249 (MQ=255)           cACTTTCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTAttt                                                                                                                                                                                                                <  1:669730/250‑1 (MQ=255)               ttCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCTCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAac                                                                                                                                                                                                           <  1:250784/251‑1 (MQ=255)               ttCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGGGGCGGTAATCCGGGTTATGcccctttttttaaaa                                                                                                                                                                                                           >  1:495833/1‑239 (MQ=255)               ttCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGcc                                                                                                                                                                                                                        <  1:330942/238‑1 (MQ=255)               ttCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTCCGCCTTGTTAGGGGCGAGCGGCGGTAATCCgggt                                                                                                                                                                                                                              >  1:496267/1‑230 (MQ=255)               ttCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGGTTATGCCCATTATTTaaaa                                                                                                                                                                                                            >  1:480438/1‑250 (MQ=255)                tCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTaaaaaa                                                                                                                                                                                                          >  1:105569/1‑249 (MQ=255)                tCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCCTGTTAGGGGCGGGCGGCGATAAACCGGGTTATGCCCCTTATTTtaaaaa                                                                                                                                                                                                          >  1:475843/1‑249 (MQ=255)                      ggCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGGGGTGTCCGCCCTTTTTGGGGCGGGGGGCGATGAACCGGATTATGGCCCTTATTTTAAAAACGCggg                                                                                                                                                                                                    >  1:273975/1‑251 (MQ=255)                            gtggtgGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGcc                                                                                                                                                                                                                        <  1:643240/225‑1 (MQ=255)                            gtggtgGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAAACCGGATTATGCCCCTTATTTAAAAAACGCGGGCGCgg                                                                                                                                                                                               >  1:89445/1‑250 (MQ=255)                                  gCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAg                                                                                                                                                                                         <  1:17817/250‑1 (MQ=255)                                      gtgtGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGGTAATCCGGATTATGCCCCTTATTTAAAACACGCGGGCGCGGCAGAAGGGAgg                                                                                                                                                                                    >  1:86724/1‑250 (MQ=255)                                      gtgtGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGGGCCGGCGGGGCGTTGTCCGCCTTTTTTGGGGGGGGGGGGGGTAATTCGGATTATTCCCCTTATTTTAAACACGCGGGCGGGGCAGAAGGGAgg                                                                                                                                                                                    >  1:745694/1‑250 (MQ=255)                                                             cTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGGTAATCCGGGTTATGCCCCTTATTTAAAACACGCGGGCGCGGCAg                                                                                                                                                                                            >  1:324645/1‑220 (MQ=255)                                                             cTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTCCGCCCTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAAAACGAGGGCGCGGCAGAAGCGAGGGATGCAATTTTTGCGGTTTCTg                                                                                                                                                              >  1:500675/1‑250 (MQ=255)                                                                       aCGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGcc                                                                                                                                                            <  1:503591/242‑1 (MQ=255)                                                                             aaTATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTAcc                                                                                                                                             <  1:477830/251‑1 (MQ=255)                                                                              atatACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGGTTATGCCCCTTATTTAAAACACGCGGGCGCGGCCGAAGCGAGTGATGCCATTTTTGCGGTTTTTGcccttttttcggtttcc                                                                                                                                             >  1:357828/1‑235 (MQ=255)                                                                              atatACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTCCGCCTTGTTTGGGGCGAGCGGCGATAATCCGGGTTATGCCCATTATTTTAAACACGCCGGCGCGGCAGAAGCGAGTGATGCCATTTTTGCGGGTTCTGCCCATTTTTCGAtttcc                                                                                                                                             >  1:657216/1‑247 (MQ=255)                                                                                       cAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAg                                                                                                                                    <  1:711058/250‑1 (MQ=255)                                                                                           gcgcCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTTCGCCTTTTTAGGGGCGAGCGGCGATAATACGGGTTTTGGCCATTATTTAAAACACGCGGGCGGGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTTTGCCTATTGTCCGATTACCAAATTGGGGCACg                                                                                                                                >  1:612407/1‑250 (MQ=255)                                                                                                    gcgATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAgcg                                                                                                                       <  1:66143/250‑1 (MQ=255)                                                                                                               tttGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATggg                                                                                                           <  1:698971/251‑1 (MQ=255)                                                                                                                  gAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGGTGTCCGCCTTGTTAGGGGGGAGCGGCGATAATCCGGGTTATGc                                                                                                                                                                                                                         >  1:232881/1‑138 (MQ=255)                                                                                                                       cggcggTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGa                                                                                                                <  1:725604/238‑1 (MQ=255)                                                                                                                       cggcggTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAAc                                                                                                   <  1:706742/251‑1 (MQ=255)                                                                                                                              gCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGa                                                                                                                                                                                  >  1:19534/1‑165 (MQ=255)                                                                                                                                 ttATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACg                                                                                         <  1:57868/251‑1 (MQ=255)                                                                                                                                       gCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATCACCCGGATTATGCCCATTATTTAAAACACGCGGGCCCCGCCGAAGCGAGTGATGCAATTTTTGCGGTTTCTTCCTATTTTCCGATTACCAATTTGGAGCACGCCGGTAGCGCTTATGAATGGGAGGTTAACGGTTTAAACGAatatt                                                                                   >  1:871530/1‑250 (MQ=255)                                                                                                                                        cATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGTGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAAATCGGAATATGTCCATTATTTAAAACACGCGGGCGCGGCAGaaacg                                                                                                                                                                                       >  1:87724/1‑147 (MQ=255)                                                                                                                                              aaTGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATg                                                                                                                 >  1:772190/1‑214 (MQ=255)                                                                                                                                              aaTGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTAt                                                                            <  1:777093/251‑1 (MQ=255)                                                                                                                                                   tgaGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGata                                                                       <  1:328760/251‑1 (MQ=255)                                                                                                                                                     aGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGGTTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATg                                                                     >  1:841385/1‑251 (MQ=255)                                                                                                                                                     aGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATg                                                                     >  1:79701/1‑251 (MQ=255)                                                                                                                                                      ggTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCgg                                                                                                                                                                    <  1:731785/155‑1 (MQ=255)                                                                                                                                                          aTGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCt                                                                                                                     <  1:498261/198‑1 (MQ=255)                                                                                                                                                          aTGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATg                                                                     >  1:79521/1‑246 (MQ=255)                                                                                                                                                                       ccAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGAc                                                   <  1:348345/251‑1 (MQ=255)                                                                                                                                                                                 tattTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACg                                         <  1:728249/251‑1 (MQ=255)                                                                                                                                                                                   tttCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGAt                                       <  1:60582/251‑1 (MQ=255)                                                                                                                                                                                     tCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCg                                     >  1:564788/1‑251 (MQ=255)                                                                                                                                                                                             cACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTAc                                                                                                                                              <  1:596596/138‑1 (MQ=255)                                                                                                                                                                                             cACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAg                                                                                                         >  1:263774/1‑175 (MQ=255)                                                                                                                                                                                               ccAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAagca                           >  1:341830/1‑251 (MQ=255)                                                                                                                                                                                                cAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAagcag                          >  1:764205/1‑251 (MQ=255)                                                                                                                                                                                                  gTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCCTTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGGGTCGTTTAAATGCGGAc                                                   >  1:681323/1‑224 (MQ=255)                                                                                                                                                                                                  gTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGat                                                                        <  1:580317/203‑1 (MQ=255)                                                                                                                                                                                                  gTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAagc                            <  1:664596/247‑1 (MQ=255)                                                                                                                                                                                                  gTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGACTATGCCCCTTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAAATTTTTCGGTTTTTGCCTATTGTCCGATTAACAATTTGGAGCCCGCCCATAGCGCTTATGAATGGGAGGTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAAGCAGCg                        >  1:517054/1‑251 (MQ=255)                                                                                                                                                                                                        gcggGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAAttt                                                                                                                                                                          >  1:558481/1‑99 (MQ=255)                                                                                                                                                                                                           gggCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAAGCAGCGGCAAAAGCg               >  1:312199/1‑251 (MQ=255)                                                                                                                                                                                                            ggCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAg                                                 >  1:418232/1‑216 (MQ=255)                                                                                                                                                                                                            ggCGTTGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATaa                                           >  1:531223/1‑222 (MQ=255)                                                                                                                                                                                                                ttGTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAatat                                                                                    <  1:110134/177‑1 (MQ=255)                                                                                                                                                                                                                  gTCCGCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAg                                                                                                                                                                                         <  1:470842/74‑1 (MQ=255)                                                                                                                                                                                                                      gCCTTGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCCGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAAc                                          >  1:24952/1‑213 (MQ=255)                                                                                                                                                                                                                         ttGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAAGCAGCGGCAAAAGCGACCATTGACGGCa  >  1:538221/1‑250 (MQ=255)                                                                                                                                                                                                                          tGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGcc                                                                                                                                                            >  1:304639/1‑95 (MQ=255)                                                                                                                                                                                                                          tGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAAt                                                                                                                                          <  1:494948/113‑1 (MQ=255)                                                                                                                                                                                                                          tGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATggg                                                                                                           >  1:604056/1‑144 (MQ=255)                                                                                                                                                                                                                          tGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATaa                                           >  1:789179/1‑208 (MQ=255)                                                                                                                                                                                                                          tGTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACg                                         >  1:168493/1‑210 (MQ=255)                                                                                                                                                                                                                           gTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATaa                                           >  1:846355/1‑207 (MQ=255)                                                                                                                                                                                                                           gTTAGGGGCGAGCGGCGATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATaa                                           >  1:196644/1‑207 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        GATTTGACCGCACTTTCTAAAGGCTATGTGGTGGCGAGTGTGGGCGCACGCGGGCGGACGCTGGAGAAAGACGGAAAATATACCGGCAAGGCGCCGGCGGCGATTATCGATTTGAAATCGGCGGTGCGTTATTTGCATTTTAATGATGAGGTGATGCCGGGCGATGCCAATAAAATTATTTCTAATGGCACCAGTGCCGGCGGGGCGTTGTCCGCCTTGTTAGGGGCGAGCGGCAATAATCCGGATTATGCCCATTATTTAAAACACGCGGGCGCGGCAGAAGCGAGTGATGCAATTTTTGCGGTTTCTGCCTATTGTCCGATTACCAATTTGGAGCACGCCGATAGCGCTTATGAATGGGAGTTTAACGGTTTAAACGAATATCGCCGTATGGATATGAGTCGTTTAAATGCGGACAGTTATAACGATCGTTCTCAAGCAGCGGCAAAAGCGACCATTGACGGCA  >  1/1424166‑1424631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑