Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,417,312	T→C	L123L (TTA→TTG)	unknown ←	Cardiolipin synthase, bacterial type ClsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,417,312	0	T	C	100.0%	212.5 / NA	70	L123L (TTA→TTG)	unknown	Cardiolipin synthase, bacterial type ClsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/35);  total (35/35)

TTTCGGTGATTTCAATGCCTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCTAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGAAACACATAAATCACC  >  1/1417068‑1417558                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       tttCGGTGATTTCAATGCCTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAat                                                                                                                                                                                                                                                   <  1:205224/250‑1 (MQ=255)         aTTTCAATGCCTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTc                                                                                                                                                                                                                                          <  1:634557/251‑1 (MQ=255)          tttCAATGCCTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCg                                                                                                                                                                                                                                         >  1:163923/1‑251 (MQ=255)          tttCAATGCCTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCg                                                                                                                                                                                                                                         >  1:765894/1‑251 (MQ=255)              aaTGCCTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATGTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGc                                                                                                                                                                                                                                     <  1:432605/251‑1 (MQ=255)                  ccTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCATTGCCCAg                                                                                                                                                                                                                                 >  1:482967/1‑251 (MQ=255)                                                  ttttAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTa                                                                                                                                                                                                 <  1:556599/251‑1 (MQ=255)                                                  ttttAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTa                                                                                                                                                                                                 <  1:341556/251‑1 (MQ=255)                                                                            aaTCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAg                                                                                                                                                                        <  1:703727/250‑1 (MQ=255)                                                                                        tACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTa                                                                                                                                                           <  1:218704/251‑1 (MQ=255)                                                                                            aaTTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATag                                                                                                                                                                             >  1:139776/1‑229 (MQ=255)                                                                                              tttCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGccc                                                                                                                                                                                                                                   <  1:785191/173‑1 (MQ=255)                                                                                              tttCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGt                                                                                                                                                            >  1:720560/1‑244 (MQ=255)                                                                                                           ttGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCAc                                                                                                                                         <  1:264273/250‑1 (MQ=255)                                                                                                                     gcgATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTa                                                                                                                                                           <  1:844849/222‑1 (MQ=255)                                                                                                                      ggATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCtt                                                                                                                              >  1:763579/2‑250 (MQ=255)                                                                                                                      cgATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCtt                                                                                                                              >  1:836727/1‑250 (MQ=255)                                                                                                                             ggCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAc                                                                                                                       <  1:530354/250‑1 (MQ=255)                                                                                                                             ggCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAc                                                                                                                       >  1:864328/1‑250 (MQ=255)                                                                                                                              gCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAcc                                                                                                                      <  1:869807/250‑1 (MQ=255)                                                                                                                                 tCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAc                                                                                                                       >  1:281792/1‑246 (MQ=255)                                                                                                                                 tCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATg                                                                                                                   >  1:54544/1‑250 (MQ=255)                                                                                                                                 tCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATg                                                                                                                   >  1:156518/1‑250 (MQ=255)                                                                                                                                     tCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAcc                                                                                                                      <  1:6958/243‑1 (MQ=255)                                                                                                                                        ccTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAg                                                                                                                                                                        <  1:879055/190‑1 (MQ=255)                                                                                                                                        ccTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATaaa                                                                                                            >  1:476726/1‑250 (MQ=255)                                                                                                                                        ccTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATaaa                                                                                                            >  1:567659/1‑250 (MQ=255)                                                                                                                                                     gCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTa                                                                                                                                                                                                 >  1:776399/1‑152 (MQ=255)                                                                                                                                                     gCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGt                                                                                               <  1:531422/250‑1 (MQ=255)                                                                                                                                                      ccTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGt                                                                                               <  1:641972/249‑1 (MQ=255)                                                                                                                                                               gACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAcc                                                                                                                      <  1:423128/217‑1 (MQ=255)                                                                                                                                                               gACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAAc                                                                                      <  1:640793/249‑1 (MQ=255)                                                                                                                                                               gACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAg                                                                                    >  1:458499/1‑251 (MQ=255)                                                                                                                                                                aCCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAAc                                                                                      <  1:427161/248‑1 (MQ=255)                                                                                                                                                                aCCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAg                                                                                    >  1:544756/1‑250 (MQ=255)                                                                                                                                                                 ccAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGa                                                                                                                                                                                                                >  1:272329/1‑125 (MQ=255)                                                                                                                                                                 ccAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCTTGCCATAAACTTCGGTTTCAGTTGATTAAAc                                                                                      >  1:512773/1‑247 (MQ=255)                                                                                                                                                                   aaaTATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCAc                                                                                                                                                      >  1:498120/1‑181 (MQ=255)                                                                                                                                                                     atatAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAcc                                                                                                                      >  1:45324/1‑211 (MQ=255)                                                                                                                                                                     atatAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAAc                                                                                      <  1:212215/243‑1 (MQ=255)                                                                                                                                                                     atatAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAg                                                                                    >  1:828413/1‑245 (MQ=255)                                                                                                                                                                     atatAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCgg                                                                               >  1:350312/1‑250 (MQ=255)                                                                                                                                                                       ataGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGcc                                                                             >  1:498353/1‑250 (MQ=255)                                                                                                                                                                          gAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCgg                                                                          >  1:649913/1‑250 (MQ=255)                                                                                                                                                                           aaCACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGgcg                                                                        <  1:203159/251‑1 (MQ=255)                                                                                                                                                                            acacCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGccc                                                                                                                                                                                                                                   <  1:890717/95‑1 (MQ=255)                                                                                                                                                                                 atatTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGAc                                                                                                                                   <  1:392314/186‑1 (MQ=255)                                                                                                                                                                                         ggATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAAc                                                                                      <  1:360713/223‑1 (MQ=255)                                                                                                                                                                                                      ccTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAAc                                                                                                           >  1:455404/1‑189 (MQ=255)                                                                                                                                                                                                      ccTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAAt                                             >  1:165337/1‑251 (MQ=255)                                                                                                                                                                                                              gTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTgg                                                                                                                                                                   >  1:266939/1‑125 (MQ=255)                                                                                                                                                                                                                   cGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCcaca                                >  1:602281/1‑251 (MQ=255)                                                                                                                                                                                                                   cGATAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCcaca                                >  1:309696/1‑251 (MQ=255)                                                                                                                                                                                                                      tAATGCTGCGCATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATg                             <  1:670332/251‑1 (MQ=255)                                                                                                                                                                                                                             gcgcATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGa                      >  1:677885/1‑251 (MQ=255)                                                                                                                                                                                                                              cgcATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGa                      <  1:737593/250‑1 (MQ=255)                                                                                                                                                                                                                              cgcATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGAt                     <  1:678025/251‑1 (MQ=255)                                                                                                                                                                                                                              cgcATGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACACCACAATGCCTACGAt                     <  1:646188/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAAc                                                                                                                       <  1:336435/149‑1 (MQ=255)                                                                                                                                                                                                                                  tGATACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAg                                                                                    <  1:811173/184‑1 (MQ=255)                                                                                                                                                                                                                                    aTACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAgattgatt                                                        >  1:825343/1‑210 (MQ=255)                                                                                                                                                                                                                                    aTACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGaaa               >  1:625485/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGaaa               >  1:107463/1‑251 (MQ=255)                                                                                                                                                                                                                                     tACTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGAAac              <  1:411803/251‑1 (MQ=255)                                                                                                                                                                                                                                      aCTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAAtt                                                                                                                                                <  1:879585/120‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGcc                                                                                                                                                                                                                  <  1:737769/53‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGAAaca             >  1:550173/1‑250 (MQ=255)                                                                                                                                                                                                                                        tttCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGAAAcaca           >  1:736926/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttCCGGCGTATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGAAACACAt          <  1:427346/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tATCCAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGAAACACATAAATCAcc  <  1:433791/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       TTTCGGTGATTTCAATGCCTTGTTCCTGCATGAGACGGCAATCTTGGCTTTTTAAGAACGGACGGCTGCCCACGGAATCCAGCAAAATACGAATTTCCACACCGCGTTGACGGGCGGCGATAAGGGCTTCCATCACCTCATCAACCAAGCCTTTCGCAGACCAAATATAGAACACCATATTGATGGATTTTTCAGCGCCTTTGATGTCCTCGATAATGCTGCGCATGATACTTTCCGGCGTATCTAAAATATGCAGTTCGTTGCCCAGCACGCAAGGAATGCCGAGGCGTTGTTTGGCTAAATCGAAAATCGGACGATAGAGTAGATTGGTTTGGGTATTCACCAAATTATCGCACTGCGACAGCTTCTCAAACCATGCCATAAACTTCGGTTTCAGTTGATTAAACAGTGCGGCCCGGCGCTTCCCGAGATTGATTTCACCGAAAATCAAATACGCCACAATGCCTACGATGGGAAACACATAAATCACC  >  1/1417068‑1417558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑