Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,395,937	T→C	S83S (TCA→TCG)	unknown ←	Ribulosamine/erythrulosamine 3‑kinase potentially involved in protein deglycation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,395,937	0	T	C	100.0%	176.9 / NA	62	S83S (TCA→TCG)	unknown	Ribulosamine/erythrulosamine 3‑kinase potentially involved in protein deglycation
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/36);  total (26/36)

TTCCGCGCAAAGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGTGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATACGCCCCGAATTGATCAGCTAAAACTTGAGAAAT  >  1/1395700‑1396173                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             ttCCGCGCAAAGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 <  1:317488/251‑1 (MQ=255) ttCCGCGCAAAGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 <  1:607034/251‑1 (MQ=255) ttCCGCGCAAAGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACAGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 <  1:310318/251‑1 (MQ=255)        cAAAGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATacac                                                                                                                                                                                                                          <  1:356096/251‑1 (MQ=255)        cAAAGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCCCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATacac                                                                                                                                                                                                                          <  1:521944/251‑1 (MQ=255)         aaaGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACt                                                                                                                                                                                                                         <  1:409348/251‑1 (MQ=255)             cTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTc                                                                                                                                                                                                                       <  1:1815/249‑1 (MQ=255)             cTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAg                                                                                                                                                                                                                     >  1:443222/1‑251 (MQ=255)              tGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAgg                                                                                                                                                                                                                    <  1:123816/251‑1 (MQ=255)              tGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAgg                                                                                                                                                                                                                    <  1:227996/251‑1 (MQ=255)               gTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACtt                                                                                                                                                                                                                        <  1:340454/246‑1 (MQ=255)                               ccGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 >  1:407522/1‑221 (MQ=255)                                  cTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGtt                                                                                                                                                                                                 <  1:164122/250‑1 (MQ=255)                                      tCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGcc                                                                                                                                                                                            <  1:608137/251‑1 (MQ=255)                                           gAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTaaaa                                                                                                                                                                                       >  1:292303/1‑251 (MQ=255)                                                aCTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGAt                                                                                                                                                                                   >  1:321032/1‑250 (MQ=255)                                                      cccAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCt                                                                                                                                                                                           >  1:842696/1‑236 (MQ=255)                                                          gTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 >  1:838277/1‑194 (MQ=255)                                                          gTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATacac                                                                                                                                                                                                                          <  1:650564/201‑1 (MQ=255)                                                          gTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACgg                                                                                                                                                                                                         <  1:753293/218‑1 (MQ=255)                                                          gTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCg                                                                                                                                                                        >  1:477713/1‑251 (MQ=255)                                                                             ggTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATac                                                                                                                                                                                                                            >  1:101360/1‑180 (MQ=255)                                                                              gTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGa                                                                                                                                                     <  1:121513/250‑1 (MQ=255)                                                                               ttGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAAc                                                                                                                                                   <  1:635506/251‑1 (MQ=255)                                                                                  atatTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATgg                                                                                                                                                      <  1:146989/245‑1 (MQ=255)                                                                                        cGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGAc                                                                                                                                           <  1:758029/250‑1 (MQ=255)                                                                                          gACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAAc                                                                                                                                                   >  1:297923/1‑240 (MQ=255)                                                                                                   aagTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGt                                                                                                                               <  1:847486/251‑1 (MQ=255)                                                                                                              gACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTttc                                                                                                                                      >  1:216242/1‑233 (MQ=255)                                                                                                              gACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGa                                                                                                                     >  1:545788/1‑250 (MQ=255)                                                                                                                          ttttCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGcc                                                                                                        <  1:886182/251‑1 (MQ=255)                                                                                                                           tttCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACAcc                                                                                                                                                                                                                                >  1:243540/1‑130 (MQ=255)                                                                                                                           tttCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGATGTTGGTTTTGCCTAAAAg                                                                                                                                                                                      <  1:584185/172‑1 (MQ=255)                                                                                                                                gAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 <  1:220103/124‑1 (MQ=255)                                                                                                                                  gCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 <  1:142090/122‑1 (MQ=255)                                                                                                                                              gTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGAtt                                                                                    >  1:694682/1‑251 (MQ=255)                                                                                                                                              gTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGAt                                                                                     <  1:824843/250‑1 (MQ=255)                                                                                                                                               tGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGAtt                                                                                    <  1:150817/250‑1 (MQ=255)                                                                                                                                                   aaCGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGAc                                                                                                                                           >  1:128767/1‑191 (MQ=255)                                                                                                                                                   aaCGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATg                                                                                >  1:28793/1‑250 (MQ=255)                                                                                                                                                                     cGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAAtt                                                             <  1:275590/251‑1 (MQ=255)                                                                                                                                                                     cGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCAAACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAAtt                                                             <  1:157740/251‑1 (MQ=255)                                                                                                                                                                              ccATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCacac                                                                                                                                                                                                                                 >  1:587740/1‑78 (MQ=255)                                                                                                                                                                                atatTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATg                                                                                              >  1:332841/1‑207 (MQ=255)                                                                                                                                                                                           cgTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGa                                                                                                                                                     <  1:738517/141‑1 (MQ=255)                                                                                                                                                                                            gTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATac                                                                                                                                                                                                                            >  1:770431/1‑69 (MQ=255)                                                                                                                                                                                            gTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGcc                                                                       >  1:184050/1‑218 (MQ=255)                                                                                                                                                                                            gTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGaata                                      >  1:548465/1‑251 (MQ=255)                                                                                                                                                                                                tCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATAc                                  <  1:153910/251‑1 (MQ=255)                                                                                                                                                                                                tCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATAc                                  <  1:138734/251‑1 (MQ=255)                                                                                                                                                                                                tCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATAc                                  <  1:626521/251‑1 (MQ=255)                                                                                                                                                                                                         tatTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGa                                                                                                                                                     <  1:389155/127‑1 (MQ=255)                                                                                                                                                                                                         tatTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGAt                                            <  1:548428/232‑1 (MQ=255)                                                                                                                                                                                                         tatTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATAc                                  >  1:408976/1‑242 (MQ=255)                                                                                                                                                                                                          atTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTc                                                                                                                                                                                                                       >  1:706351/1‑60 (MQ=255)                                                                                                                                                                                                          atTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATAc                                  >  1:568088/1‑241 (MQ=255)                                                                                                                                                                                                          atTTAAGCCTTCCAATAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATACGCCCCGAAtt                        >  1:220000/1‑251 (MQ=255)                                                                                                                                                                                                                       aaTAACAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATACGCCCCGAATTGATCAGCTAAAAc           <  1:162283/251‑1 (MQ=255)                                                                                                                                                                                                                           aCAAAAAACTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGcc                                                                       <  1:361788/187‑1 (MQ=255)                                                                                                                                                                                                                                 aaCTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATACGCCCCGAATTGATCAGCTAAAACTTGAGAAAt  >  1:571976/1‑250 (MQ=255)                                                                                                                                                                                                                                 aaCTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATACGCCCCGAATTGATCAGCTAAAACTTGAGAAAt  >  1:35147/1‑250 (MQ=255)                                                                                                                                                                                                                                   cTATGATTTTGCGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGtt                                                                                                                                                                                                 <  1:113240/57‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             TTCCGCGCAAAGCTGTAATTGCCAGCCAATCCGCTGTTCGCTGAAAAACTTCGCCCAGTGGTTGCTCCATTCGTTCGGTTGATATTGCGGACCAAGCCAAGTATCAAAAGACAAACCGTAGTTTTCTGAGCCGTGATATTGGTGCAAACGGGCGAGTTGCTCACCGAATTCCGCCATATTTTGCGGCGTATTCGGTTGCATATTTAAGCCTTCCAATAACAAAAAACTATGATTTTGTGAGCATCCCACACCATACACTTCAGGCACGCGTACGGTGTTGGTTTTGCCTAAAAGGATCAATTGATCCGCTTCGGCGCGGAACATGGAACGGTAGGACTTTTCATTCAGTTTTACGAAGACGGGTTGAATGCCGTCATTAATGAGCCAAGATTCATGCATTTCGCCCGTATGAATTTTGGTTTTATTTTTGATGGAATAATACGCCCCGAATTGATCAGCTAAAACTTGAGAAAT  >  1/1395700‑1396173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑