Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,378,690	A→G	D160D (GAT→GAC)	unknown ←	Fructose repressor FruR, LacI family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,378,690	0	A	G	100.0%	197.2 / NA	67	D160D (GAT→GAC)	unknown	Fructose repressor FruR, LacI family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/37);  total (30/37)

CCGTTGGTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCATCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTGATAGCCTTTTTCCCGTAAACGATTTTCCAGATTA  >  1/1378455‑1378927                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              ccGTTGGTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGc                                                                                                                                                                                                                                 <  1:542670/250‑1 (MQ=255)   gTTGGTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCAtt                                                                                                                                                                                                                              >  1:290788/1‑251 (MQ=255)     tGGTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGt                                                                                                                                                                                                                                       >  1:363256/1‑240 (MQ=255)     tGGTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTca                                                                                                                                                                                                                            >  1:32511/1‑251 (MQ=255)      ggTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTtcgtcg                                                                                                                                                                                                                                            <  1:32418/234‑1 (MQ=255)       gTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACAGATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTcac                                                                                                                                                                                                                           <  1:246569/250‑1 (MQ=255)        tAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTcacc                                                                                                                                                                                                                          <  1:767183/250‑1 (MQ=255)         aaCGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTcaccac                                                                                                                                                                                                                        <  1:32546/251‑1 (MQ=255)            ggATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACttt                                                                                                                                                                                                                     >  1:591325/1‑251 (MQ=255)                         aCCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATa                                                                                                                                                                                                        <  1:648895/251‑1 (MQ=255)                                      aCATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATa                                                                                                                                                                                                        >  1:274755/1‑238 (MQ=255)                                      aCATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCaaaa                                                                                                                                                                                            >  1:703902/1‑250 (MQ=255)                                      aCATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAAc                                                                                                                                                                                           <  1:4551/251‑1 (MQ=255)                                                         ttACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTgg                                                                                                                                                                         >  1:755059/1‑250 (MQ=255)                                                           aCGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATAc                                                                                                                                                                                                       <  1:843772/218‑1 (MQ=255)                                                                     tCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTaaaa                                                                                                                                                            >  1:755531/1‑251 (MQ=255)                                                                        ccGTAAGGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATc                                                                                                                                                         <  1:267054/251‑1 (MQ=255)                                                                                           aaGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGc                                                                                                                                       <  1:558655/250‑1 (MQ=255)                                                                                              ccTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGc                                                                                                                                                                                                                                 <  1:527099/157‑1 (MQ=255)                                                                                              ccTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAAc                                                                                                                                                                                           <  1:364586/195‑1 (MQ=255)                                                                                              ccTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGa                                                                                                                                    <  1:94243/250‑1 (MQ=255)                                                                                               cTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAgg                                                                                                                                  <  1:446204/251‑1 (MQ=255)                                                                                                 tCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGaa                                                                                                                                >  1:2706/1‑251 (MQ=255)                                                                                                     aTACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTcacc                                                                                                                                                                                                                          <  1:759566/157‑1 (MQ=255)                                                                                                     aTACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAAcc                                                                                                                             <  1:369844/250‑1 (MQ=255)                                                                                                       aCCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACcaac                                                                                                                          <  1:174170/251‑1 (MQ=255)                                                                                                        ccTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCgggg                                                                                                                                                                                                                >  1:312730/1‑164 (MQ=255)                                                                                                        ccTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACgg                                                                                                                                                                                                     <  1:754675/175‑1 (MQ=255)                                                                                                        ccTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATc                                                                                                                                                         <  1:45425/219‑1 (MQ=255)                                                                                                        ccTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACcaaca                                                                                                                         >  1:7427/1‑251 (MQ=255)                                                                                                        ccTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACcaaca                                                                                                                         >  1:556089/1‑251 (MQ=255)                                                                                                        ccTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACcaaca                                                                                                                         >  1:747811/1‑251 (MQ=255)                                                                                                            aaTGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGc                                                                                                                     <  1:380438/251‑1 (MQ=255)                                                                                                                   aGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATAc                                                                                                                                                                                                       >  1:424704/1‑162 (MQ=255)                                                                                                                       aaaTCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCaa                                                                                                                >  1:230196/1‑245 (MQ=255)                                                                                                                         aTCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGt                                                                                                        <  1:601519/251‑1 (MQ=255)                                                                                                                           ccTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTg                                                                                                      >  1:271567/1‑251 (MQ=255)                                                                                                                           ccTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTg                                                                                                      >  1:23966/1‑251 (MQ=255)                                                                                                                                   ccgttcccgGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAAt                                                                                              <  1:396638/251‑1 (MQ=255)                                                                                                                                   ccgttcccgGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAAt                                                                                              <  1:496012/251‑1 (MQ=255)                                                                                                                                      ttcccgGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAAt                                                                                              >  1:471498/1‑248 (MQ=255)                                                                                                                                      ttcccgGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGtt                                                                                           >  1:7101/1‑251 (MQ=255)                                                                                                                                      ttcccgGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGt                                                                                            <  1:374952/250‑1 (MQ=255)                                                                                                                                           ggCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGc                                                                                                                                       <  1:32062/202‑1 (MQ=255)                                                                                                                                             cTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGc                                                                                     <  1:468358/250‑1 (MQ=255)                                                                                                                                                      aaaTCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTc                                                                           <  1:252791/251‑1 (MQ=255)                                                                                                                                                        aTCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCAt                                                                         >  1:571356/1‑251 (MQ=255)                                                                                                                                                        aTCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCAt                                                                         >  1:599865/1‑251 (MQ=255)                                                                                                                                                        aTCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCAt                                                                         >  1:344712/1‑251 (MQ=255)                                                                                                                                                             gTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACgg                                                                                                                                                                                                     >  1:237057/1‑122 (MQ=255)                                                                                                                                                             gTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGt                                                                                                        <  1:638124/215‑1 (MQ=255)                                                                                                                                                             gTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTc                                                                     >  1:663308/1‑250 (MQ=255)                                                                                                                                                               aaCGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCAc                                                                  <  1:307518/251‑1 (MQ=255)                                                                                                                                                                  gCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACttt                                                               >  1:498592/1‑251 (MQ=255)                                                                                                                                                                            aCAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGaa                                                     >  1:94009/1‑251 (MQ=255)                                                                                                                                                                                      tttttGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACcaac                                                                                                                          <  1:8217/172‑1 (MQ=255)                                                                                                                                                                                       ttttGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGag                                          <  1:135026/251‑1 (MQ=255)                                                                                                                                                                                             tgaGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCGCCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCAGGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTg                                    <  1:263726/251‑1 (MQ=255)                                                                                                                                                                                             tgaGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTCATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTg                                    <  1:464951/251‑1 (MQ=255)                                                                                                                                                                                             tgaGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTg                                    <  1:328226/251‑1 (MQ=255)                                                                                                                                                                                                 ggATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTGAt                                  <  1:216452/249‑1 (MQ=255)                                                                                                                                                                                                   aTTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGa                                                      <  1:380824/227‑1 (MQ=255)                                                                                                                                                                                                   aTTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATg                                            <  1:279789/237‑1 (MQ=255)                                                                                                                                                                                                                   tCGTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTGATAGCCTTTTTCCCGTAAACGa              >  1:324215/1‑251 (MQ=255)                                                                                                                                                                                                                     gTTAAACGATATGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATc                                                                                                                                                         <  1:685437/110‑1 (MQ=255)                                                                                                                                                                                                                             atatGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACcaac                                                                                                                          >  1:646465/1‑133 (MQ=255)                                                                                                                                                                                                                               atGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGtaataa                                                                                                >  1:334852/1‑157 (MQ=255)                                                                                                                                                                                                                               atGCATCGCCTTCGTCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCAATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTGATAGCCTTTTTCCCGTAAACGATTTTCCAGatta  >  1:729272/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              CCGTTGGTAACGGATTATTTTCTAACCATTGGGCAAAACATTGCGCCGCTTCGTTTTTACGGAACATATCGCCGTAAAGATATTTGGCGGAAGCCTTCCGATACCTTAATGCCGAGCGAAATCCTTCTTCCCGTTCCCGGCTTGTCGGCAAATCCGGTAACGCACCGAAGAACAAAATTCTTTTTTGATGAGGGATTTTCAACAATTCCTTCGTTAAACGATATGCATCGCCTTCATCGTCGGTCAGGGCATTCACCACTTTCGGGGAGCTAATACGGCGATCAAAACCGATAATCGGCACGCTGGCGTTTTGGTAAAAATCTGTGTTCGGTGGCAGTGCTGAGGAAACCAACAATGCATCAACCTGACGTTGTAATAAATGTTTCACGCAATCTTGCTCATTTTCCACTTTGTCGTTAGAACAGGCGATGAGTAGTTGATAGCCTTTTTCCCGTAAACGATTTTCCAGATTA  >  1/1378455‑1378927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑