Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,327,590	G→A	intergenic (‑326/‑142)	unknown ← / → unknown	hypothetical protein/Fic domain protein, MA2133 type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,327,590	0	G	A	100.0%	102.1 / NA	37	intergenic (‑326/‑142)	unknown/unknown	hypothetical protein/Fic domain protein, MA2133 type
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/20);  total (17/20)

AAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCCAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAGGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCGCTTGACCTCATTACAGCAAAATCCTTACAAGATGATTTTATGC  >  1/1327352‑1327822                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         aaaaaaTAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCaaaaaa                                                                                                                                                                                                                              <  1:150938/251‑1 (MQ=255)                      acTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCCAAGAGAACAAGCATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGTAGCCAAAAAATATGTGTTATTAAACGATAtt                                                                                                                                                                                                         >  1:337322/1‑251 (MQ=255)                       cTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTa                                                                                                                                                                                                        >  1:464147/1‑251 (MQ=255)                                aaaTAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATc                                                                                                                                                                                                     >  1:575945/1‑245 (MQ=255)                                              aTTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATg                                                                                                                                                                                   <  1:825494/249‑1 (MQ=255)                                                 aatcaaatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATc                                                                                                                                                                                                     <  1:355135/228‑1 (MQ=255)                                                 aatcaaatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTagaag                                                                                                                                                                                               <  1:179788/234‑1 (MQ=255)                                                      aatcaCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGcc                                                                                                                                                                         <  1:895794/251‑1 (MQ=255)                                                         caCTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATg                                                                                                                                                                                   >  1:198912/1‑238 (MQ=255)                                                           cTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGCACCCGCCCAAgg                                                                                                                                                                    <  1:256814/251‑1 (MQ=255)                                                                   aTTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACag                                                                                                                                                            <  1:159829/251‑1 (MQ=255)                                                                              cTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGAtt                                                                                                                                                 <  1:717229/251‑1 (MQ=255)                                                                                    gaACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACag                                                                                                                                                            >  1:825201/1‑234 (MQ=255)                                                                                         aGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATAtgtg                                                                                                                                                                                                                        <  1:837118/169‑1 (MQ=255)                                                                                         aGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTAtaaataaa                                                                                                                                      >  1:633356/1‑251 (MQ=255)                                                                                          gTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATAtgtg                                                                                                                                                                                                                        >  1:547065/1‑168 (MQ=255)                                                                                                  aaaCTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTagaag                                                                                                                                                                                               >  1:357609/1‑185 (MQ=255)                                                                                                   aaCTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATc                                                                                                                                                                                                     <  1:571062/178‑1 (MQ=255)                                                                                                   aaCTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTaa                                                                                                                                                                                     <  1:506008/194‑1 (MQ=255)                                                                                                                         gACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACttt                                                                                                                                                                                       >  1:135511/1‑170 (MQ=255)                                                                                                                           cAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATgg                                                                                                    <  1:336309/251‑1 (MQ=255)                                                                                                                                 gggTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTaaa                                                                                              >  1:514392/1‑251 (MQ=255)                                                                                                                                      atttatGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACag                                                                                                                                                            <  1:116868/184‑1 (MQ=255)                                                                                                                                      atttatGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGa                                                                                         >  1:664246/1‑251 (MQ=255)                                                                                                                                                      gCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGa                                                                                                                                                                                                             >  1:632557/1‑119 (MQ=255)                                                                                                                                                             cAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAAt                                                                                                                      >  1:608582/1‑199 (MQ=255)                                                                                                                                                                     ccACAGCTAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCTAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTgg                                                           <  1:456291/250‑1 (MQ=255)                                                                                                                                                                            tAAACAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAg                                                   <  1:662515/251‑1 (MQ=255)                                                                                                                                                                                cAAAAGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGAt                                                                                                                                                  <  1:440913/152‑1 (MQ=255)                                                                                                                                                                                  aaaGAAGAGGTGACGGTTGCTAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCg                                             >  1:751681/1‑251 (MQ=255)                                                                                                                                                                                                                 cAACTATCATGAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCGCTTGACCTCATTACAGc                            <  1:786530/237‑1 (MQ=255)                                                                                                                                                                                                                           gAGCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAg                                                   <  1:764468/204‑1 (MQ=255)                                                                                                                                                                                                                             gCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTGAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCGCTTGACCTCATTACAGCAAAATCCTTACAAGATGATTATATg   >  1:172084/1‑250 (MQ=255)                                                                                                                                                                                                                             gCTCTGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGAc                                                                              >  1:596145/1‑175 (MQ=255)                                                                                                                                                                                                                              ctctGTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCGCTTGACCTCATTACAGCAAAATCCTTACAAGATGATTTTATGc  <  1:777006/250‑1 (MQ=255)                                                                                                                                                                                                                                  gTTATGTTTATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTa                                                                                                          >  1:14476/1‑142 (MQ=255)                                                                                                                                                                                                                                         ttATAAAGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAggg                                                                                                                                                    <  1:866865/93‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         AAAAAATAAATTTGTGTAACAACTTTATGCGAAATAAAATCAGAGATTAATCAAATCACTAATAAAATTTTACCGCGCTAAGAGAACAAGTATGAGTAAACTAACAAAGGTCATTAATCAGACAATCCGGGTTATTTATGGGAACGATTGCAAAAACAGCTATGCCACAGCTAAACAAAAGAAGAGGTGACGGTTGCCAAATAACGTACAACTATCATGAGCTCTGTTATGTTTATAAGGCAGCCAAAAAATATGTGTTATTAAACGATATTAATCTAGAAGAGTACTTTAATGAGTACCCGCCCAAGGTTTAACAGAGAAAGGGATTTTATAAATAAATGCATAAAAACATAATTATGTTTTAGTATGATGGAGTAAATATGAATATCTTAGACGAACTAAAGAAAGTATTGGATTCAAAGCGTCCGCTTGACCTCATTACAGCAAAATCCTTACAAGATGATTTTATGC  >  1/1327352‑1327822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑