Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,312,755 T→C I338T (ATA→ACA)  unknown → Type III restriction‑modification system methylation subunit (EC 2.1.1.72)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,312,7550TC100.0% 92.6 / NA 33I338T (ATA→ACA) unknownType III restriction‑modification system methylation subunit (EC 2.1.1.72)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

TGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCCTCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGATAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAATAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTAAATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGG  >  1/1312513‑1312974
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           
tGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCGTCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAaccacac                                                                                                                                                                                                                     <  1:570972/251‑1 (MQ=255)
      gccgTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTa                                                                                                                                                                                                               >  1:798706/1‑251 (MQ=255)
                                              gTCTATAAAGATGGCTATCATCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAAt                                                                                                                                                                                       <  1:602467/235‑1 (MQ=255)
                                                                                                          gCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATAc                                                                                                                                                                            >  1:59568/1‑186 (MQ=255)
                                                                                                           ccTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTgg                                                                                                                                   >  1:189226/1‑226 (MQ=255)
                                                                                                                  gagCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGagaatagaat                                                                                                                                               >  1:51767/1‑207 (MQ=255)
                                                                                                                      aTTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAAt                                                                                               >  1:482695/1‑251 (MQ=255)
                                                                                                                         ttGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAAttt                                                                                                                                                                                                           >  1:17915/1‑140 (MQ=255)
                                                                                                                                aaGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGaca                                                                                     <  1:219921/251‑1 (MQ=255)
                                                                                                                                           tttttATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAgg                                                                          <  1:6596/251‑1 (MQ=255)
                                                                                                                                             tttATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAgggg                                                                        <  1:21840/251‑1 (MQ=255)
                                                                                                                                             tttATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAgggg                                                                        <  1:339957/251‑1 (MQ=255)
                                                                                                                                                      tATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTac                                                                 <  1:733692/249‑2 (MQ=255)
                                                                                                                                                       aTTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAAttt                                                                                                                                                                                                           <  1:86319/110‑1 (MQ=255)
                                                                                                                                                             gatAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAaccacaccac                                                                                                                                                                                                                  >  1:269602/1‑97 (MQ=255)
                                                                                                                                                                   gagaTTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGaa                                                                                                                           <  1:405173/178‑1 (MQ=255)
                                                                                                                                                                     gaTTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGAtatta                                                >  1:70349/1‑251 (MQ=255)
                                                                                                                                                                           cAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAGCCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATtacta                                          <  1:648855/251‑1 (MQ=255)
                                                                                                                                                                                   cagatatTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATtactac                                         >  1:127101/4‑244 (MQ=255)
                                                                                                                                                                                        atTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAgg                                                                          >  1:666779/1‑206 (MQ=255)
                                                                                                                                                                                               aTTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAgggg                                                                        >  1:141773/1‑201 (MQ=255)
                                                                                                                                                                                                  ataCTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGac                                                                                      >  1:890310/1‑184 (MQ=255)
                                                                                                                                                                                                    aCTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGc                                                                                                                                                                    <  1:345042/104‑1 (MQ=255)
                                                                                                                                                                                                      gctAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGaaaaaac                                                                                                                      <  1:35388/147‑2 (MQ=255)
                                                                                                                                                                                                         aGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGc                                                                                                                                                                    <  1:533997/99‑1 (MQ=255)
                                                                                                                                                                                                         aGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAgggg                                                                        >  1:489885/1‑191 (MQ=255)
                                                                                                                                                                                                         aGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAgg                                                                          >  1:379289/1‑189 (MQ=255)
                                                                                                                                                                                                          ggTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTgg           >  1:805012/1‑251 (MQ=255)
                                                                                                                                                                                                                gATAACTTTATTGATATTTTCTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCtt                                                                                                                                                                  <  1:8303/94‑1 (MQ=255)
                                                                                                                                                                                                                 aTAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTAc    <  1:398852/251‑1 (MQ=255)
                                                                                                                                                                                                                 aTAACTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTAc    <  1:691671/251‑1 (MQ=255)
                                                                                                                                                                                                                    aCTTTATTGATATTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAACAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTACATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACgg  >  1:213946/1‑250 (MQ=255)
                                                                                                                                                                                                                              tatTTTTTCTTGGCAAAAGACAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTg                                                                                                                                    >  1:729296/1‑110 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           
TGATCCGCCGTATAATACAGAAAAAGATAGAATTGATGGAAAATTTGTCTATAAAGATGGCTATCCTCATTCTACATGGGCATCTTTTTTATATAATTTATCAAAAGCCTCCAAGAGCATTTTGAATAAAGATGCAATTTTTTTATCAAGTATTGATGATAATGAGATTATCAATCTGAAAAATATTTTAGATTATACTCTAGGTAAAGATAACTTTATTGATATTTTTTCTTGGCAAAAGATAACCACACCACCTAATTTATCTAAACAAACAAAACAATCTTGCGAATACATTTTAGCTTATCAAAGTGAGAATAGAATATCTCTTACTGGTATTAGAAAAAATAGTAAGAGTTCAAATGGTTTAATGAATCAGACAAACAATATAGGGGTTCTTAAATTTCCTTCAGATATTATTACTACAAAATTACCAGATGGGATTTATAAGGCTGGTATGTACGG  >  1/1312513‑1312974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑