Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,306,878	A→G	intergenic (+89/‑67)	unknown → / → unknown	tRNA‑Met‑CAT/Bacterial ribosome SSU maturation protein RimP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,306,878	0	A	G	100.0%	77.9 / NA	27	intergenic (+89/‑67)	unknown/unknown	tRNA‑Met‑CAT/Bacterial ribosome SSU maturation protein RimP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

CTATAATACGCCACATATTTCGGACGCGGGGTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTATTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGCCGTCAGGT  >  1/1306689‑1307120                                                                                                                                                                                              |                                                                                                                                                                                                                                                   cTATAATACGCCACATATTTCGGACGCGGGGTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAAc                                                                                                                                                                                                                                 >  1:168254/1‑209 (MQ=255)          gCCACATATTTCGGACGCGGGGTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTg                                                                                                                                                                                              <  1:90409/235‑1 (MQ=255)                             gggTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAAtt                                                                                                                                                           >  1:316888/1‑251 (MQ=255)                             gggTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAAtt                                                                                                                                                           >  1:495055/1‑251 (MQ=255)                                                            aTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGAt                                                                                                                            <  1:275010/251‑1 (MQ=255)                                                               aCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTc                                                                                                                         <  1:311838/251‑1 (MQ=255)                                                                       gtcgtcGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCa                                                                                                                 >  1:332134/1‑251 (MQ=255)                                                                                                             gTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGt                                                                                                                                   >  1:589480/1‑195 (MQ=255)                                                                                                              ttCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTAtt                                                                           <  1:745854/250‑1 (MQ=255)                                                                                                                  gTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACa                                                                                                                                            >  1:22042/1‑181 (MQ=255)                                                                                                                   tAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAAc                                                                       <  1:190795/249‑1 (MQ=255)                                                                                                                          aaCCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCgtt                                                               <  1:470352/250‑1 (MQ=255)                                                                                                                                                               ttgttTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGAt                         <  1:4136/251‑1 (MQ=255)                                                                                                                                                                 gttTAAATATCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAAc                                                                       <  1:7347/203‑1 (MQ=255)                                                                                                                                                                       atatCAAATAATTAATTATAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAg                                                                                                                                          <  1:625556/130‑1 (MQ=255)                                                                                                                                                                                   taattaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGCCGTCa     >  1:627215/1‑251 (MQ=255)                                                                                                                                                                                    aattaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAAGTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAAc                                                                       <  1:68418/184‑1 (MQ=255)                                                                                                                                                                                    aattaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCgtttg                                                             <  1:370623/194‑1 (MQ=255)                                                                                                                                                                                    aattaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTg                                                      >  1:332277/1‑201 (MQ=255)                                                                                                                                                                                    aattaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGc          >  1:89149/1‑245 (MQ=255)                                                                                                                                                                                    aattaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGCCGTCAg    >  1:631581/1‑251 (MQ=255)                                                                                                                                                                                    aattaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGCCGTCAg    >  1:292378/1‑251 (MQ=255)                                                                                                                                                                                     attaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAg                                                                                                                                          >  1:795605/1‑116 (MQ=255)                                                                                                                                                                                     attaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACgg                                 <  1:572512/221‑1 (MQ=255)                                                                                                                                                                                     attaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGCCGTCAgg   >  1:691128/1‑251 (MQ=255)                                                                                                                                                                                      ttaTAACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGCCGTCAGGt  >  1:711654/1‑251 (MQ=255)                                                                                                                                                                                        ataACTGTTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAAc                                                                       >  1:795520/1‑180 (MQ=255)                                                                                                                                                                                              |                                                                                                                                                                                                                                                   CTATAATACGCCACATATTTCGGACGCGGGGTGGAGCAGCTTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACAAGTTCAGTAAAAAGAACCCCAATTTTGGGGTTTTTTTATACGATAAATTTGTTGTTTAAATATCAAATAATTAATTATAACTATTTGATAATTATTGAAAACTGGGCTGTAAGCCCTTTTTTTACGTCTATCGTTTGAGGGAGGTTGTTTTGGCAACTTTGGAACAAAAATTACAGGATTTATTACAAGGCTCGGTGGAAGATTTCGGTTGTCAGCTTTGGGGCATTGAATGCCAGCGTGTCGGTCGTTATTTAACCGTGCGTTTGTTTATTGATAAAGAAGGCGGTGTGACGGTGGAAGATTGCGCCGATGTGAGCCGTCAGGT  >  1/1306689‑1307120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑