Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,296,250	G→C	L68L (CTC→CTG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,296,250	0	G	C	100.0%	151.8 / NA	51	L68L (CTC→CTG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/26);  total (25/26)

TATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTAATTTTTTAAGCAAAAAAGCTTTGTTTCCGCCTTCGAGCACAAAAGCTTCGCCTCCCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATCCCCCGATTGCGTCATATGAATTGCAAACTATCGCTTGGGCGTGCTTCGC  >  1/1296008‑1296487                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              tatTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACaaa                                                                                                                                                                                                                                       >  1:372098/1‑251 (MQ=255)       gCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCtt                                                                                                                                                                                                                                  >  1:102854/1‑250 (MQ=255)            aTCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCt                                                                                                                                                                                                                             >  1:755772/1‑250 (MQ=255)            aTCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCt                                                                                                                                                                                                                             >  1:598807/1‑250 (MQ=255)                               ggCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAAt                                                                                                                                                                                                          >  1:765734/1‑250 (MQ=255)                                   tAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAAc                                                                                                                                                                                                     <  1:604737/251‑1 (MQ=255)                                    aGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACg                                                                                                                                                                                                    >  1:853795/1‑251 (MQ=255)                                             aCGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCt                                                                                                                                                                                           <  1:153904/251‑1 (MQ=255)                                                    ggCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTg                                                                                                                                                                                    >  1:104007/1‑251 (MQ=255)                                                                ggCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCgaga                                                                                                                                                                         >  1:473612/1‑250 (MQ=255)                                                                      ggTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAacac                                                                                                                                                                   <  1:23451/250‑1 (MQ=255)                                                                          ctcGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGAt                                                                                                                                                              <  1:862477/251‑1 (MQ=255)                                                                          ctcGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGAt                                                                                                                                                              <  1:813220/251‑1 (MQ=255)                                                                                acaTGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTcc                                                                                                                                                        <  1:617054/251‑1 (MQ=255)                                                                                     ccGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTc                                                                                                                                                   <  1:68208/251‑1 (MQ=255)                                                                                             gTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATc                                                                                                                                                                                                         <  1:31273/189‑1 (MQ=255)                                                                                             gTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCt                                                                                                                                                                                      <  1:373634/208‑1 (MQ=255)                                                                                                 aaaTCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCaa                                                                                                                                       <  1:49810/251‑1 (MQ=255)                                                                                                     ctctACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAAc                                                                                                                                                                                                     >  1:232723/1‑185 (MQ=255)                                                                                                     ctctACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTc                                                                                                                                                   <  1:372580/235‑1 (MQ=255)                                                                                                       ctACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGc                                                                                                                                         >  1:634013/1‑243 (MQ=255)                                                                                                                ccGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTg                                                                                                                                                                                    >  1:565227/1‑191 (MQ=255)                                                                                                                               ttATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGaca                                                                                                                                                                       >  1:679958/1‑189 (MQ=255)                                                                                                                               ttATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGaa                                                                                                          >  1:656074/1‑250 (MQ=255)                                                                                                                               ttATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGaa                                                                                                          >  1:45851/1‑250 (MQ=255)                                                                                                                                    aTCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAg                                                                                                            <  1:65448/243‑1 (MQ=255)                                                                                                                                    aTCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAAt                                                                                                    >  1:832235/1‑251 (MQ=255)                                                                                                                                          tgctgcGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGAt                                                                                              <  1:350230/251‑1 (MQ=255)                                                                                                                                                  cAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCgg                                                                                      <  1:702551/251‑1 (MQ=255)                                                                                                                                                    aTCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTg                                                                                    <  1:772387/251‑1 (MQ=255)                                                                                                                                                     tctcTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTcc                                                                                                                                                        <  1:23072/182‑1 (MQ=255)                                                                                                                                                      ctctAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTc                                                                                                                                                   >  1:663032/1‑186 (MQ=255)                                                                                                                                                      ctctAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAg                                                                                  >  1:152494/1‑251 (MQ=255)                                                                                                                                                      ctctAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAg                                                                                  >  1:265119/1‑251 (MQ=255)                                                                                                                                                             tctggttcGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCg                                                                             <  1:116601/242‑1 (MQ=255)                                                                                                                                                                ggttcGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAacac                                                                                                                                                                   <  1:33126/156‑1 (MQ=255)                                                                                                                                                                      gACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAgg                                                                                                                                                           >  1:448139/1‑162 (MQ=255)                                                                                                                                                                        cTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACAcc                                                                 <  1:755870/250‑1 (MQ=255)                                                                                                                                                                          cATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGc                                                                                                                                         >  1:837863/1‑176 (MQ=255)                                                                                                                                                                          cATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAg                                                               >  1:788769/1‑250 (MQ=255)                                                                                                                                                                                aaCCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTacac                                                        <  1:535062/251‑1 (MQ=255)                                                                                                                                                                                aaCCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTacac                                                        <  1:488132/251‑1 (MQ=255)                                                                                                                                                                                aaCCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTacac                                                        <  1:822461/251‑1 (MQ=255)                                                                                                                                                                                         aaTCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATcccc                                                >  1:484687/1‑250 (MQ=255)                                                                                                                                                                                         aaTCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATcccc                                                >  1:417761/1‑250 (MQ=255)                                                                                                                                                                                          aTCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATCCCCCg                                              >  1:350783/1‑251 (MQ=255)                                                                                                                                                                                                 cATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGa                                                                                                                                    <  1:513087/158‑1 (MQ=255)                                                                                                                                                                                                                           cAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGc                                                                                                                                         <  1:363/127‑1 (MQ=255)                                                                                                                                                                                                                           cAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGAc                                                                                                                                   >  1:678024/1‑133 (MQ=255)                                                                                                                                                                                                                             aataaGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAAt                                                                                                                               <  1:34289/132‑1 (MQ=255)                                                                                                                                                                                                                                      tGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATCCCCCGATTGCGTCATAccgtaattgccatttaaaaacgcgtgtttttct  <  1:138690‑M1/251‑34 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              TATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTAATTTTTTAAGCAAAAAAGCTTTGTTTCCGCCTTCGAGCACAAAAGCTTCGCCTCCCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATCCCCCGATTGCGTCATATGAATTGCAAACTATCGCTTGGGCGTGCTTCGC  >  1/1296008‑1296487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑