Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,296,230	A→T	F75Y (TTT→TAT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,296,230	0	A	T	100.0%	148.9 / NA	53	F75Y (TTT→TAT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (28/25);  total (28/25)

AAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTAATTTTTTAAGCAAAAAAGCTTTGTTTCCGCCTTCGAGCACAAAAGCTTCGCCTCCCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATCCCCCG  >  1/1295996‑1296443                                                                                                                                                                                                                                           |                                                                                                                                                                                                                      aaaTAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGcc                                                                                                                                                                                                       <  1:534402/251‑1 (MQ=255)      gTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTcca                                                                                                                                                                                                  >  1:146265/1‑249 (MQ=255)         cAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTccagta                                                                                                                                                                                               >  1:147731/1‑246 (MQ=255)             tatTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACaaa                                                                                                                                                                                           >  1:372098/1‑251 (MQ=255)                   gCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCtt                                                                                                                                                                                      >  1:102854/1‑250 (MQ=255)                        aTCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCt                                                                                                                                                                                 >  1:598807/1‑250 (MQ=255)                        aTCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCt                                                                                                                                                                                 >  1:755772/1‑250 (MQ=255)                                  tcGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCtt                                                                                                                                                                                                                 >  1:194359/1‑208 (MQ=255)                                           ggCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAAt                                                                                                                                                              >  1:765734/1‑250 (MQ=255)                                               tAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAAc                                                                                                                                                         <  1:604737/251‑1 (MQ=255)                                                aGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACg                                                                                                                                                        >  1:853795/1‑251 (MQ=255)                                                         aCGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCt                                                                                                                                               <  1:153904/251‑1 (MQ=255)                                                                ggCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTg                                                                                                                                        >  1:104007/1‑251 (MQ=255)                                                                            ggCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCgaga                                                                                                                             >  1:473612/1‑250 (MQ=255)                                                                                  ggTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAacac                                                                                                                       <  1:23451/250‑1 (MQ=255)                                                                                      ctcGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGAt                                                                                                                  <  1:813220/251‑1 (MQ=255)                                                                                      ctcGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGAt                                                                                                                  <  1:862477/251‑1 (MQ=255)                                                                                            acaTGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTcc                                                                                                            <  1:617054/251‑1 (MQ=255)                                                                                                 ccGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTc                                                                                                       <  1:68208/251‑1 (MQ=255)                                                                                                         gTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATc                                                                                                                                                             <  1:31273/189‑1 (MQ=255)                                                                                                         gTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCt                                                                                                                                          <  1:373634/208‑1 (MQ=255)                                                                                                             aaaTCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCaa                                                                                           <  1:49810/251‑1 (MQ=255)                                                                                                                 ctctACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAAc                                                                                                                                                         >  1:232723/1‑185 (MQ=255)                                                                                                                 ctctACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTc                                                                                                       <  1:372580/235‑1 (MQ=255)                                                                                                                   ctACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGc                                                                                             >  1:634013/1‑243 (MQ=255)                                                                                                                            ccGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTg                                                                                                                                        >  1:565227/1‑191 (MQ=255)                                                                                                                                           ttATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGaca                                                                                                                           >  1:679958/1‑189 (MQ=255)                                                                                                                                           ttATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGaa                                                              >  1:45851/1‑250 (MQ=255)                                                                                                                                           ttATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGaa                                                              >  1:656074/1‑250 (MQ=255)                                                                                                                                                aTCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAg                                                                <  1:65448/243‑1 (MQ=255)                                                                                                                                                aTCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAAt                                                        >  1:832235/1‑251 (MQ=255)                                                                                                                                                      tgctgcGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGAt                                                  <  1:350230/251‑1 (MQ=255)                                                                                                                                                              cAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCgg                                          <  1:702551/251‑1 (MQ=255)                                                                                                                                                                aTCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTg                                        <  1:772387/251‑1 (MQ=255)                                                                                                                                                                 tctcTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTcc                                                                                                            <  1:23072/182‑1 (MQ=255)                                                                                                                                                                  ctctAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTc                                                                                                       >  1:663032/1‑186 (MQ=255)                                                                                                                                                                  ctctAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAg                                      >  1:152494/1‑251 (MQ=255)                                                                                                                                                                  ctctAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAg                                      >  1:265119/1‑251 (MQ=255)                                                                                                                                                                         tctggttcGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCg                                 <  1:116601/242‑1 (MQ=255)                                                                                                                                                                            ggttcGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAacac                                                                                                                       <  1:33126/156‑1 (MQ=255)                                                                                                                                                                                  gACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAgg                                                                                                               >  1:448139/1‑162 (MQ=255)                                                                                                                                                                                    cTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACAcc                     <  1:755870/250‑1 (MQ=255)                                                                                                                                                                                      cATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGc                                                                                             >  1:837863/1‑176 (MQ=255)                                                                                                                                                                                      cATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAg                   >  1:788769/1‑250 (MQ=255)                                                                                                                                                                                            aaCCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTacac            <  1:488132/251‑1 (MQ=255)                                                                                                                                                                                            aaCCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTacac            <  1:822461/251‑1 (MQ=255)                                                                                                                                                                                            aaCCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTacac            <  1:535062/251‑1 (MQ=255)                                                                                                                                                                                                     aaTCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATcccc    >  1:484687/1‑250 (MQ=255)                                                                                                                                                                                                     aaTCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATcccc    >  1:417761/1‑250 (MQ=255)                                                                                                                                                                                                      aTCATGACATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATCCCCCg  >  1:350783/1‑251 (MQ=255)                                                                                                                                                                                                             cATTTAAACGTTTTTAATTTTTTAAGCAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGa                                                                                        <  1:513087/158‑1 (MQ=255)                                                                                                                                                                                                                                       cAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGc                                                                                             <  1:363/127‑1 (MQ=255)                                                                                                                                                                                                                                       cAAATAAGCTTTGTTTCCGCCTTCCAGTACAAAAGCTTCGCCTCTCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGAc                                                                                       >  1:678024/1‑133 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                      AAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTAATTTTTTAAGCAAAAAAGCTTTGTTTCCGCCTTCGAGCACAAAAGCTTCGCCTCCCAGCGCCCGATCTAAATCAAACGGTTCGGGCTTGTCTTGCCAAAGCGAGACAACACATGATAGGTCCTGGTCAGTCCACTGCAACGACCAATCGTCACCTCTGGTGATAAGAACCAAATACGGATTATCTCGGTGAGTTTCGCCCACTAACACCAGTTTACACTTATCCCCCG  >  1/1295996‑1296443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑