Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,987	C→T	G156D (GGT→GAT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,987	0	C	T	100.0%	116.9 / NA	40	G156D (GGT→GAT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/22);  total (18/22)

GTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATTTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGATAATGCTCTTTGGCTCTTACGCTTTTTACGAGCAGCTTTTGGTTTAGACCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTAATTTTTTAAGCAAAA  >  1/1295740‑1296230                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatccg                                                                                                                                                                                                                                                  >  1:267404/1‑251 (MQ=255)                     aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                                              <  1:243246/251‑1 (MQ=255)                     aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAata                                                                                                                                                                                                                               <  1:635794/250‑1 (MQ=255)                     aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAata                                                                                                                                                                                                                               <  1:28989/250‑1 (MQ=255)                            ccAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                                       <  1:488604/251‑1 (MQ=255)                                  tataAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTAc                                                                                                                                                                                                                                    <  1:385155/232‑1 (MQ=255)                                      aaaTATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTAt                                                                                                                                                                                                             <  1:755179/251‑1 (MQ=255)                                                         tttCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                                          <  1:46432/251‑1 (MQ=255)                                                            ctcCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAAt                                                                                                                                                                                       >  1:379309/1‑251 (MQ=255)                                                                 gTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                                              >  1:862557/1‑207 (MQ=255)                                                                   tttGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTTGTAGATTGGCTTAGGCAATTCACGTa                                                                                                                                                                                <  1:627747/251‑1 (MQ=255)                                                                                        cACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTgg                                                                                                                                                           >  1:556550/1‑251 (MQ=255)                                                                                          cTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGt                                                                                                                                                         >  1:10239/1‑251 (MQ=255)                                                                                                       ttgttACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGc                                                                                                                                            <  1:726078/251‑1 (MQ=255)                                                                                                                 tGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                                          <  1:826788/195‑1 (MQ=255)                                                                                                                       cGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                                            <  1:808075/251‑1 (MQ=255)                                                                                                                        gTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAt                                                                                                                             >  1:81688/1‑249 (MQ=255)                                                                                                                                gTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                                            <  1:407956/242‑1 (MQ=255)                                                                                                                                     cATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                                       >  1:157972/1‑146 (MQ=255)                                                                                                                                       tctcAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                                          <  1:109294/173‑1 (MQ=255)                                                                                                                                                    gATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTtat                                                                                               >  1:14641/1‑251 (MQ=255)                                                                                                                                                             gtcTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCAtg                                                                                      >  1:131122/1‑251 (MQ=255)                                                                                                                                                               cTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                                              <  1:671907/113‑1 (MQ=255)                                                                                                                                                                       ttGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAAt                                                                            >  1:865293/1‑251 (MQ=255)                                                                                                                                                                             tatCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTaa                                                                      >  1:538723/1‑251 (MQ=255)                                                                                                                                                                              atCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                                       >  1:212009/1‑105 (MQ=255)                                                                                                                                                                              atCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTACCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAg                                                                     <  1:522719/251‑1 (MQ=255)                                                                                                                                                                                cTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGc                                                                                                                                                               <  1:286575/159‑1 (MQ=255)                                                                                                                                                                                        ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCg                                                                                                               <  1:264940/199‑1 (MQ=255)                                                                                                                                                                                        ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTc                                                                   >  1:551755/1‑243 (MQ=255)                                                                                                                                                                                        ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttcgg                                                           >  1:665011/1‑246 (MQ=255)                                                                                                                                                                                        ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttcgg                                                           >  1:324814/1‑246 (MQ=255)                                                                                                                                                                                        ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttc                                                             <  1:126818/249‑4 (MQ=255)                                                                                                                                                                                                     tggtAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAAcc                                              <  1:745079/248‑1 (MQ=255)                                                                                                                                                                                                       gtAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACAATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTa                                                                                                                                                                                >  1:284855/2‑119 (MQ=255)                                                                                                                                                                                                             ctctTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAAt                                      >  1:107940/1‑251 (MQ=255)                                                                                                                                                                                                                       ctTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                                            <  1:350986/155‑1 (MQ=255)                                                                                                                                                                                                                             cTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGt                      <  1:237657/251‑1 (MQ=255)                                                                                                                                                                                                                               tgggaatcgcagcTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACg                       <  1:843394/240‑1 (MQ=255)                                                                                                                                                                                                                                  taccagcagcTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTa                 >  1:386965/5‑251 (MQ=255)                                                                                                                                                                                                                                                 gcttAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTAATTTTTTAAGCaaat  >  1:214340/3‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATTTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGATAATGCTCTTTGGCTCTTACGCTTTTTACGAGCAGCTTTTGGTTTAGACCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTAATTTTTTAAGCAAAA  >  1/1295740‑1296230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑