Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,981	T→C	N158S (AAC→AGC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,981	0	T	C	100.0%	145.9 / NA	49	N158S (AAC→AGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

CCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATTTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGATAATGCTCTTTGGCTCTTACGCTTTTTACGAGCAGCTTTTGGTTTAGACCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTA  >  1/1295738‑1296215                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:861604/1‑249 (MQ=255) ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:604989/1‑249 (MQ=255) ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:527903/1‑249 (MQ=255) ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:154325/1‑249 (MQ=255) ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:239978/1‑249 (MQ=255)   gTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatccg                                                                                                                                                                                                                                   >  1:267404/1‑251 (MQ=255)                       aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                               <  1:243246/251‑1 (MQ=255)                       aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAata                                                                                                                                                                                                                <  1:635794/250‑1 (MQ=255)                       aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAata                                                                                                                                                                                                                <  1:28989/250‑1 (MQ=255)                              ccAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                        <  1:488604/251‑1 (MQ=255)                                  gATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatc                                                                                                                                                                                                                                     >  1:32459/1‑216 (MQ=255)                                    tataAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTAc                                                                                                                                                                                                                     <  1:385155/232‑1 (MQ=255)                                        aaaTATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTAt                                                                                                                                                                                              <  1:755179/251‑1 (MQ=255)                                            atTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatc                                                                                                                                                                                                                                     <  1:90260/208‑3 (MQ=255)                                                           tttCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                           <  1:46432/251‑1 (MQ=255)                                                              ctcCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAAt                                                                                                                                                                        >  1:379309/1‑251 (MQ=255)                                                                   gTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                               >  1:862557/1‑207 (MQ=255)                                                                     tttGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTTGTAGATTGGCTTAGGCAATTCACGTa                                                                                                                                                                 <  1:627747/251‑1 (MQ=255)                                                                                          cACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTgg                                                                                                                                            >  1:556550/1‑251 (MQ=255)                                                                                            cTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGt                                                                                                                                          >  1:10239/1‑251 (MQ=255)                                                                                                         ttgttACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGc                                                                                                                             <  1:726078/251‑1 (MQ=255)                                                                                                                 ctTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:157730/1‑137 (MQ=255)                                                                                                                   tGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                           <  1:826788/195‑1 (MQ=255)                                                                                                                        ccGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatc                                                                                                                                                                                                                                     >  1:300960/1‑130 (MQ=255)                                                                                                                         cGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                             <  1:808075/251‑1 (MQ=255)                                                                                                                          gTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAt                                                                                                              >  1:81688/1‑249 (MQ=255)                                                                                                                                  gTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                             <  1:407956/242‑1 (MQ=255)                                                                                                                                       cATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                        >  1:157972/1‑146 (MQ=255)                                                                                                                                         tctcAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                           <  1:109294/173‑1 (MQ=255)                                                                                                                                                      gATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTtat                                                                                >  1:14641/1‑251 (MQ=255)                                                                                                                                                               gtcTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCAtg                                                                       >  1:131122/1‑251 (MQ=255)                                                                                                                                                                 cTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                               <  1:671907/113‑1 (MQ=255)                                                                                                                                                                         ttGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAAt                                                             >  1:865293/1‑251 (MQ=255)                                                                                                                                                                               tatCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTaa                                                       >  1:538723/1‑251 (MQ=255)                                                                                                                                                                                atCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                        >  1:212009/1‑105 (MQ=255)                                                                                                                                                                                atCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTACCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAg                                                      <  1:522719/251‑1 (MQ=255)                                                                                                                                                                                  cTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGc                                                                                                                                                <  1:286575/159‑1 (MQ=255)                                                                                                                                                                                          ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCg                                                                                                <  1:264940/199‑1 (MQ=255)                                                                                                                                                                                          ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTc                                                    >  1:551755/1‑243 (MQ=255)                                                                                                                                                                                          ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttcgg                                            >  1:665011/1‑246 (MQ=255)                                                                                                                                                                                          ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttcgg                                            >  1:324814/1‑246 (MQ=255)                                                                                                                                                                                          ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttc                                              <  1:126818/249‑4 (MQ=255)                                                                                                                                                                                                       tggtAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAAcc                               <  1:745079/248‑1 (MQ=255)                                                                                                                                                                                                         gtAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACAATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTa                                                                                                                                                                 >  1:284855/2‑119 (MQ=255)                                                                                                                                                                                                               ctctTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAAt                       >  1:107940/1‑251 (MQ=255)                                                                                                                                                                                                                         ctTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                             <  1:350986/155‑1 (MQ=255)                                                                                                                                                                                                                               cTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGt       <  1:237657/251‑1 (MQ=255)                                                                                                                                                                                                                                 tgggaatcgcagcTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACg        <  1:843394/240‑1 (MQ=255)                                                                                                                                                                                                                                    taccagcagcTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTa  >  1:386965/5‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           CCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATTTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGATAATGCTCTTTGGCTCTTACGCTTTTTACGAGCAGCTTTTGGTTTAGACCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTA  >  1/1295738‑1296215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑