Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,977	T→C	Q159Q (CAA→CAG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,977	0	T	C	100.0%	164.7 / NA	55	Q159Q (CAA→CAG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

GATATACACCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATTTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGATAATGCTCTTTGGCTCTTACGCTTTTTACGAGCAGCTTTTGGTTTAGACCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTA  >  1/1295730‑1296215                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gATATACACCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTgg                                                                                                                                                                                                                                             >  1:425696/1‑251 (MQ=255) gATATACACCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTgg                                                                                                                                                                                                                                             >  1:589677/1‑251 (MQ=255) gATATACACCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGAAATGCTCTTTGTCTCTTACGCTTTTTACCAGCAGCTTCTgg                                                                                                                                                                                                                                             >  1:16559/1‑251 (MQ=255)   tataCACCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTggct                                                                                                                                                                                                                                           <  1:642602/251‑3 (MQ=255)         ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:861604/1‑249 (MQ=255)         ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:604989/1‑249 (MQ=255)         ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:154325/1‑249 (MQ=255)         ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:527903/1‑249 (MQ=255)         ccGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:239978/1‑249 (MQ=255)           gTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatccg                                                                                                                                                                                                                                   >  1:267404/1‑251 (MQ=255)                               aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                               <  1:243246/251‑1 (MQ=255)                               aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAata                                                                                                                                                                                                                <  1:28989/250‑1 (MQ=255)                               aaCATAACCAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAata                                                                                                                                                                                                                <  1:635794/250‑1 (MQ=255)                                      ccAAGATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                        <  1:488604/251‑1 (MQ=255)                                          gATATAAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatc                                                                                                                                                                                                                                     >  1:32459/1‑216 (MQ=255)                                            tataAAATATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTAc                                                                                                                                                                                                                     <  1:385155/232‑1 (MQ=255)                                                aaaTATTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTAt                                                                                                                                                                                              <  1:755179/251‑1 (MQ=255)                                                    atTTTAACATCTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatc                                                                                                                                                                                                                                     <  1:90260/208‑3 (MQ=255)                                                                   tttCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                           <  1:46432/251‑1 (MQ=255)                                                                      ctcCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAAt                                                                                                                                                                        >  1:379309/1‑251 (MQ=255)                                                                           gTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                               >  1:862557/1‑207 (MQ=255)                                                                             tttGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTTGTAGATTGGCTTAGGCAATTCACGTa                                                                                                                                                                 <  1:627747/251‑1 (MQ=255)                                                                                                  cACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTgg                                                                                                                                            >  1:556550/1‑251 (MQ=255)                                                                                                    cTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGt                                                                                                                                          >  1:10239/1‑251 (MQ=255)                                                                                                            gTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTgg                                                                                                                                                                                                                                             <  1:299320/144‑1 (MQ=255)                                                                                                                 ttgttACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGc                                                                                                                             <  1:726078/251‑1 (MQ=255)                                                                                                                       ctctTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTggct                                                                                                                                                                                                                                           >  1:177439/1‑133 (MQ=255)                                                                                                                         ctTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgat                                                                                                                                                                                                                                      >  1:157730/1‑137 (MQ=255)                                                                                                                           tGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                           <  1:826788/195‑1 (MQ=255)                                                                                                                                ccGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAgatc                                                                                                                                                                                                                                     >  1:300960/1‑130 (MQ=255)                                                                                                                                 cGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                             <  1:808075/251‑1 (MQ=255)                                                                                                                                  gTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAt                                                                                                              >  1:81688/1‑249 (MQ=255)                                                                                                                                          gTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                             <  1:407956/242‑1 (MQ=255)                                                                                                                                               cATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                        >  1:157972/1‑146 (MQ=255)                                                                                                                                                 tctcAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGc                                                                                                                                                                           <  1:109294/173‑1 (MQ=255)                                                                                                                                                              gATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTtat                                                                                >  1:14641/1‑251 (MQ=255)                                                                                                                                                                       gtcTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCAtg                                                                       >  1:131122/1‑251 (MQ=255)                                                                                                                                                                         cTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAatat                                                                                                                                                                                                               <  1:671907/113‑1 (MQ=255)                                                                                                                                                                                 ttGTAATATCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAAt                                                             >  1:865293/1‑251 (MQ=255)                                                                                                                                                                                       tatCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTaa                                                       >  1:538723/1‑251 (MQ=255)                                                                                                                                                                                        atCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCAc                                                                                                                                                                                                        >  1:212009/1‑105 (MQ=255)                                                                                                                                                                                        atCTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTACCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAg                                                      <  1:522719/251‑1 (MQ=255)                                                                                                                                                                                          cTGCCATGCTCTAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGc                                                                                                                                                <  1:286575/159‑1 (MQ=255)                                                                                                                                                                                                  ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCg                                                                                                <  1:264940/199‑1 (MQ=255)                                                                                                                                                                                                  ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTc                                                    >  1:551755/1‑243 (MQ=255)                                                                                                                                                                                                  ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttcgg                                            >  1:665011/1‑246 (MQ=255)                                                                                                                                                                                                  ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttcgg                                            >  1:324814/1‑246 (MQ=255)                                                                                                                                                                                                  ctctAGTGCCGAGTGGTAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTggttc                                              <  1:126818/249‑4 (MQ=255)                                                                                                                                                                                                               tggtAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAAcc                               <  1:745079/248‑1 (MQ=255)                                                                                                                                                                                                                 gtAATGCTCTTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACAATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTa                                                                                                                                                                 >  1:284855/2‑119 (MQ=255)                                                                                                                                                                                                                       ctctTTGGCTCTTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAAt                       >  1:107940/1‑251 (MQ=255)                                                                                                                                                                                                                                 ctTACGCTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAAtc                                                                                                             <  1:350986/155‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTTTACCAGCAGCTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGt       <  1:237657/251‑1 (MQ=255)                                                                                                                                                                                                                                         tgggaatcgcagcTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACg        <  1:843394/240‑1 (MQ=255)                                                                                                                                                                                                                                            taccagcagcTTCTGGCTTAGATCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGTTCGGACTCATAATAACCAAGTAAATCATGACATTTAAACGTTTTTa  >  1:386965/5‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GATATACACCGTTAAGTGGATAGATAAAAGAACATAACCAAGATATAAAATATTTTAACATTTGAATTTCTCCTGTTTTGATAAAAATAAAAAACCGCACTTTCGCGGTCTGTTGTTACTCTTGATACCGTTTACCAGTTAACATCTCAATAACTTGGATTACGTCGTCTTTTGATTTGTAATATCTGCCATGCTCTAGTGCCGAGTGATAATGCTCTTTGGCTCTTACGCTTTTTACGAGCAGCTTTTGGTTTAGACCCGACCCCAAATAGTACAAATATTCTGCACCATCCGGTATCTCGTAGATTGGCTTAGGCAATTCACGTAAAGGCGCCTTAATTGGCTTGGGTTCTCGCCACATGCCGACAATGTCTAAATCTCTACGACTACCGTCAAAATTACCTTTATAATCCCATGCTGCGGCAATCTCTAAGTCTGGACCGGACTCATAATAACCAAGTAAATCATGACATTTGAACGTTTTTA  >  1/1295730‑1296215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑