Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,295,009 A→G intergenic (+259/+242) unknown → / ← unknown hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,295,0090AG100.0% 76.2 / NA 26intergenic (+259/+242)unknown/unknownhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

AGCCAGAGTTAGTCAGTTTTTATTTTAAGGGAAAAGGCTGCTTAAATTAGTCCTAGTAGCTACGTGAATTAGCTTGCTTCACTAGGAAGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTAATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGATATAATGCAATGATTTATAAATGAATAAAAATGATATTGTTTATTC  >  1/1294765‑1295212
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                           
aGCCAGAGTTAGTCAGTTTTTATTTTAAGGGAAAAGGCTGCTTAAATTAGTCCTAGTAGCTACGTGAATTAGCTTGCTTCACTAGGAAGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAg                                                                                                                                                                                                       >  1:103968/1‑251 (MQ=255)
  ccAGAGTTAGTCAGTTTTTATTTTAAGGGAAAAGGCTGCTTAAATTAGTCCTAGTAGCTACGTGAATTAGCTTGCTTCACTAGGAAGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGAt                                                                                                                                                                                                     <  1:127503/251‑1 (MQ=255)
                                            aaTTAGTCCTAGTAGCTACGTGAATTAGCTTGCTTCACTAGGAAGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGatat                                                                                                                                                                                                         <  1:231055/205‑1 (MQ=255)
                                                aGTCCTAGTAGCTACGTGAATTAGCTTGCTTCACTAGGAAGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACtt                                                                                                                                                       >  1:886810/1‑251 (MQ=255)
                                                           cTACGTGAATTAGCTTGCTTCACTAGGAAGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTa                                                                                                                                                  >  1:49961/1‑245 (MQ=255)
                                                                                       aGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACAt                                                                                                                                                                             >  1:698721/1‑190 (MQ=255)
                                                                                                   atatAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAg                                                                                                                                                                                                       <  1:372505/152‑1 (MQ=255)
                                                                                                     ataAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAAc                                                                                                  <  1:582636/251‑1 (MQ=255)
                                                                                                            aaaaaaTGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAAt                                                                                           <  1:880451/251‑1 (MQ=255)
                                                                                                            aaaaaaTGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAAt                                                                                           >  1:691625/1‑251 (MQ=255)
                                                                                                             aaaaaTGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAAtt                                                                                          >  1:23009/1‑251 (MQ=255)
                                                                                                              aaaaTGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAg                                                                                                                                                                                                       <  1:158802/141‑1 (MQ=255)
                                                                                                                                                    cGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACg                                                                                                                                       <  1:392674/167‑1 (MQ=255)
                                                                                                                                                    cGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAAc                                                                                                                <  1:520075/190‑1 (MQ=255)
                                                                                                                                                    cGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCgg                                                   >  1:579898/1‑251 (MQ=255)
                                                                                                                                                                        atataAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGCGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTta                               >  1:606548/1‑251 (MQ=255)
                                                                                                                                                                        atataAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGAt                                                                                                                                                                                                     >  1:448255/1‑85 (MQ=255)
                                                                                                                                                                        atataAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGattat                                                          <  1:418394/224‑1 (MQ=255)
                                                                                                                                                                                                     ttAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGACATAATGCAATGATTTATCAATGAATAAAATTGATATTGTTTATtc  <  1:282296/251‑1 (MQ=255)
                                                                                                                                                                                                         gtgtTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTg                                                                                                                                                                          >  1:617514/1‑79 (MQ=255)
                                                                                                                                                                                                         gtgtTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTa                                                                                                                                     <  1:598567/116‑1 (MQ=255)
                                                                                                                                                                                                           gtTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATta                                                                                                                        >  1:243579/1‑127 (MQ=255)
                                                                                                                                                                                                                         tatCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGa                                                                                                                                                                         <  1:851995/64‑1 (MQ=255)
                                                                                                                                                                                                                           tCTGATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCAc                                                                  <  1:815303/165‑1 (MQ=255)
                                                                                                                                                                                                                              gATGGCACTGGAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTa                                                                        >  1:649543/1‑156 (MQ=255)
                                                                                                                                                                                                                                        gAGTAAAAACCTGATATAGATCGTGTGTTGAACGATGTTGTACATTTg                                                                                                                                                                          <  1:530045/48‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                           
AGCCAGAGTTAGTCAGTTTTTATTTTAAGGGAAAAGGCTGCTTAAATTAGTCCTAGTAGCTACGTGAATTAGCTTGCTTCACTAGGAAGGTAGTTGTAAATATAAGTGAAAAAATGCATCAATTCAGAATGAAGTGGTACCCTGCTAACGATAGAAGCTTAATTTGATATATAAGATTGTTCGCTTATAAAAAATTTTTAGGTGTTAGAACCTTAGATATCTGATGGCACTGGAGTAAAAACCTAATATAGATCGTGTGTTGAACGATGTTGTACATTTGATGCTAAAGTGCCCTACTTGGGTATGCTAATTACGTAAGAGCGGTAATTATATAAAACGAATTTAAATAAACAGAAAATTATATGAAATGGGGAAGTATCCCACTCGATTATTTATCGGCGATATAATGCAATGATTTATAAATGAATAAAAATGATATTGTTTATTC  >  1/1294765‑1295212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑