Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,284,605	A→G	F243F (TTT→TTC)	unknown ←	AmpG permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,284,605	0	A	G	100.0%	169.1 / NA	59	F243F (TTT→TTC)	unknown	AmpG permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/27);  total (32/27)

AGGTAATTTGTGCAAAATGCCCAAAAGCGGCAAGCCAAACGAATCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCAAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAGGGTGGCGAGATAAAGGGAAAGCCCGCCGGGA  >  1/1284358‑1284851                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aGGTAATTTGTGCAAAATGCCCAAAAGCGGCAAGCCAAACGAATCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATAC‑TGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAAtt                                                                                                                                                                                                                                                    >  1:159073/1‑251 (MQ=255)  ggTAATTTGTGCAAAATGCCCAAAAGCGGCAAGCCAAACGAATCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAAtt                                                                                                                                                                                                                                                    >  1:330024/1‑251 (MQ=255)                 aTGCCCAAAAGCGGCAAGCCAAACGAATCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGaa                                                                                                                                                                                                                                     >  1:603292/1‑251 (MQ=255)                               cAAGCCAAACGAATCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGcc                                                                                                                                                                                                                        <  1:129291/250‑1 (MQ=255)                                          aaTCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGtat                                                                                                                                                                                                            <  1:578295/251‑1 (MQ=255)                                          aaTCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGtat                                                                                                                                                                                                            <  1:879185/251‑1 (MQ=255)                                              cgccgATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACg                                                                                                                                                                                                        >  1:101549/1‑251 (MQ=255)                                                ccgATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGc                                                                                                                                                                                                       <  1:855906/250‑1 (MQ=255)                                                      gTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTAc                                                                                                                                                                                                 <  1:308206/250‑1 (MQ=255)                                                             aTTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGaaa                                                                                                                                                                                         >  1:694146/1‑251 (MQ=255)                                                                gTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAAt                                                                                                                                                                                      >  1:669183/1‑251 (MQ=255)                                                                gTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTAGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAAt                                                                                                                                                                                      >  1:432221/1‑251 (MQ=255)                                                                     aaaCCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTg                                                                                                                                                                                 >  1:340599/1‑251 (MQ=255)                                                                      aaCCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTgg                                                                                                                                                                                >  1:772351/1‑251 (MQ=255)                                                                       aCCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATaa                                                                                                                                                                                                          >  1:63669/1‑224 (MQ=255)                                                                       aCCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGc                                                                                                                                                                                                       >  1:821990/1‑227 (MQ=255)                                                                             gACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGaa                                                                                                                                                                         >  1:681291/1‑251 (MQ=255)                                                                               cccATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGc                                                                                                                                                                                                                         <  1:227081/201‑1 (MQ=255)                                                                                                           aaTCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCaaa                                                                                                                                                                   >  1:892724/1‑227 (MQ=255)                                                                                                            aTCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGt                                                                                                                                                                                               <  1:376718/198‑1 (MQ=255)                                                                                                                 aaTCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAAc                                                                                                                                                                                                                                         >  1:94155/1‑151 (MQ=255)                                                                                                                   tCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACg                                                                                                                                   <  1:697479/251‑1 (MQ=255)                                                                                                                          aCCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATAT‑CTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATa                                                                                                                                                         >  1:5788/1‑221 (MQ=255)                                                                                                                            ccGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAAtt                                                                                                                          <  1:825374/251‑1 (MQ=255)                                                                                                                                       gCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATgg                                                                                                                               <  1:733565/235‑1 (MQ=255)                                                                                                                                           aaaTACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCg                                                                                                           <  1:285080/251‑1 (MQ=255)                                                                                                                                              tACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGcc                                                                                                                                                                                                      >  1:721548/1‑157 (MQ=255)                                                                                                                                                   gCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACaa                                                                                                   >  1:870364/1‑251 (MQ=255)                                                                                                                                                   gCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACaa                                                                                                   >  1:540175/1‑251 (MQ=255)                                                                                                                                                             aaGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAAc                                                                                         <  1:862830/251‑1 (MQ=255)                                                                                                                                                             aaGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAAc                                                                                         <  1:463026/251‑1 (MQ=255)                                                                                                                                                             aaGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAAc                                                                                         <  1:614278/251‑1 (MQ=255)                                                                                                                                                                        aCAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAg                                                                              <  1:876477/251‑1 (MQ=255)                                                                                                                                                                         cAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAg                                                                              >  1:219681/1‑250 (MQ=255)                                                                                                                                                                          aaCTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCa                                                                            >  1:78642/1‑251 (MQ=255)                                                                                                                                                                            cTAGGGCAATATCCACTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGtat                                                                                                                                                                                                            >  1:77991/1‑121 (MQ=255)                                                                                                                                                                                    atatCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCg                                                                                                                                                    <  1:72452/169‑1 (MQ=255)                                                                                                                                                                                      atCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACaaa                                                                      >  1:482209/1‑245 (MQ=255)                                                                                                                                                                                              tGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATaaa                                                        <  1:620225/251‑1 (MQ=255)                                                                                                                                                                                              tGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATaaa                                                        <  1:642656/251‑1 (MQ=255)                                                                                                                                                                                               gCTGAATCCTTTGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTc                                                                                                                  <  1:514627/192‑1 (MQ=255)                                                                                                                                                                                               gCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGc                                                                                                                                                                                                       >  1:101444/1‑107 (MQ=255)                                                                                                                                                                                               gCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATaaaa                                                       >  1:138450/1‑251 (MQ=255)                                                                                                                                                                                                    aTCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGcc                                                                                                                                                                                                                 >  1:711687/1‑92 (MQ=255)                                                                                                                                                                                                    aTCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGcc                                                                                                                                                     >  1:573543/1‑152 (MQ=255)                                                                                                                                                                                                    aTCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAAc                                                   >  1:530949/1‑250 (MQ=255)                                                                                                                                                                                                     tCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCg                                                 <  1:488966/251‑1 (MQ=255)                                                                                                                                                                                                                aaataaatTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAgggg                                      <  1:546591/251‑1 (MQ=255)                                                                                                                                                                                                                   taaatTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTaa                                   >  1:180638/1‑251 (MQ=255)                                                                                                                                                                                                                     aatTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAg                                 <  1:126674/251‑1 (MQ=255)                                                                                                                                                                                                                     aatTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAg                                 >  1:35781/1‑251 (MQ=255)                                                                                                                                                                                                                       tttGGTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAggg                               <  1:794647/251‑1 (MQ=255)                                                                                                                                                                                                                          ggTTTGTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAg                                                                     <  1:127459/210‑1 (MQ=255)                                                                                                                                                                                                                               gTAGCGTGGTGGCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAGGGTGGCgaga                       >  1:215764/1‑251 (MQ=255)                                                                                                                                                                                                                                       gtggCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAGGGTGGCGAGATAAAggg                <  1:581664/250‑1 (MQ=255)                                                                                                                                                                                                                                       gtggCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAGGGTGGCGAGATAAAggg                <  1:537446/250‑1 (MQ=255)                                                                                                                                                                                                                                        tggCGAAGGAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAGGGTGGCGAGATAAAGGGa               <  1:633545/250‑1 (MQ=255)                                                                                                                                                                                                                                               ggAATCCCCGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAggg                               >  1:370883/1‑227 (MQ=255)                                                                                                                                                                                                                                                    ccccGAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAGGGTGGCGAGATAAAGGGAAAGCCCGCCGGGa  >  1:652076/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AGGTAATTTGTGCAAAATGCCCAAAAGCGGCAAGCCAAACGAATCCGCCGATGGTGATTAATTGTACGAAACCGAAGACCCATAAGGCGCGATTAATGCCGAGTCGAATCATAATCACGCCACCCGCGAGACCAGCGGAAATACTTGCCCAGAGGGAAGTGCTTTTGACAACTAGGGCAATATCCTCTTTGCTGAATCCTATGTCATAAATAAATTTGGTTTGTAGCGTGGTGGCGAAGGAATCCCCAAATTTATATAAAAACAGAAAGAGCAAAAAGCCCAATGCCTGTATAACGCCTTTACGTTGGAAAAATTCTTGGAGCGGAACCCAAAAGGCAAGATAAGCCGGTTGATCGCGGTTTACGATGGTAATTTGTGGTTCTTTGGCGAGAAACAAGGTCATAAACAAGCCGAACAGCATACAAAGTGCGGTCCATAAAAAAACCGTTTCCCAGGGGTAAAGGGTGGCGAGATAAAGGGAAAGCCCGCCGGGA  >  1/1284358‑1284851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑