Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	93,428	C→A	V148V (GTG→GTT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	93,428	0	C	A	100.0%	166.5 / NA	59	V148V (GTG→GTT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/32);  total (27/32)

AGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTGCACTTTTACCCAAAAAATTAAAGGTAACCACACAATCAATGGTGGGCGCGCCTTGCATGCCGACGTTAACCGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCCACAACAGACACCGATAAAAGTTTGACCGCGCTGCGGTTGGCGTTCCAAATTTGCACGTTAAAGGTCTGGTCGGTAGAGATTGCACCCAGATTTACCGTGTGCGGGATAACCACAATGCGGCGATATAAATCA  >  1/93183‑93674                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACaacac                                                                                                                                                                                                                                                    <  1:545443/250‑1 (MQ=255)   cgccgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaat                                                                                                                                                                                                                                                 <  1:190192/251‑1 (MQ=255)     ccgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaatca                                                                                                                                                                                                                                               <  1:25156/251‑1 (MQ=255)      cgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaatcaa                                                                                                                                                                                                                                              <  1:507922/251‑1 (MQ=255)         ttCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtgg                                                                                                                                                                                                                                           <  1:396453/251‑1 (MQ=255)            aaaGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtggtgg                                                                                                                                                                                                                                        <  1:684616/251‑1 (MQ=255)                         tCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCAt                                                                                                                                                                                                                           <  1:83562/251‑1 (MQ=255)                            aCATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGcc                                                                                                                                                                                                                        <  1:671204/251‑1 (MQ=255)                            aCATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGcc                                                                                                                                                                                                                        <  1:479769/251‑1 (MQ=255)                                     aaaCTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAGTGGTGGGCGAGCCCTGCATGCCGACGTTAAc                                                                                                                                                                                                               <  1:18224/251‑1 (MQ=255)                                           acttTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTggg                                                                                                                                                                                                                                       <  1:547628/221‑1 (MQ=255)                                                     cAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAg                                                                                                                                                                                                >  1:553731/1‑250 (MQ=255)                                                     cAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAg                                                                                                                                                                                                >  1:743282/1‑250 (MQ=255)                                                         cgTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtgg                                                                                                                                                                                                                                           >  1:173771/2‑203 (MQ=255)                                                               gcgcggcgACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtggtg                                                                                                                                                                                                                                         >  1:350008/1‑199 (MQ=255)                                                                      gACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAatat                                                                                                                                                                              >  1:748436/1‑251 (MQ=255)                                                                                    aaTGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGcc                                                                                                                                                                <  1:56647/251‑1 (MQ=255)                                                                                    aaTGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGcc                                                                                                                                                                <  1:689940/251‑1 (MQ=255)                                                                                                                                                   cacctcttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGt                                                                                                 >  1:255976/17‑251 (MQ=255)                                                                                                                                                     cctcttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACtt                                                                                                                                                      <  1:158018/182‑1 (MQ=255)                                                                                                                                                     cctcttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTg                                                                                               >  1:292718/15‑251 (MQ=255)                                                                                                                                                        cttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTcc                                                                                                                                                                                                          >  1:596024/12‑141 (MQ=255)                                                                                                                                                        cttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCg                                                                                            >  1:702135/12‑251 (MQ=255)                                                                                                                                                               ccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCggtgctta                                                                                     >  1:823810/5‑244 (MQ=255)                                                                                                                                                                  gtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacactt                                                                                  >  1:203569/2‑246 (MQ=255)                                                                                                                                                                  gtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacact                                                                                   >  1:412288/2‑246 (MQ=255)                                                                                                                                                                   tCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacact                                                                                   <  1:218516/249‑5 (MQ=255)                                                                                                                                                                   tCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacac                                                                                    <  1:344951/248‑4 (MQ=255)                                                                                                                                                                       gcagaaatgcccAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAata                                                                                                                                                                               >  1:365112/11‑153 (MQ=255)                                                                                                                                                                                   aaTCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGTATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACgccaaagctctg                                                                  >  1:846659/1‑239 (MQ=255)                                                                                                                                                                                     tcggTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACgccaaagctctgctc                                                               <  1:546021/248‑15 (MQ=255)                                                                                                                                                                                              cgcgAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGAt                                                      <  1:653706/251‑1 (MQ=255)                                                                                                                                                                                              cgcgAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGAt                                                      <  1:188214/251‑1 (MQ=255)                                                                                                                                                                                                  aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGcc                                                                                                                                                                           >  1:470080/1‑130 (MQ=255)                                                                                                                                                                                                  aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACCACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCa                                                  >  1:446346/1‑251 (MQ=255)                                                                                                                                                                                                  aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACGCCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacac                                                                                    <  1:137836/217‑4 (MQ=255)                                                                                                                                                                                                  aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGa                                                                                                                        >  1:62171/1‑181 (MQ=255)                                                                                                                                                                                                  aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCa                                                  >  1:532341/1‑251 (MQ=255)                                                                                                                                                                                                  aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTC‑GTTGAGATTGCa                                                  >  1:366129/1‑250 (MQ=255)                                                                                                                                                                                                    ccTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACtt                                                                                                                                                      <  1:189628/149‑1 (MQ=255)                                                                                                                                                                                                    ccTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATc                                                                                                                                                  <  1:654785/153‑1 (MQ=255)                                                                                                                                                                                                       ataATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAg                                             >  1:854739/1‑251 (MQ=255)                                                                                                                                                                                                       ataATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAg                                             >  1:224009/1‑251 (MQ=255)                                                                                                                                                                                                       ataATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAg                                             >  1:405469/1‑251 (MQ=255)                                                                                                                                                                                                             cgcAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTAc                                       <  1:515260/251‑1 (MQ=255)                                                                                                                                                                                                             cgcAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTAc                                       <  1:454248/251‑1 (MQ=255)                                                                                                                                                                                                                 gggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGAt                                           >  1:458893/1‑243 (MQ=255)                                                                                                                                                                                                                 gggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTAc                                       <  1:480284/247‑1 (MQ=255)                                                                                                                                                                                                                 gggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCgta                                   >  1:178906/1‑250 (MQ=255)                                                                                                                                                                                                                  ggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCg                                     <  1:252178/248‑1 (MQ=255)                                                                                                                                                                                                                  ggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCg                                     <  1:785963/248‑1 (MQ=255)                                                                                                                                                                                                                            cTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACtt                                                                                                                                                      <  1:281525/125‑1 (MQ=255)                                                                                                                                                                                                                                             aaaGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCGTATGGGGAATAACcaggat                  >  1:124466/1‑236 (MQ=255)                                                                                                                                                                                                                                                ggTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacact                                                                                   <  1:394844/172‑5 (MQ=255)                                                                                                                                                                                                                                                ggTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACgccaaagctc                                                                    <  1:793062/187‑10 (MQ=255)                                                                                                                                                                                                                                                ggTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCGTATGGGGAATAACCAGGATGCGCCGATATAAAt    >  1:741012/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGcc                                                                                                                                                                           >  1:251034/1‑82 (MQ=255)                                                                                                                                                                                                                                                  tAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCGTATGGGGAATAACCAGGATGCGCCGATATAAATcc  <  1:347242/251‑2 (MQ=255)                                                                                                                                                                                                                                                  tAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCGTATGGGGAATAACCAGGATGCGCCGATATAAATcc  <  1:810941/251‑2 (MQ=255)                                                                                                                                                                                                                                                  tAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCGTATGGGGAATAACCAGGATGCGCCGATATAAATc   <  1:740739/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       AGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTGCACTTTTACCCAAAAAATTAAAGGTAACCACACAATCAATGGTGGGCGCGCCTTGCATGCCGACGTTAACCGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCCACAACAGACACCGATAAAAGTTTGACCGCGCTGCGGTTGGCGTTCCAAATTTGCACGTTAAAGGTCTGGTCGGTAGAGATTGCACCCAGATTTACCGTGTGCGGGATAACCACAATGCGGCGATATAAATCA  >  1/93183‑93674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑