Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	93,398	T→C	A158A (GCA→GCG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	93,398	0	T	C	100.0%	181.4 / NA	63	A158A (GCA→GCG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/30);  total (33/30)

CACACGCGCGAGCCATAACCATACAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTGCACTTTTACCCAAAAAATTAAAGGTAACCACACAATCAATGGTGGGCGCGCCTTGCATGCCGACGTTAACCGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCCACAACAGACACCGATAAAAGTTTGACCGCGCTGCGGTTGGCGTTCCAAATTTGCACGTTAAAGGTCTGGTCGGTAGAGATTGCACCCAGATTTACCGTG  >  1/93159‑93641                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    catacGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTAcc                                                                                                                                                                                                                                          <  1:550401/248‑1 (MQ=255)  atacGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTAccc                                                                                                                                                                                                                                         <  1:599167/249‑1 (MQ=255)           aGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTa                                                                                                                                                                                                                                >  1:785298/1‑251 (MQ=255)           aGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTa                                                                                                                                                                                                                                >  1:576117/1‑251 (MQ=255)               aTAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAgg                                                                                                                                                                                                                            <  1:152848/251‑1 (MQ=255)                tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGGTTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAgg                                                                                                                                                                                                                            >  1:614305/1‑250 (MQ=255)                tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAgg                                                                                                                                                                                                                            >  1:627490/1‑250 (MQ=255)                tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGt                                                                                                                                                                                                                           >  1:155892/1‑251 (MQ=255)                  aCCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTaa                                                                                                                                                                                                                         >  1:879522/1‑251 (MQ=255)                  aCCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTaa                                                                                                                                                                                                                         >  1:621698/1‑251 (MQ=255)                   ccATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTATTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTaa                                                                                                                                                                                                                         >  1:397328/1‑250 (MQ=255)                         aGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACaacac                                                                                                                                                                                                                   <  1:545443/250‑1 (MQ=255)                           cgccgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaat                                                                                                                                                                                                                <  1:190192/251‑1 (MQ=255)                             ccgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaatca                                                                                                                                                                                                              <  1:25156/251‑1 (MQ=255)                              cgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaatcaa                                                                                                                                                                                                             <  1:507922/251‑1 (MQ=255)                               gcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAAcaac                                                                                                                                                                                                                     >  1:325259/1‑239 (MQ=255)                                 ttCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtgg                                                                                                                                                                                                          <  1:396453/251‑1 (MQ=255)                                    aaaGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtggtgg                                                                                                                                                                                                       <  1:684616/251‑1 (MQ=255)                                                 tCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCAt                                                                                                                                                                                          <  1:83562/251‑1 (MQ=255)                                                    aCATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGcc                                                                                                                                                                                       <  1:671204/251‑1 (MQ=255)                                                    aCATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGcc                                                                                                                                                                                       <  1:479769/251‑1 (MQ=255)                                                             aaaCTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAGTGGTGGGCGAGCCCTGCATGCCGACGTTAAc                                                                                                                                                                              <  1:18224/251‑1 (MQ=255)                                                                   acttTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTggg                                                                                                                                                                                                      <  1:547628/221‑1 (MQ=255)                                                                             cAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAg                                                                                                                                                               >  1:553731/1‑250 (MQ=255)                                                                             cAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAg                                                                                                                                                               >  1:743282/1‑250 (MQ=255)                                                                                 cgTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtgg                                                                                                                                                                                                          >  1:173771/2‑203 (MQ=255)                                                                                       gcgcggcgACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtggtg                                                                                                                                                                                                        >  1:350008/1‑199 (MQ=255)                                                                                              gACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAatat                                                                                                                                             >  1:748436/1‑251 (MQ=255)                                                                                                            aaTGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGcc                                                                                                                               <  1:689940/251‑1 (MQ=255)                                                                                                            aaTGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGcc                                                                                                                               <  1:56647/251‑1 (MQ=255)                                                                                                                                                                           cacctcttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGt                                                                >  1:255976/17‑251 (MQ=255)                                                                                                                                                                             cctcttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACtt                                                                                                                     <  1:158018/182‑1 (MQ=255)                                                                                                                                                                             cctcttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTg                                                              >  1:292718/15‑251 (MQ=255)                                                                                                                                                                                cttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTcc                                                                                                                                                                         >  1:596024/12‑141 (MQ=255)                                                                                                                                                                                cttgtgaccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCg                                                           >  1:702135/12‑251 (MQ=255)                                                                                                                                                                                       ccagtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCggtgctta                                                    >  1:823810/5‑244 (MQ=255)                                                                                                                                                                                          gtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacactt                                                 >  1:203569/2‑246 (MQ=255)                                                                                                                                                                                          gtCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacact                                                  >  1:412288/2‑246 (MQ=255)                                                                                                                                                                                           tCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacact                                                  <  1:218516/249‑5 (MQ=255)                                                                                                                                                                                           tCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacac                                                   <  1:344951/248‑4 (MQ=255)                                                                                                                                                                                               gcagaaatgcccAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAata                                                                                                                                              >  1:365112/11‑153 (MQ=255)                                                                                                                                                                                                           aaTCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGTATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACgccaaagctctg                                 >  1:846659/1‑239 (MQ=255)                                                                                                                                                                                                             tcggTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACgccaaagctctgctc                              <  1:546021/248‑15 (MQ=255)                                                                                                                                                                                                                      cgcgAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGAt                     <  1:188214/251‑1 (MQ=255)                                                                                                                                                                                                                      cgcgAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGAt                     <  1:653706/251‑1 (MQ=255)                                                                                                                                                                                                                          aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGcc                                                                                                                                          >  1:470080/1‑130 (MQ=255)                                                                                                                                                                                                                          aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACCACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCa                 >  1:446346/1‑251 (MQ=255)                                                                                                                                                                                                                          aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACGCCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCacac                                                   <  1:137836/217‑4 (MQ=255)                                                                                                                                                                                                                          aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGa                                                                                       >  1:62171/1‑181 (MQ=255)                                                                                                                                                                                                                          aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCa                 >  1:532341/1‑251 (MQ=255)                                                                                                                                                                                                                          aGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTC‑GTTGAGATTGCa                 >  1:366129/1‑250 (MQ=255)                                                                                                                                                                                                                            ccTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACtt                                                                                                                     <  1:189628/149‑1 (MQ=255)                                                                                                                                                                                                                            ccTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATc                                                                                                                 <  1:654785/153‑1 (MQ=255)                                                                                                                                                                                                                               ataATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAg            >  1:405469/1‑251 (MQ=255)                                                                                                                                                                                                                               ataATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAg            >  1:224009/1‑251 (MQ=255)                                                                                                                                                                                                                               ataATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAg            >  1:854739/1‑251 (MQ=255)                                                                                                                                                                                                                                     cgcAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTAc      <  1:454248/251‑1 (MQ=255)                                                                                                                                                                                                                                     cgcAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTAc      <  1:515260/251‑1 (MQ=255)                                                                                                                                                                                                                                         gggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGAt          >  1:458893/1‑243 (MQ=255)                                                                                                                                                                                                                                         gggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTAc      <  1:480284/247‑1 (MQ=255)                                                                                                                                                                                                                                         gggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCgta  >  1:178906/1‑250 (MQ=255)                                                                                                                                                                                                                                          ggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCg    <  1:785963/248‑1 (MQ=255)                                                                                                                                                                                                                                          ggTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCGACAACAGACACCGATAAAAGCTTGACCGCGCTGCGGTTGGCGTTCCACACTTGCACGCCAAAGCTCTGCTCGGTTGAGATTGCACCCAGATTTACCg    <  1:252178/248‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    CACACGCGCGAGCCATAACCATACAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTGCACTTTTACCCAAAAAATTAAAGGTAACCACACAATCAATGGTGGGCGCGCCTTGCATGCCGACGTTAACCGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCCCCTCTAACTTAATCCCCTCACCACCCACAACAGACACCGATAAAAGTTTGACCGCGCTGCGGTTGGCGTTCCAAATTTGCACGTTAAAGGTCTGGTCGGTAGAGATTGCACCCAGATTTACCGTG  >  1/93159‑93641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑