Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,147,932	A→G	intergenic (+238/+66)	unknown → / ← unknown	Multidrug efflux transporter MdtK/NorM (MATE family)/Cys‑tRNA(Pro) deacylase YbaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,147,932	0	A	G	100.0%	99.9 / NA	36	intergenic (+238/+66)	unknown/unknown	Multidrug efflux transporter MdtK/NorM (MATE family)/Cys‑tRNA(Pro) deacylase YbaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

CCTGTAGGTGGGCTTGTCCCACCTTTTTTGTCTTTCGATACGATAAATCCCTCCCGCGTACATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACACAATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGATGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTC  >  1/1147696‑1148173                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  ccTGTAGGTGGGCTTGTCCCACCTTTTTTGTCTTTCGATACGATAAATCCCTCCCGCGTACATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCg                                                                                                                                                                                                                                     >  1:535821/1‑251 (MQ=255)                                             aaaTCCCTCCCGCGTACATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCAc                                                                                                                                                                                         <  1:894517/251‑1 (MQ=255)                                                         cgTACATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGa                                                                                                                                                                                                                  <  1:497538/214‑1 (MQ=255)                                                           tACATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTAccc                                                                                                                                                                            <  1:699953/250‑1 (MQ=255)                                                             cATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCt                                                                                                                                                                          >  1:869610/1‑250 (MQ=255)                                                             cATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCt                                                                                                                                                                          >  1:491812/1‑250 (MQ=255)                                                               tAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACttt                                                                                                                                                                                                                       <  1:94119/203‑1 (MQ=255)                                                                      cGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACaa                                                                                                                                                                 <  1:597303/250‑1 (MQ=255)                                                                                aaaTTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCaaaaa                                                                                                                                                       >  1:66061/1‑250 (MQ=255)                                                                                          ttttGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGa                                                                                                                                                                                                                  <  1:398151/181‑1 (MQ=255)                                                                                                aCGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCgaga                                                                                                                                                                                                                                  <  1:568622/159‑1 (MQ=255)                                                                                                     ggCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAGCTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAAc                                                                                                                                                      <  1:42371/230‑1 (MQ=255)                                                                                                         aTGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGa                                                                                                                                                                                                                  >  1:96261/1‑166 (MQ=255)                                                                                                                  gCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTAcc                                                                                                                                                                             >  1:406754/1‑194 (MQ=255)                                                                                                                               gAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAAc                                                                                                                                                      >  1:547995/1‑204 (MQ=255)                                                                                                                                    aaTCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAAt                                                                                                   >  1:439130/1‑250 (MQ=255)                                                                                                                                               tGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTAccg                                                                                       <  1:208917/251‑1 (MQ=255)                                                                                                                                                                      tGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGaa                                                                >  1:592709/1‑251 (MQ=255)                                                                                                                                                                      tGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGaa                                                                >  1:744517/1‑251 (MQ=255)                                                                                                                                                                        ttttttGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCTCTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGGTTACCGCCGGAGACGAAAATCTTGTCGAAAt                                                              <  1:240296/251‑1 (MQ=255)                                                                                                                                                                        ttttttGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATcc                                                                                                                           >  1:214558/1‑190 (MQ=255)                                                                                                                                                                            ttGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCaaaaa                                                                                                                                                       <  1:418827/158‑1 (MQ=255)                                                                                                                                                                                       gAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTc                                                            >  1:311788/1‑238 (MQ=255)                                                                                                                                                                                        aaaaaaGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTc                                              <  1:534835/251‑1 (MQ=255)                                                                                                                                                                                         aaaaaGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCa                                             <  1:849595/251‑1 (MQ=255)                                                                                                                                                                                             agagAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCaaa                                                                                                                                                         >  1:712209/1‑139 (MQ=255)                                                                                                                                                                                              gagaATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTAccc                                                                                                                                                                            <  1:322005/119‑1 (MQ=255)                                                                                                                                                                                                   tGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCaaaaa                                                                                                                                                       <  1:480467/135‑1 (MQ=255)                                                                                                                                                                                                   tGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATc                                                                        >  1:280213/1‑214 (MQ=255)                                                                                                                                                                                                    gATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAAtt                                                                                                                                                                                                              <  1:402151/79‑1 (MQ=255)                                                                                                                                                                                                    gATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCaaaaa                                                                                                                                                       <  1:868820/134‑1 (MQ=255)                                                                                                                                                                                                                              cAGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATggg         >  1:639217/1‑250 (MQ=255)                                                                                                                                                                                                                               aGCTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATggg         >  1:131147/1‑249 (MQ=255)                                                                                                                                                                                                                                 cTATACTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGc        <  1:230377/248‑1 (MQ=255)                                                                                                                                                                                                                                   ataCTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGAtt   <  1:202184/251‑1 (MQ=255)                                                                                                                                                                                                                                    taCTGACGCGATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGACGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTc  <  1:472404/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  CCTGTAGGTGGGCTTGTCCCACCTTTTTTGTCTTTCGATACGATAAATCCCTCCCGCGTACATAAAGTGCGGTCACAAAAAATTCTGTTTTTTGAACGTTGGCTATGCCAACGGCCCCGAAGGGGTGAATAAATCACTTCAGTGATTTATGAATATTTTTACAAATGTTTTTTGTTAGCTCAGAAAAAAGAGAATGATTATATTTTACGCCTGAAACTTTTCAGCTATACTGACACAATTTTTTTGACTCGAGAAAGGTAACTTTTATGAAATTTAAATTAAGTTTAATTTCCACCGCACTTTTACCCCTCATCCACAATATCCAAAAACGTCGCATTCAATATTTTCGCCAAATCCTGCGGGGCGATTTCAACGCTTAATCCGCGTTTACCGCCGGAGACGAAAATCTTGTCGAAATTCAGCGCAGATTGTTCAATGATGGTGGTTAGGCGTTTCTTTTGACCTAATGGGCTGATTC  >  1/1147696‑1148173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑