Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,135,939	A→C	E73A (GAA→GCA)	unknown →	Maltose/maltodextrin transport ATP‑binding protein MalK (EC 3.6.3.19)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,135,939	0	A	C	100.0%	215.2 / NA	75	E73A (GAA→GCA)	unknown	Maltose/maltodextrin transport ATP‑binding protein MalK (EC 3.6.3.19)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (42/33);  total (42/33)

GGTATCGAGAGGTTTCATTATGGCAAGTGTAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGAAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTGGCGATTGGGCGAACCCTTGTTTC  >  1/1135703‑1136170                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        ggTATCGAGAGGTTTCATTATGGCAAGTGTAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGcggtatcg                                                                                                                                                                                                                           >  1:299957/1‑251 (MQ=255)        agagGTTTCATTATGGCAAGTGTAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATgg                                                                                                                                                                                                                     <  1:193179/250‑1 (MQ=255)          agGTTTCATTATGGCAAGTGTAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGgt                                                                                                                                                                                                                    <  1:64239/249‑1 (MQ=255)           ggTTTCATTATGGCAAGTGTAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGcggtatcgg                                                                                                                                                                                                                          <  1:145663/242‑1 (MQ=255)           ggTTTCATTATGGCAAGTGTAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGtt                                                                                                                                                                                                                 >  1:894215/1‑251 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGcggtatcggta                                                                                                                                                                                                                        >  1:198448/1‑228 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATgg                                                                                                                                                                                                                     <  1:150607/231‑1 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATgg                                                                                                                                                                                                                     <  1:271169/231‑1 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGgtgt                                                                                                                                                                                                                  <  1:291609/234‑1 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCt                                                                                                                                                                                                          >  1:369995/1‑242 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGcc                                                                                                                                                                                                    >  1:526085/1‑248 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGcc                                                                                                                                                                                                    >  1:537272/1‑248 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGcc                                                                                                                                                                                                    >  1:782585/1‑248 (MQ=255)                           gtgtAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGcc                                                                                                                                                                                                    >  1:82842/1‑248 (MQ=255)                                    tGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGgtgt                                                                                                                                                                                                                  <  1:816662/225‑1 (MQ=255)                                    tGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCAc                                                                                                                                                                                        <  1:396522/251‑1 (MQ=255)                                     gcgcAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCa                                                                                                                                                                                         >  1:751002/1‑249 (MQ=255)                                          aTGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGcggtatcggta                                                                                                                                                                                                                        <  1:766236/213‑1 (MQ=255)                                          aTGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGcggtatcggta                                                                                                                                                                                                                        >  1:408818/1‑213 (MQ=255)                                          aTGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTAc                                                                                                                                                                                              <  1:101619/239‑1 (MQ=255)                                                    aTCTTACGGAAACGTACATATTTCCAAATCTATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGCCCGGTTGCGGTAAATCCCCCTTATTGCCAATGATCGCCGGACCTGAGGATATTACCACCGGGTCCCTTTACATCGGTTAAAAACGGATGGACGATGTGCCGCCAGCAAAGCTCGGTATCGGTATGTTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCATaaa                                                                                                                                                                        >  1:254969/1‑251 (MQ=255)                                                    aTCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGgt                                                                                                                                                                                                                    <  1:559069/207‑1 (MQ=255)                                                    aTCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGaaa                                                                                                                                                                        >  1:710261/1‑251 (MQ=255)                                                     tCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATgg                                                                                                                                                                                                                     <  1:372775/205‑1 (MQ=255)                                                     tCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCAc                                                                                                                                                                                        >  1:649812/1‑234 (MQ=255)                                                         aCGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACAATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGAt                                                                                                                                                                                  <  1:278564/236‑1 (MQ=255)                                                            gAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTc                                                                                                                                                                 <  1:757715/250‑1 (MQ=255)                                                               aCGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCttt                                                                                                                                                              >  1:656972/1‑250 (MQ=255)                                                                cGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTc                                                                                                                                                                 <  1:25646/246‑1 (MQ=255)                                                                cGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCg                                                                                                                                                            >  1:617344/1‑251 (MQ=255)                                                                                gATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGAt                                                                                                                                                                                  <  1:281374/213‑1 (MQ=255)                                                                                 atatTAATTTGGATATTGAAGAAGGCGAATATGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCgg                                                                                                                                            <  1:667367/250‑1 (MQ=255)                                                                                   atTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGgtgt                                                                                                                                         <  1:110920/251‑1 (MQ=255)                                                                                        tttGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGtaaata                                                                                                                                    <  1:766659/251‑1 (MQ=255)                                                                                             atatTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGc                                                                                                                                                                             <  1:296222/205‑1 (MQ=255)                                                                                             atatTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGc                                                                                                                                               >  1:66429/1‑235 (MQ=255)                                                                                             atatTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAaac                                                                                                                                <  1:374348/250‑1 (MQ=255)                                                                                                 tGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAaacggaac                                                                                                                           <  1:776193/251‑1 (MQ=255)                                                                                                     gaagGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGcggtatcggtat                                                                                                                                                                                                                       >  1:724192/1‑155 (MQ=255)                                                                                                               ttGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATc                                                                                                                     <  1:374517/243‑1 (MQ=255)                                                                                                                      cTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAgg                                                                                                      <  1:520263/251‑1 (MQ=255)                                                                                                                        ttGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATgg                                                                                                                                                                                                                     <  1:723293/138‑1 (MQ=255)                                                                                                                        ttGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTAcc                                                                                                                                                                                             <  1:747416/162‑1 (MQ=255)                                                                                                                        ttGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGtt                                                                                                    >  1:629115/1‑251 (MQ=255)                                                                                                                        ttGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGtt                                                                                                    <  1:705870/251‑1 (MQ=255)                                                                                                                          gTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTg                                                                                                   >  1:90863/1‑250 (MQ=255)                                                                                                                                ccGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAAt                                                                                            >  1:756313/1‑251 (MQ=255)                                                                                                                                  gTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAAttt                                                                                          >  1:598062/1‑251 (MQ=255)                                                                                                                                  gTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAAttt                                                                                          >  1:230052/1‑251 (MQ=255)                                                                                                                                   tCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAAtttt                                                                                         <  1:616646/251‑1 (MQ=255)                                                                                                                                       gTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGaaa                                                                                                                                                    >  1:14561/1‑188 (MQ=255)                                                                                                                                            ggTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTg                                                                                 >  1:34551/1‑250 (MQ=255)                                                                                                                                                 aTCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGtaaataa                                                                                                                                   >  1:186548/1‑195 (MQ=255)                                                                                                                                                                aaTGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAAc                                                            <  1:628639/251‑1 (MQ=255)                                                                                                                                                                  tGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGcc                                                                                                                                              >  1:720629/1‑167 (MQ=255)                                                                                                                                                                           gACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTg                                                 >  1:818788/1‑251 (MQ=255)                                                                                                                                                                           gACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTg                                                 >  1:790761/1‑251 (MQ=255)                                                                                                                                                                             cTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGc                                                                                                                                                         >  1:421217/1‑145 (MQ=255)                                                                                                                                                                             cTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGtaaat                                                                                                                                     >  1:690442/1‑165 (MQ=255)                                                                                                                                                                                  ggATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCg                                          >  1:786804/1‑251 (MQ=255)                                                                                                                                                                                    atatTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGt                                        >  1:124345/1‑251 (MQ=255)                                                                                                                                                                                    atatTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGCATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGt                                        >  1:698023/1‑251 (MQ=255)                                                                                                                                                                                     tatTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGTCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTATGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACg                                                                 >  1:759593/1‑225 (MQ=255)                                                                                                                                                                                      atTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACg                                                                 >  1:289705/1‑224 (MQ=255)                                                                                                                                                                                      atTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAAc                                                            <  1:221687/229‑1 (MQ=255)                                                                                                                                                                                        tACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGcc                                                                                                                                              <  1:899153/145‑1 (MQ=255)                                                                                                                                                                                         accaccGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCg                                   >  1:846009/1‑251 (MQ=255)                                                                                                                                                                                             ccGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCaa                               >  1:171322/1‑251 (MQ=255)                                                                                                                                                                                                    aCTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTgg                        >  1:834419/1‑251 (MQ=255)                                                                                                                                                                                                       ttACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAAc                                                            <  1:351811/212‑1 (MQ=255)                                                                                                                                                                                                       ttACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTGGCGa                     >  1:788400/1‑251 (MQ=255)                                                                                                                                                                                                              ggTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTGGCGATTGGGCg              >  1:309870/1‑251 (MQ=255)                                                                                                                                                                                                               gTGAAAAACGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTGGCGATTGGGCGa             >  1:91393/1‑251 (MQ=255)                                                                                                                                                                                                                    aaaCGGATGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTGGCGATTGGGCGAAcc          >  1:231705/1‑249 (MQ=255)                                                                                                                                                                                                                          aTGAACGATGTGCCGCCGGCAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTGGCGATTGGGCGAACCCTTGTTTc  <  1:22392/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        GGTATCGAGAGGTTTCATTATGGCAAGTGTAACATTGCGCAATGTGGGCAAATCTTACGGAAACGTACATATTTCCAAAGATATTAATTTGGATATTGAAGAAGGCGAATTTGTCGTCTTTGTCGGACCGTCCGGTTGCGGTAAATCCACATTATTGCGAATGATTGCCGGACTTGAGGATATTACCACCGGTGAACTTTACATCGGTGAAAAACGGATGAACGATGTGCCGCCGGAAAAGCGCGGTATCGGTATGGTGTTCCAATCTTACGCCCTGTACCCGCACTTGGATGTGGCAGAAAATATGTCTTTCGGGCTGAAATTAGCCGGTGTAAATAAAACGGAACGGGATCAGCGCGTTAATCAGGTTGCCGAAATTTTACAGCTTGCCCATTTGCTTGAACGTAAACCGAAAGCCTTGTCGGGCGGTCAGCGTCAACGTGTGGCGATTGGGCGAACCCTTGTTTC  >  1/1135703‑1136170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑