Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,117,603	T→G	F132L (TTT→TTG)	unknown →	4‑hydroxy‑tetrahydrodipicolinate reductase (EC 1.17.1.8)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,117,603	0	T	G	100.0%	222.7 / NA	75	F132L (TTT→TTG)	unknown	4‑hydroxy‑tetrahydrodipicolinate reductase (EC 1.17.1.8)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/47);  total (28/47)

GGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGTACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTTGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGAAATTGGCTTTTCCACCATTCGCGCCTCCGATGTGG  >  1/1117358‑1117847                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gggATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGACAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCtt                                                                                                                                                                                                                                                 >  1:443927/1‑251 (MQ=255)   gATCGGCAACTTAGGCCTTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGCGTCGGCGTGAATTTGGTCTTTa                                                                                                                                                                                                                                               >  1:282920/1‑251 (MQ=255)      cGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAat                                                                                                                                                                                                                                            >  1:577589/1‑251 (MQ=255)                 gCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGaaaaa                                                                                                                                                                                                                                 >  1:105239/1‑251 (MQ=255)                           cGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAgg                                                                                                                                                                                                                       <  1:36933/251‑1 (MQ=255)                           cGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAgg                                                                                                                                                                                                                       <  1:366235/251‑1 (MQ=255)                                   aCTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATggg                                                                                                                                                                                                                >  1:285323/1‑250 (MQ=255)                                   aCTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGc                                                                                                                                                                                                               <  1:768174/251‑1 (MQ=255)                                    cTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCg                                                                                                                                                                                                              >  1:179860/1‑251 (MQ=255)                                      tAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGat                                                                                                                                                                                                            <  1:778852/251‑1 (MQ=255)                                              gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCa                                                                                                                                                                                                                          <  1:147158/229‑1 (MQ=255)                                              gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGatta                                                                                                                                                                                                          >  1:399135/1‑245 (MQ=255)                                              gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                                                                                                                                    >  1:104952/1‑251 (MQ=255)                                              gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                                                                                                                                    >  1:600100/1‑251 (MQ=255)                                              gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                                                                                                                                    >  1:602439/1‑251 (MQ=255)                                              gTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGa                                                                                                                                                                                                    >  1:158407/1‑251 (MQ=255)                                               tGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGAc                                                                                                                                                                                                   <  1:539429/251‑1 (MQ=255)                                                gAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACa                                                                                                                                                                                                  >  1:479651/1‑251 (MQ=255)                                                        gTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAat                                                                                                                                                                                           >  1:727523/1‑250 (MQ=255)                                                             gATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAatt                                                                                                                                                                                                                                           >  1:22859/1‑197 (MQ=255)                                                                     gattgattTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGa                                                                                                                                                                                                                    <  1:11784/212‑1 (MQ=255)                                                                       ttgattTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGTAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGcaccac                                                                                                                                                                           <  1:83183/251‑1 (MQ=255)                                                                         gattTCACCCGCCCGGAAGGCACATTGGAACATTTGTCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAAGTGAATCGGCAGCCGAAAAGACCGCTGTCGTGTTTGCTTCAAATTGCAGTGTCGGCGTGAATTTGGTCTGTAAATTAGTGGAAAAAGCCGGCAAgg                                                                                                                                                                                                                       <  1:872972/205‑1 (MQ=255)                                                                         gattTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGaaaa                                                                                                                                                                                                                                  >  1:401606/1‑194 (MQ=255)                                                                         gattTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAACTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCg                                                                                                                                                                         >  1:564329/1‑251 (MQ=255)                                                                          attTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGc                                                                                                                                                                        <  1:564040/251‑1 (MQ=255)                                                                               aCCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATaa                                                                                                                                                                   <  1:806613/251‑1 (MQ=255)                                                                                 ccgcccgGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAg                                                                                                                                                                 <  1:212593/251‑1 (MQ=255)                                                                                  cgcccgGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGt                                                                                                                                                                <  1:248903/251‑1 (MQ=255)                                                                                               caTTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTc                                                                                                                                                    >  1:209740/1‑250 (MQ=255)                                                                                                 ttGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGaa                                                                                                                                                                                                                                    <  1:389628/168‑1 (MQ=255)                                                                                                     aaCATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTAc                                                                                                                                              <  1:640287/250‑1 (MQ=255)                                                                                                     aaCATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTAc                                                                                                                                              <  1:449103/250‑1 (MQ=255)                                                                                                         tttGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCgcgc                                                                                                                                         <  1:117717/251‑1 (MQ=255)                                                                                                             gCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGcaccac                                                                                                                                                                           <  1:283933/213‑1 (MQ=255)                                                                                                              cATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATgcgc                                                                                                                                                        >  1:132080/1‑231 (MQ=255)                                                                                                                   tgtgTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                                                                                                                               <  1:316873/251‑1 (MQ=255)                                                                                                                   tgtgTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                                                                                                                               <  1:499402/251‑1 (MQ=255)                                                                                                                   tgtgTTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                                                                                                                               <  1:470221/251‑1 (MQ=255)                                                                                                                    gtgtTGCCAACCATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                                                                                                                               >  1:202219/1‑250 (MQ=255)                                                                                                                              ccATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGt                                                                                                                                                                <  1:250437/207‑1 (MQ=255)                                                                                                                              ccATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTAc                                                                                                                                              <  1:886353/225‑1 (MQ=255)                                                                                                                              ccATAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTg                                                                                                                    >  1:482563/1‑251 (MQ=255)                                                                                                                                 tAAAAAAATCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTTTAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCa                                                                                                                 >  1:317497/1‑251 (MQ=255)                                                                                                                                      aaaTCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAAcc                                                                                                            <  1:86861/251‑1 (MQ=255)                                                                                                                                        aTCGTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGt                                                                                                                                                                >  1:23605/1‑197 (MQ=255)                                                                                                                                           gTTATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTAc                                                                                                                                              >  1:604011/1‑212 (MQ=255)                                                                                                                                             tATCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGAc                                                                                                     <  1:681493/251‑1 (MQ=255)                                                                                                                                              aTCGGCACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACg                                                                                                    <  1:319465/251‑1 (MQ=255)                                                                                                                                                   caCCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATc                                                                                               <  1:831480/250‑1 (MQ=255)                                                                                                                                                       aCGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGaa                                                                                           <  1:249970/251‑1 (MQ=255)                                                                                                                                                          ggTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCtat                                                                                                                                                                     >  1:197615/1‑174 (MQ=255)                                                                                                                                                          ggTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATggg                                                                                                                               >  1:253910/1‑212 (MQ=255)                                                                                                                                                           gTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATc                                                                                               >  1:684463/1‑243 (MQ=255)                                                                                                                                                            ttttGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAAccc                                                                                      <  1:479383/251‑1 (MQ=255)                                                                                                                                                            ttttGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAAccc                                                                                      <  1:460923/251‑1 (MQ=255)                                                                                                                                                            ttttGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAAcc                                                                                       <  1:353079/250‑1 (MQ=255)                                                                                                                                                            ttttGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAAcc                                                                                       <  1:242244/250‑1 (MQ=255)                                                                                                                                                            ttttGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAAcc                                                                                       <  1:320091/250‑1 (MQ=255)                                                                                                                                                                 atgaCGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGc                                                                                 <  1:571202/251‑1 (MQ=255)                                                                                                                                                                            aaaGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTcg                                                                      <  1:45777/251‑1 (MQ=255)                                                                                                                                                                               gCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAAccc                                                                                      <  1:802969/232‑1 (MQ=255)                                                                                                                                                                                       tAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAGATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGtatta                                                           <  1:211376/251‑1 (MQ=255)                                                                                                                                                                                           tCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCgg                                                       <  1:646955/251‑1 (MQ=255)                                                                                                                                                                                           tCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCgg                                                       <  1:762069/251‑1 (MQ=255)                                                                                                                                                                                                  ccGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACg                                                 <  1:538494/250‑1 (MQ=255)                                                                                                                                                                                                              cTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGaa                                    <  1:311286/251‑1 (MQ=255)                                                                                                                                                                                                                    tgtTTGCTTCAAATTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGAAATTGGc                              <  1:601363/251‑1 (MQ=255)                                                                                                                                                                                                                                aTTTCAGTGTCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGtat                                                             <  1:405689/208‑1 (MQ=255)                                                                                                                                                                                                                                       tgtCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGAAATTGGCTTTTCCACCATTcgcg              <  1:364092/248‑1 (MQ=255)                                                                                                                                                                                                                                       tgtCGGCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGAAATTGGCTTTTCCACCATTCgcgc             <  1:486702/249‑1 (MQ=255)                                                                                                                                                                                                                                            gCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGAc                                                                                                     >  1:138714/1‑156 (MQ=255)                                                                                                                                                                                                                                            gCGTGAATTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGAAATTGGCTTTTCCACCATTCGCGCCTCCGAt      >  1:469416/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aaTTTGGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGAAATTGGCTTTTCCACCATTCGCGCCTCCGATgtgg  <  1:8874/250‑1 (MQ=255)                                                                                                                                                                                                                                                 aaTTTGGTCTTGAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAATCCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGTTGAAATTGGCTTTTCCACCATTCGCGCCTCCGATgtgg  <  1:879995/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GGGATCGGCAACTTAGGCATTAAGGTCGGCGATGACTTAAACGCGGTGAAAGATCGTTTCGATTTACTGATTGATTTCACCCGCCCGGAAGGCACATTGGAACATTTGGCATTTTGTGTTGCCAACCATAAAAAAATCGTTATCGGTACCACGGGTTTTGATGACGCCGGCAAAGCGGCAATTAAATCGGCAGCCGAAAAGACCGCTATCGTGTTTGCTTCAAATTTCAGTGTCGGCGTGAATTTTGTCTTTAAATTATTGGAAAAAGCCGCCAAGGTGATGGGCGATTATTGCGACATCGAAATCATCGAAGCGCACCACCGCTATAAAGTCGATGCGCCGTCCGGTACCGCGCTTTCCATGGGCGAACATATTGCCAAAACCCTCGGACGGGATCTGAAAACCCACGGCGTATTTTGTCGCGATGGTATTATCGGTGAACGTAAACGTGATGAAATTGGCTTTTCCACCATTCGCGCCTCCGATGTGG  >  1/1117358‑1117847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑