| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,113,729 | C→T | T458I (ACC→ATC) | unknown → | Glucose‑6‑phosphate isomerase (EC 5.3.1.9) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,113,729 | 0 | C | T | 99.1% | 325.1 / ‑5.0 | 112 | T458I (ACC→ATC) | unknown | Glucose‑6‑phosphate isomerase (EC 5.3.1.9) |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (57/54); total (58/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
TATCTGGGGTGAACCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACACCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGACAGTTCAAC > 1/1113484‑1113969 | tATCTGGGGTGAACCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTg > 1:466902/1‑250 (MQ=255)tATCTGGGGTGAACCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTg > 1:486675/1‑250 (MQ=255) aTCTGGGGTGAACCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGc > 1:51810/1‑250 (MQ=255) aTCTGGGGTGAACCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGc > 1:297393/1‑250 (MQ=255) gAACCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGt > 1:571171/1‑251 (MQ=255) aaCCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGt < 1:756763/250‑1 (MQ=255) aCCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTAt > 1:457547/1‑251 (MQ=255) gCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCaa > 1:87841/1‑250 (MQ=255) cACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAAc < 1:132536/250‑1 (MQ=255) ggTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGt > 1:246185/1‑236 (MQ=255) gTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAg < 1:537897/234‑1 (MQ=255) cTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCa > 1:748169/1‑251 (MQ=255) cTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCa > 1:307293/1‑251 (MQ=255) cTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCa > 1:699656/1‑251 (MQ=255) aTCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCAt > 1:719758/1‑250 (MQ=255) aTCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCAt > 1:535842/1‑250 (MQ=255) tCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCt < 1:735005/251‑1 (MQ=255) tCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCt < 1:880183/251‑1 (MQ=255) tCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCt < 1:308840/251‑1 (MQ=255) aTTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGc < 1:179435/251‑1 (MQ=255) tGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTa > 1:130546/1‑251 (MQ=255) aCCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACaaaaa > 1:842926/1‑251 (MQ=255) ccAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACaaaaaa > 1:144101/1‑251 (MQ=255) gTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCAc > 1:422340/1‑250 (MQ=255) ccACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑g < 1:742879/251‑1 (MQ=255) caTTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAGGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑Gtt > 1:453315/1‑250 (MQ=255) caTTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑Gtt > 1:247973/1‑250 (MQ=255) caTTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑Gtt > 1:214035/1‑250 (MQ=255) caTTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑Gtt > 1:371878/1‑250 (MQ=255) caTTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑Gtt > 1:365580/1‑250 (MQ=255) aTTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCt < 1:559248/251‑1 (MQ=255) aTTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCt < 1:770727/251‑1 (MQ=255) aTTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCt < 1:784757/251‑1 (MQ=255) ttCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCtt > 1:94905/1‑251 (MQ=255) cTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGt > 1:265409/1‑251 (MQ=255) tCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGcc < 1:371561/251‑1 (MQ=255) ggCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGc > 1:580352/1‑203 (MQ=255) gcgcAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATg < 1:500123/250‑1 (MQ=255) gcgcAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGa > 1:684052/1‑251 (MQ=255) cAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAac < 1:889350/250‑1 (MQ=255) cAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAac < 1:298540/250‑1 (MQ=255) cAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAac < 1:140298/250‑1 (MQ=255) cccACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCa > 1:730251/1‑165 (MQ=255) cccACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCa < 1:470399/165‑1 (MQ=255) cccACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTgaatcaattgatagaacgcgtgttga > 1:403200/1‑225 (MQ=255) cccACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACaaa > 1:517695/1‑251 (MQ=255) aaTCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAAtttt < 1:165273/251‑1 (MQ=255) aTCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAACCCCCCCCCCCCCCCCCCCCCTTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCATCCTTGGTGCCCTTATCGCCATGTATGAACACAAAAtttt > 1:798763/1‑250 (MQ=255) tCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCg > 1:366014/1‑251 (MQ=255) gTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTc > 1:480297/1‑154 (MQ=255) gTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCa > 1:133838/1‑155 (MQ=255) ttGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTAc < 1:389040/250‑1 (MQ=255) gTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTg > 1:806172/1‑212 (MQ=255) gTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAg > 1:482259/1‑251 (MQ=255) ccGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGc < 1:618295/251‑1 (MQ=255) gATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGt > 1:215904/1‑251 (MQ=255) catcaCAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTg > 1:52020/1‑249 (MQ=255) acaacaAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTc < 1:381355/251‑1 (MQ=255) acaacaAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTc < 1:33562/251‑1 (MQ=255) acaaATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCt > 1:751503/1‑165 (MQ=255) acaaATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAat > 1:215318/1‑251 (MQ=255) acaaATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAat > 1:295854/1‑251 (MQ=255) gCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAAc > 1:356721/1‑143 (MQ=255) gCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACa > 1:461825/1‑251 (MQ=255) cAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCa > 1:785789/1‑197 (MQ=255) ttcttcGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCCGGTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGAcc < 1:695662/251‑1 (MQ=255) tcGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATgg < 1:684680/251‑1 (MQ=255) cgcAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATgggg < 1:671891/250‑1 (MQ=255) agccGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTa < 1:180340/184‑1 (MQ=255) aaCCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTg < 1:571005/170‑1 (MQ=255) aaCCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGAcc < 1:365262/239‑1 (MQ=255) aaCCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATgggg < 1:665989/246‑1 (MQ=255) aCCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGa < 1:80903/251‑1 (MQ=255) aCCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGa < 1:766402/251‑1 (MQ=255) gCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGaa > 1:260517/1‑246 (MQ=255) gCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAgg > 1:238583/1‑251 (MQ=255) tAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGcc < 1:733186/250‑1 (MQ=255) aaCCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTg < 1:374993/146‑1 (MQ=255) aCCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCg < 1:136014/251‑1 (MQ=255) ccAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGc < 1:185093/69‑1 (MQ=255) gCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAAt > 1:104134/1‑165 (MQ=255) gAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCg < 1:887680/166‑1 (MQ=255) gAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAaacaac > 1:680215/1‑220 (MQ=255) aaTTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAAt < 1:554063/251‑1 (MQ=255) aTTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATa > 1:429721/1‑251 (MQ=255) ttCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATaa < 1:838250/251‑1 (MQ=255) ccTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACg > 1:356245/1‑251 (MQ=255) tGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGAt < 1:331165/251‑1 (MQ=255) cGGGCAAAACCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAAt < 1:286482/250‑1 (MQ=255) aCCTTAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCa > 1:497388/1‑251 (MQ=255) tAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGAc < 1:892456/251‑1 (MQ=255) tAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGAc < 1:252900/251‑1 (MQ=255) tAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGAc < 1:169145/251‑1 (MQ=255) tAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGAc < 1:503264/251‑1 (MQ=255) tAGCGGAAGTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGAc < 1:575425/251‑1 (MQ=255) gtataagaCATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGAc > 1:629950/5‑243 (MQ=255) gTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGc < 1:671656/101‑1 (MQ=255) gTAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGAc < 1:193306/243‑1 (MQ=255) tAGAAGACATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGACAGTTCAAc > 1:253909/1‑250 (MQ=255) gaagaCATCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAatat > 1:272034/1‑143 (MQ=255) | TATCTGGGGTGAACCGGGCACTAACGGTCAACACGCGTTCTATCAATTGATTCACCAAGGTACCACATTAATTCCTTGTGATTTCATCGCGCCGGCGCAAACCCACAATCCGTTGTCCGATCATCACAACAAATTGCTTTCCAATTTCTTCGCGCAAACCGAAGCCTTGGCTTTCGGTAAAACCAAAGAGCAAGTGGAAGCGGAATTCCTGAAAGCGGGCAAAACCTTAGCGGAAGTAGAAGACACCGTGCCGTTCAAAGTATTCAACGGCAATAAACCGACCAACTCCATCTTGCTACAAAAAATCACCCC‑GTTCAGCCTTGGTGCCCTTATCGCCATGTATGAACACAAAATTTTCGTACAAGGCGTGCTGTTCAATATTTACAGCTTCGACCAATGGGGTGTGGAATTAGGGAAACAACTCGCCAACCGCATCCTGCCGGAATTGGTGAATAACGATAAAATCGAAAGCCACGACAGTTCAAC > 1/1113484‑1113969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |