Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,101,878	T→C	N68D (AAT→GAT)	unknown ←	Aminodeoxychorismate lyase (EC 4.1.3.38)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,101,878	0	T	C	100.0%	203.6 / NA	68	N68D (AAT→GAT)	unknown	Aminodeoxychorismate lyase (EC 4.1.3.38)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/32);  total (36/32)

AGATGAGGTTGCCGATGGAACAATCGGTGACATAGCCGTTTTTGATGATCATGATTTCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATTAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACTTTTTGAATCAG  >  1/1101662‑1102120                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   aGATGAGGTTGCCGATGGAACAATCGGTGACATAGCCGTTTTTGATGATCATGATTTCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAAt                                                                                                                                                                                                                  >  1:654371/1‑251 (MQ=255)                       aTCGGTGACATAGCCGTTTTTGATGATCATGATTTCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGtt                                                                                                                                                                                            >  1:220999/1‑251 (MQ=255)                              aCATAGCCGTTTTTGATGATCATGATTTCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGttt                                                                                                                                                                                           >  1:453320/1‑245 (MQ=255)                                               gatCATGATTTCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGa                                                                                                                                                                                                  >  1:259615/1‑221 (MQ=255)                                                atCATGATTTCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGaaa                                                                                                                                                                   >  1:774816/1‑251 (MQ=255)                                                       tttCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGAc                                                                                                                                                             >  1:375908/1‑250 (MQ=255)                                                                                 aaCAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGGAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTaa                                                                                                                                  <  1:426640/251‑1 (MQ=255)                                                                                     gTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTaaa                                                                                                                                 >  1:92268/1‑248 (MQ=255)                                                                                     gTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTaaa                                                                                                                                 >  1:499460/1‑248 (MQ=255)                                                                                      tGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAAc                                                                                                                                <  1:772865/248‑1 (MQ=255)                                                                                      tGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAAc                                                                                                                                <  1:861391/248‑1 (MQ=255)                                                                                        cATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGt                                                                                                                           >  1:374946/1‑251 (MQ=255)                                                                                         aTTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAAc                                                                                                                                <  1:264392/245‑1 (MQ=255)                                                                                              aCAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCg                                                                                                                            <  1:378841/244‑1 (MQ=255)                                                                                                aaGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGa                                                                                                                                                                                                  >  1:474752/1‑172 (MQ=255)                                                                                                      cGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGttgttg                                                                                                             <  1:656915/251‑1 (MQ=255)                                                                                                           ggCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGaa                                                                                                        >  1:518215/1‑251 (MQ=255)                                                                                                            gCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGaa                                                                                                        <  1:69198/250‑1 (MQ=255)                                                                                                            gCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAg                                                                                                       >  1:713272/1‑251 (MQ=255)                                                                                                                aTTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTaa                                                                                                                                  >  1:372267/1‑220 (MQ=255)                                                                                                                aTTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTa                                                                                                   >  1:149537/1‑251 (MQ=255)                                                                                                                           tAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGt                                                                                               <  1:395746/244‑1 (MQ=255)                                                                                                                            aGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTa                                                                                         >  1:46837/1‑249 (MQ=255)                                                                                                                             gTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATg                                                                                                >  1:368379/1‑241 (MQ=255)                                                                                                                               cAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTg                                                                                    >  1:880481/1‑251 (MQ=255)                                                                                                                               cAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTg                                                                                    >  1:240275/1‑251 (MQ=255)                                                                                                                                 aTGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGc                                                                                   >  1:344693/1‑250 (MQ=255)                                                                                                                                      gTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATg                                                                                                >  1:847181/1‑232 (MQ=255)                                                                                                                                      gTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTtgtg                                                                             >  1:793031/1‑251 (MQ=255)                                                                                                                                      gTCGCAGACGATAGGCTTAAAAGTGCGGTAGG‑TTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCtt                                                                                >  1:451795/1‑247 (MQ=255)                                                                                                                                             aCGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATgg                                                                       <  1:788010/250‑1 (MQ=255)                                                                                                                                             aCGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATgg                                                                       <  1:333820/250‑1 (MQ=255)                                                                                                                                             aCGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATgg                                                                       <  1:272411/250‑1 (MQ=255)                                                                                                                                             aCGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGc                                                                      <  1:529618/251‑1 (MQ=255)                                                                                                                                              cGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGtaatcaat                                                                                                                                                                                                                                                 >  1:384962/1‑79 (MQ=255)                                                                                                                                              cGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGt                                                                                               <  1:658072/225‑1 (MQ=255)                                                                                                                                                   ggCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGt                                                                                               <  1:598146/220‑1 (MQ=255)                                                                                                                                                   ggCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGc                                                                      >  1:865888/1‑245 (MQ=255)                                                                                                                                                   ggCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGAGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATgg                                                                       >  1:146544/1‑244 (MQ=255)                                                                                                                                                    gCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCg                                                                                                                                                >  1:418679/1‑170 (MQ=255)                                                                                                                                                    gCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGt                                                                                               <  1:495701/219‑1 (MQ=255)                                                                                                                                                    gCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGtt                                                               >  1:616437/1‑251 (MQ=255)                                                                                                                                                    gCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGtt                                                               >  1:104784/1‑251 (MQ=255)                                                                                                                                                    gCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGAAAAc                                                                                                                                <  1:898962/186‑1 (MQ=255)                                                                                                                                                        aaaaGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCaa                                                           <  1:337310/251‑1 (MQ=255)                                                                                                                                                          aaGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAAt                                                         <  1:815321/251‑1 (MQ=255)                                                                                                                                                          aaGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAAt                                                         <  1:725663/251‑1 (MQ=255)                                                                                                                                                          aaGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAAt                                                         <  1:704065/251‑1 (MQ=255)                                                                                                                                                          aaGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCATACTTTGCCTTGTGCTATGGCAAGGGTTTCAAAt                                                         <  1:739375/251‑1 (MQ=255)                                                                                                                                                              gCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCaaa                                                          >  1:418456/1‑246 (MQ=255)                                                                                                                                                                           cgcgTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAAt                                        <  1:112130/251‑1 (MQ=255)                                                                                                                                                                             cgTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTa                                      <  1:96099/251‑1 (MQ=255)                                                                                                                                                                              gtgtAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTaa                                     >  1:671051/1‑251 (MQ=255)                                                                                                                                                                                   gggAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGc                                                                                                                                                  <  1:253320/137‑1 (MQ=255)                                                                                                                                                                                    ggAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGc                                                                                                                                                  <  1:742305/136‑1 (MQ=255)                                                                                                                                                                                          tAGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAAc                         <  1:410737/251‑1 (MQ=255)                                                                                                                                                                                           aGCGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCt                                                                                 >  1:879776/1‑194 (MQ=255)                                                                                                                                                                                             cGACAAAGGATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACgg                       <  1:869747/250‑1 (MQ=255)                                                                                                                                                                                                    ggATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATgg                                                                       >  1:635112/1‑195 (MQ=255)                                                                                                                                                                                                     gATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGTAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACAtata               <  1:431348/250‑1 (MQ=255)                                                                                                                                                                                                     gATTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACAtata               >  1:669065/1‑250 (MQ=255)                                                                                                                                                                                                      aTTTGTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATAc              <  1:411481/250‑1 (MQ=255)                                                                                                                                                                                                          gTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACttttt         >  1:437862/1‑251 (MQ=255)                                                                                                                                                                                                          gTTCAGCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACttttt         >  1:208745/1‑251 (MQ=255)                                                                                                                                                                                                               gCGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACTTTTTGAAt     >  1:7183/1‑250 (MQ=255)                                                                                                                                                                                                                cGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACTTTTTGAATc    <  1:629711/250‑1 (MQ=255)                                                                                                                                                                                                                cGTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACTTTTTGAATc    <  1:683621/250‑1 (MQ=255)                                                                                                                                                                                                                 gTTGTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACTTTTTGAATCAg  >  1:477479/1‑251 (MQ=255)                                                                                                                                                                                                                    gTAATCAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGTAATTCCATAATTAACCTCGTTGTAACgg                       <  1:205827/227‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   AGATGAGGTTGCCGATGGAACAATCGGTGACATAGCCGTTTTTGATGATCATGATTTCGTCACAATCGCCTTTTTGTGCGAACAGTGCATTTAACAAGGTGCGGTCGGCGTATTTTAATCCGTAGTCAATGTGGTCGCAGACGATAGGCTTAAAAGTGCGGTAGGTTTTGCGCGTGTAGGGAAAATAGCGACAAAGGATTTGTTCAGCGTTGTAATTAATTCGGCAGCGAATCAGCGGTTCGGCTCGCAATTCCGCCGGTACATGGATCTGTTTGGCAAGCTGAAAAACGTTTGGAAAAATGACCGCACTTTTGCCGTAAAACGTCTGTAAACTGCGTTCGTAGCGTTGTTGATGAAGTTTAATGTTGCGTACTTTGCCTTGTGCTATGGCAAGGGTTTCAAATAGGGGAAATTCCATAATTAACCTCGTTGTAACGGCACATATACTTTTTGAATCAG  >  1/1101662‑1102120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑