Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,085,661	T→C	Y421C (TAT→TGT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,085,661	0	T	C	100.0%	35.1 / NA	13	Y421C (TAT→TGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

GGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTTCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAATATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGATATATATAATCCAGTAATTTAATTCCTTTTTATCAGATAAAAATAATGGAGAAAGGATAGCGGGAAGAATCGCAATAAAATAACCTAT  >  1/1085426‑1085866                                                                                                                                                                                                                                            |                                                                                                                                                                                                              ggATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATg                                                                                                                                                                                                >  1:414799/1‑251 (MQ=255)                                            aaTATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTCCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGc                                                                                                                                                     <  1:459835/251‑1 (MQ=255)                                                                                              aTAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCA‑TTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGAt                                                                                                  <  1:96762/251‑1 (MQ=255)                                                                                                                            aaTCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGATATATATAAt                                                                                <  1:839763/240‑1 (MQ=255)                                                                                                                               ctctTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGATATATATAATcc                                                                              <  1:861662/239‑1 (MQ=255)                                                                                                                                                                                   aaTAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGATATATATAATCCAGTAATTTAATTCCTTTTTATCAGATAAAAATAATGGAGAAAGGATAGCGGGAAGAATCGCaat              <  1:168844/251‑1 (MQ=255)                                                                                                                                                                                         aaTGTAGGTATTGATAAAAATAAAGTGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGatata                                                                                      >  1:346212/1‑173 (MQ=255)                                                                                                                                                                                                gTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGATATATATAATCCAGTAATTTAATTCCTTTTTATCAGATAAAAATAATGGAGAAAGGATAGCGGGAAGAATCGCAATAAAATAACCtat  <  1:574356/250‑1 (MQ=255)                                                                                                                                                                                                                     tAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCt                                                                                                                                                                               >  1:680462/1‑56 (MQ=255)                                                                                                                                                                                                                     tAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGatatatat                                                                                   >  1:70900/1‑148 (MQ=255)                                                                                                                                                                                                                     tAGTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGatatat                                                                                     <  1:176805/146‑1 (MQ=255)                                                                                                                                                                                                                       gTTTTTGTTATCGGTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGATATATATAATcc                                                                              >  1:357657/1‑151 (MQ=255)                                                                                                                                                                                                                                    gTAATGAACATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGtat                                                                                                                                                <  1:892881/72‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                              GGATGTTGTAGCAATAACGGCAGCACCTAAATCAATAGGAGGTAATATAATTTTATTTTCTAAATTGTTTTTATTAAGTAATTTTTTCGTCGCATAGTAGCTATTATTATATTTTTCGTCATAAATCTCTTTGGATTCATTTTCATCGTTGAAATTTATGATTATTTTAGTTGAAATTAATAGAAATGTAGGTATTGATAAAAATAAAATGATAGTTTTTGTTATCGGTAATGAATATTTCTCTCTTAATGAGTAGCAAGTATAAGCTTGTAAAGGAATTGCAACAGACATTGCTGTATAGAACATTCTTACTTGCCAAAATATTGCCAGTAATAAATTAGTGATTAAAGCGATATATATAATCCAGTAATTTAATTCCTTTTTATCAGATAAAAATAATGGAGAAAGGATAGCGGGAAGAATCGCAATAAAATAACCTAT  >  1/1085426‑1085866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑