Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,074,705	T→C	N310S (AAT→AGT)	unknown ←	Peptide ABC transporter, substrate‑binding protein SapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,074,705	0	T	C	100.0%	203.0 / NA	69	N310S (AAT→AGT)	unknown	Peptide ABC transporter, substrate‑binding protein SapA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/29);  total (40/29)

CCTGTAAGATTCTCTTCGCCTGTTGCGGATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACATTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACATATTGGTTACGAAAGTAATTTTTCACTTTATACGGACC  >  1/1074463‑1074934                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       ccTGTAAGATTCTCTTCGCCTGTTGCGGATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                <  1:796318/251‑1 (MQ=255) ccTGTAAGATTCTCTTCGCCTGTTGCGGATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                <  1:250542/251‑1 (MQ=255)  cTGTAAGATTCTCTTCGCCTGTTGCGGATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGt                                                                                                                                                                                                                               >  1:265966/1‑251 (MQ=255)         aTTCTCTTCGCCTGTTGCGGATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACg                                                                                                                                                                                                                        <  1:280268/251‑1 (MQ=255)            ctctTCGCCTGTTGCGGATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGtt                                                                                                                                                                                                                      >  1:401770/1‑250 (MQ=255)                            gATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGTAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGtaat                                                                                                                                                                                                     >  1:291934/1‑251 (MQ=255)                            gATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                <  1:55234/224‑1 (MQ=255)                            gATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGtaat                                                                                                                                                                                                     >  1:818505/1‑251 (MQ=255)                            gATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGtaat                                                                                                                                                                                                     >  1:20707/1‑251 (MQ=255)                              ttATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGtaataa                                                                                                                                                                                                   >  1:725707/1‑251 (MQ=255)                                 taATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGcc                                                                                                                                                                                                <  1:462337/251‑1 (MQ=255)                                  aaTCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGccc                                                                                                                                                                                               <  1:560319/251‑1 (MQ=255)                                   aTCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCa                                                                                                                                                                                              >  1:246853/1‑251 (MQ=255)                                         gTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTgg                                                                                                                                                                                         >  1:834091/1‑250 (MQ=255)                                         gTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTgg                                                                                                                                                                                         >  1:659581/1‑250 (MQ=255)                                                   ggAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                >  1:681535/1‑201 (MQ=255)                                                   ggAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGtaataa                                                                                                                                                                                                                          >  1:669255/1‑207 (MQ=255)                                                      gTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATa                                                                                                                                                                           >  1:882730/1‑251 (MQ=255)                                                       tATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGAt                                                                                                                                                                            <  1:453226/249‑1 (MQ=255)                                                       tATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGAt                                                                                                                                                                            <  1:37179/249‑1 (MQ=255)                                                       tATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGAt                                                                                                                                                                            <  1:4101/249‑1 (MQ=255)                                                        aTTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                <  1:166843/196‑1 (MQ=255)                                                          tCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGAc                                                                                                                                                                       <  1:365488/251‑1 (MQ=255)                                                                        cAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGtt                                                                                                                                                                                           >  1:804411/1‑217 (MQ=255)                                                                           gAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGc                                                                                                                                                       >  1:598903/1‑250 (MQ=255)                                                                               tGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCAt                                                                                                                                                           >  1:772141/1‑242 (MQ=255)                                                                                     ggATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                >  1:151817/1‑167 (MQ=255)                                                                                          atatTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAAt                                                                                                                                       >  1:844190/1‑251 (MQ=255)                                                                                          atatTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAAt                                                                                                                                       >  1:692721/1‑251 (MQ=255)                                                                                          atatTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAAt                                                                                                                                       >  1:56170/1‑251 (MQ=255)                                                                                                 tCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCg                                                                                                                                 >  1:242989/1‑250 (MQ=255)                                                                                                         gTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACg                                                                                                                        >  1:768827/1‑251 (MQ=255)                                                                                                            gCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAt                                                                                                                      >  1:405029/1‑250 (MQ=255)                                                                                                            gCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAt                                                                                                                      >  1:384859/1‑250 (MQ=255)                                                                                                            gCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAt                                                                                                                      >  1:580801/1‑250 (MQ=255)                                                                                                             ccGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑cc                                                                                                                   <  1:750887/251‑1 (MQ=255)                                                                                                             ccGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑c                                                                                                                    <  1:149501/250‑1 (MQ=255)                                                                                                               gTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                <  1:373867/141‑1 (MQ=255)                                                                                                                   tgtgATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTgag                                                                                                             <  1:684289/251‑1 (MQ=255)                                                                                                                   tgtgATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTgag                                                                                                             <  1:734838/251‑1 (MQ=255)                                                                                                                    gtgATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTgaga                                                                                                            >  1:718951/1‑251 (MQ=255)                                                                                                                      gATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATc                                                                                                                                                                 >  1:218635/1‑197 (MQ=255)                                                                                                                      gATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGc                                                                                                                                >  1:154693/1‑230 (MQ=255)                                                                                                                             ggTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACTCTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑cc                                                                                                                   <  1:769613/235‑1 (MQ=255)                                                                                                                             ggTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGATCCCGTTGAGAGGTCaata                                                                                                    >  1:796228/1‑251 (MQ=255)                                                                                                                              gTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                >  1:542488/1‑126 (MQ=255)                                                                                                                               ttttAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCaataat                                                                                                  <  1:219228/250‑1 (MQ=255)                                                                                                                               ttttAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAatg                                                                                                 <  1:613434/251‑1 (MQ=255)                                                                                                                                  taataaTCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGAt                                                                                                                                                                            >  1:94799/1‑174 (MQ=255)                                                                                                                                   aataatCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGt                                                                                             <  1:53215/251‑1 (MQ=255)                                                                                                                                        tCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCaataa                                                                                                   >  1:787629/1‑240 (MQ=255)                                                                                                                                           gTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGa                                                                                                                       <  1:596204/218‑1 (MQ=255)                                                                                                                                           gTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑c                                                                                                                    >  1:478888/1‑220 (MQ=255)                                                                                                                                                cGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGccc                                                                                                                              >  1:606139/1‑206 (MQ=255)                                                                                                                                                                agaTAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATg                                                                                                                                                                                                                                <  1:118899/92‑1 (MQ=255)                                                                                                                                                                  aTAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑cc                                                                                                                   >  1:693712/1‑198 (MQ=255)                                                                                                                                                                          gcgTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTAc                                                                                                                         <  1:110967/185‑1 (MQ=255)                                                                                                                                                                          gcgTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAatga                                                                                                <  1:545925/209‑1 (MQ=255)                                                                                                                                                                          gcgTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTAtttt                                                      >  1:650843/1‑251 (MQ=255)                                                                                                                                                                          gcgTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTAttt                                                       <  1:518696/250‑1 (MQ=255)                                                                                                                                                                                      gTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGca                                          >  1:328275/1‑251 (MQ=255)                                                                                                                                                                                      gTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGca                                          >  1:32764/1‑251 (MQ=255)                                                                                                                                                                                               ggCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACATATTgg                                  <  1:329517/250‑1 (MQ=255)                                                                                                                                                                                               ggCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACATATTg                                   <  1:653530/249‑1 (MQ=255)                                                                                                                                                                                                   gTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACATATTGGTTACg                             >  1:173210/1‑251 (MQ=255)                                                                                                                                                                                                                          aTACGCCAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACATATTGGTTACGAAAGTAATTTTTCACTTTATAc       <  1:729472/250‑1 (MQ=255)                                                                                                                                                                                                                               ccAAATTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACATATTGGTTACGAAAGTAATTTTTCACTTTATACGGAcc  <  1:893777/250‑1 (MQ=255)                                                                                                                                                                                                                                   aTTCATGCCTTCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTgag                                                                                                             >  1:43935/1‑139 (MQ=255)                                                                                                                                                                                                                                            ttCGACACTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACatat                                     >  1:24038/1‑202 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       CCTGTAAGATTCTCTTCGCCTGTTGCGGATTATAATCATAGTCAAAATCGGGAGTATTCACCGCGGAAGCCCAAGAAATGTTCGGGATGATATTATTCGCCACGGTTGCCGTATTGTGATAAATGGTTTTAATAATCCGTTGTCGATTAATCGCTTGCGAGATAGCGCGGCGTAATTGCTCGTCTTGAAGGGCAGTTTTTTGGAAATTAAACGCCAGATACGCCAAATTCATGCCTTCGACATTTTTAATGTAATAACGTTCGTCATTTTCCCGTAATAAGCCCAGTTGGCTGACTTCGGGATAGGACGCAATCTGGCATTCGCCATTGAAAAATTTAATCAACCGCCCCGTACGAT‑CCGTTGAGAGGTCAATAATGATGTCGTTAATTTTGGCGTCTTTTTTCCAATAATCTGTATTTTTTTCTAAACGCACATATTGGTTACGAAAGTAATTTTTCACTTTATACGGACC  >  1/1074463‑1074934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑