Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,064,380 G→A intergenic (+205/‑191) unknown → / → unknown Osmotically‑inducible lipoprotein OsmB/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,064,3800GA92.3% 59.9 / ‑2.7 26intergenic (+205/‑191)unknown/unknownOsmotically‑inducible lipoprotein OsmB/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (11/13);  total (13/13)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

CGGCACTTGGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGGCGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCGTTTATTTATATTATTTATAATAGATTAATTAAGTCAGCTATTGAG  >  1/1064135‑1064559
                                                                                                                                                                                                                                                     |                                                                                                                                                                                   
cGGCACTTGGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGacgcgc                                                                                                                                                                                <  1:169811/251‑1 (MQ=255)
          cGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAggg                                                                                                                                                                      <  1:77441/251‑1 (MQ=255)
          cGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCA‑TTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAggg                                                                                                                                                                      <  1:554485/250‑1 (MQ=255)
                  ttGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAg                                                                                                                                                              <  1:107148/251‑1 (MQ=255)
                     gAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAggg                                                                                                                                                                      >  1:806376/1‑240 (MQ=255)
                     gAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGCGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACa                                                                                                                                                           >  1:532233/1‑251 (MQ=255)
                                   aaaTAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTtat                                                                                                                                                                >  1:867558/1‑232 (MQ=255)
                                   aaaTAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGtttttt                                                                                                                                             >  1:708186/1‑251 (MQ=255)
                                            atttTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGAc                                                                                                                                                            >  1:797658/1‑227 (MQ=255)
                                            atttTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCA‑TTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTaa                                                                                                                                    >  1:68217/1‑250 (MQ=255)
                                                            aaaGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACaa                                                                                                                     <  1:746276/250‑1 (MQ=255)
                                                             aaGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAAc                                                                                                                   <  1:896755/251‑1 (MQ=255)
                                                                ccAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACtg                                                                                                                 <  1:314764/250‑1 (MQ=255)
                                                                 cAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACtgtg                                                                                                               <  1:476513/251‑1 (MQ=255)
                                                                             cAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACaa                                                                                                                     >  1:550647/1‑233 (MQ=255)
                                                                                aaaTTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGAt                                                                                                <  1:43455/251‑1 (MQ=255)
                                                                                aaaTTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGAt                                                                                                <  1:30888/251‑1 (MQ=255)
                                                                                                      gCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAaatg                                                                                    <  1:793014/241‑2 (MQ=255)
                                                                                                       cATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGAt                                                                                                >  1:205842/1‑228 (MQ=255)
                                                                                                           tttttGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATGATTTTAAGATTGaa                                                                      >  1:803828/1‑250 (MQ=255)
                                                                                                                                 aTCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATGATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCg                                               >  1:306897/1‑251 (MQ=255)
                                                                                                                                  tctcAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTtat                                                                                                                                                                <  1:716553/137‑1 (MQ=255)
                                                                                                                                  tctcAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATGATTTTAAGAt                                                                          <  1:764852/223‑1 (MQ=255)
                                                                                                                                                            gTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAg                                                                                                                                                              >  1:379124/1‑113 (MQ=255)
                                                                                                                                                                              aaaaaGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGACGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATGATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCGTTTATTTATATTATTTATAATAGATTAATTAAGTCAGCTATTGAg  <  1:10422/251‑1 (MQ=11)
                                                                                                                                                                                                                 ggTAAGGATAAAAGCCTATAATACGCGGGCACAACGGCGCGCCGTTGTAGGGTTTTATAGAc                                                                                                                                                            >  1:462608/1‑62 (MQ=37)
                                                                                                                                                                                                                              gCCTATAATACGCGGGCACAACGGCGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAAc                                                                                                                   >  1:147215/1‑90 (MQ=35)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                   
CGGCACTTGGCGGTTTGATTGGAAGCCAAGTGGGCAAATAATTTATTTTACTTAACTCGGAAAGCCAAAAGTGCGGTCAGAAATTCAAGCGTTTTTCTGACCGCATTTTTTTGATCTCCATAATTTTTTATCTCAAAGGAAAACACAATTTGGCGAGTTTATGAGCAAATAATAAAAAAGTTCAATTTTTTGTAAAAAAGTACTTGCAAGGTAAGGATAAAAGCCTATAATACGCGGGCACAACGGCGCGCCGTTGTAGGGTTTTATAGACAGCGCGTCGTTTTTTGTTCTTTAACAATCAATCATACAAACTGTGTGGGCACTTATAGATTCGTTTTAAATAATTTTAAGATTGAACTTAATAGGTGCCGGAAGATTCGTTTATTTATATTATTTATAATAGATTAATTAAGTCAGCTATTGAG  >  1/1064135‑1064559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑