| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,056,712 | A→G | S416P (TCG→CCG) | unknown ← | Phosphoglucosamine mutase (EC 5.4.2.10) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,056,712 | 0 | A | G | 98.8% | 211.7 / NA | 81 | S416P (TCG→CCG) | unknown | Phosphoglucosamine mutase (EC 5.4.2.10) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (44/36); minor base T (0/1); total (44/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCAAGTCAAGTGCGGTGGATTTTAACCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGATTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATGCCATCCCCGGTAGTGTTG > 1/1056470‑1056953 | aTCCAAGTCAAGTGCGGTGGATTTTAACCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTa > 1:653302/1‑250 (MQ=255)aTCCAAGTCAAGTGCGGTGGATTTTAACCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTAc < 1:17040/251‑1 (MQ=255)aTCCAAGTCAAGTGCGGTGGATTTTAACCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTAc < 1:562840/251‑1 (MQ=255) tGCGGTGGATTTTAACCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGc < 1:396658/250‑1 (MQ=255) aaCCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGccc < 1:504814/251‑1 (MQ=255) aCCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGcc > 1:685240/1‑249 (MQ=255) cAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑ttt > 1:89689/1‑251 (MQ=255) gTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGc < 1:593982/231‑1 (MQ=255) tCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCg > 1:611128/1‑251 (MQ=255) ggATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGc < 1:725223/217‑1 (MQ=255) gTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCAt > 1:502039/1‑250 (MQ=255) tCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCAtct > 1:116959/1‑250 (MQ=255) tCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCAtct > 1:77314/1‑250 (MQ=255) tCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCAtct > 1:144661/1‑250 (MQ=255) cATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCAtt > 1:25028/1‑251 (MQ=255) cATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCAtt > 1:331045/1‑251 (MQ=255) cATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTCTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCAtt > 1:755962/1‑251 (MQ=255) aCGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCAc < 1:108013/249‑1 (MQ=255) ggCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑cc > 1:479645/1‑224 (MQ=255) ggCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGca > 1:728406/1‑251 (MQ=255) ttCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGcaccac < 1:111622/251‑1 (MQ=255) ttCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGcaccac < 1:136554/251‑1 (MQ=255) ccTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCAc < 1:644657/241‑1 (MQ=255) ccTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACtt > 1:795530/1‑251 (MQ=255) tATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTcc < 1:642714/251‑1 (MQ=255) ccATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTca > 1:270988/1‑251 (MQ=255) aTTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAAcc < 1:313653/212‑1 (MQ=255) ttCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGAttt > 1:460094/1‑156 (MQ=255) tCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACg < 1:529764/251‑1 (MQ=255) ccGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGA‑TTTCACCACCGGCAAAACGTACa > 1:303150/1‑251 (MQ=255) tGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACAt < 1:499736/250‑1 (MQ=255) cATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGAtt < 1:845474/250‑1 (MQ=255) cATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGAtt < 1:498073/250‑1 (MQ=255) tAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAg > 1:324011/1‑250 (MQ=255) tAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAg > 1:65795/1‑250 (MQ=255) aaCAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCG‑TTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCAc < 1:722298/251‑1 (MQ=255) aCAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCa > 1:737581/1‑231 (MQ=255) aCAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCAc < 1:801550/251‑1 (MQ=255) cAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTAc > 1:250318/1‑126 (MQ=255) cAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCaaaa > 1:447744/1‑233 (MQ=255) aCGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTcac < 1:63923/215‑1 (MQ=255) aCGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGa > 1:310115/1‑251 (MQ=255) cGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTc < 1:468330/186‑1 (MQ=255) gCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGaat > 1:810112/1‑251 (MQ=255) ccATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAAc > 1:775960/1‑172 (MQ=255) ccATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTg < 1:499608/251‑1 (MQ=255) ccATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTg > 1:666429/1‑251 (MQ=255) ccATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTg < 1:34442/251‑1 (MQ=255) aTTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGa > 1:461582/1‑250 (MQ=255) tCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCAc > 1:692563/1‑250 (MQ=255) tcttTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCAtt > 1:297857/1‑251 (MQ=255) cttTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTAc < 1:249729/90‑1 (MQ=255) cttTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACAt < 1:608250/204‑1 (MQ=255) cttTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACAt < 1:390892/204‑1 (MQ=255) tgctgcATTTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATCCCCCCATGAGGCGGATAAGCGGTTGGGTCCCTGTTTTACGCAACAAAATTCGCCT‑TTTGCCCGCCACCCGTTTTC‑CCATCTCGGCAGCACCCATTTTCCCCGCCCCTTTTTCCAACGGTTTTTCCCCACCGGCAAAACGTACATTGATTAGCACTTGCGGAAATATTTTGCCCGCACCCGCCAATTCATTAAGGGACAAAc < 1:323698/251‑1 (MQ=255) tgctgcAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACaaa > 1:255271/1‑250 (MQ=255) ctgcATTTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACTTCCCCCCATGAGGCGGATAAGCGGTTCGTTCCTTGTTTTACGAAAAAAAATTCCCCC‑TTTCCCCGCCAACCTTTTTC‑CCATCTCGGCAGCACCCATTTCCCCCGCCCCCTTTTCCAACGGTTTTCCCCCCCCGGCAAAACGTACATTGATTAGCCCTTGCGGGAATAATTTGCCCGCCCCCGCCAATTCATTAAGGGACAAACGa < 1:601241/251‑1 (MQ=255) cttctGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑cc > 1:536893/1‑116 (MQ=255) gCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTCCCTGATTTACGAAAAAAAATTCGCCC‑TTTGCCCCCCACCCGTTTTT‑CCATCTCGGCAGCAACCATTTTCCCCGCCCCCCTTTCCAACGGTTTTTCCCCCCCGGCAAAACGTACATTGATTAGCACTGGCGGGAATAATTTGCCCGCCCCCGCCAATTCATTAAGGGACAAACGATGTTTTGc < 1:502087/245‑1 (MQ=255) gCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACTAACg > 1:495404/1‑236 (MQ=255) gCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCg > 1:528423/1‑250 (MQ=255) gtgCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTaa > 1:680560/1‑216 (MQ=255) gtgCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACg > 1:265613/1‑251 (MQ=255) tgcaaaaGTGCATTCTTCCCATCCCACCATGATGCGGATAGGGGGTTCGGTACCTGATTTACGCAAAAAAATTCCCCC‑TTTGCCCGCCACCCTTTTTC‑CCATCTCGGCAGCACCCATTTCCCCCGCCCCCCTTTCCAACGGATTTTCCCCCCCGGAAAAACGTACATTGATTAGCACTGGCGGAAATATTTTGCCCCCCCCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACgg < 1:695254/247‑1 (MQ=255) gCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCAc < 1:752344/176‑1 (MQ=255) aGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAagag > 1:144901/1‑251 (MQ=255) gTGCCATCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGcc > 1:394737/1‑237 (MQ=255) tCTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCaat > 1:822806/1‑251 (MQ=255) cTTCACATTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCaata > 1:153358/1‑251 (MQ=255) cacaTCCCCCCAGGATGCGGAAAAGCGGTTCGTTACCTGATCCCCGCAACAAAATTCGCCC‑TTTCCCCCCCACCCGTTTTC‑CCATCCCGGCAGCAACCATTTCCCCCCCCCCACTTTCCAACGGATTTCCCCCCCCGGAAAAACGTACATTGATTAGCACTTGGGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATg < 1:848077/251‑1 (MQ=255) ccatccccccaTGATGCGAATAGGCGTTCCGGTCCCTGATTTACCCAACAAAATCCCCCC‑TTTCCCCCCCACCCTTTTTC‑CCATCCCGGCACCAACCATTTCCCCCGCCCCCCTTCCCAACGAATTTCCCCCCCCGCAAAAACGTACATTGATTAGCACTTGGGGAAATATTTTGACCGCCCCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATGc < 1:386430/243‑1 (MQ=255) acaTTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTc > 1:67853/1‑192 (MQ=255) acaTTCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATg > 1:362872/1‑250 (MQ=255) cacccccccaTGAGGCGGATAAGCGGTTCGGTCCCTGATTTCCGCAAAAAAATCCCCCC‑TCTCCCCCCCACCCGTTTTC‑CCTTCCCGGCAGCACCCTTTTTCCCCGCCCCTTTTTCCAACGGTTTTCCCCCCCCGGAAAAACGTACATTGATTAGCCTTTGCGGGAATATTTGGCCCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATAc < 1:444897/223‑1 (MQ=255) ttCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGc > 1:727898/1‑231 (MQ=255) ttCCACCATGATGCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAagag > 1:620410/1‑236 (MQ=255) cccccaTGATGCGGATAAGGGGTTGGGTCCCTGATTTACGCAACAAAATTCCCCC‑TTT‑CCCCCCAACCCTTTTTCCCATCGCGGCAGCAACCATTTCCCCCGCACCATTTCCCAACGGATTTCCCCCCCCGACAAACCGTACATTGATTAGCACTTGCGGAAATAATTTGCCCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATGCCATcc < 1:881159/248‑1 (MQ=255) atgatgCGGATAAGCGGTTCGTTCCCTGTTTTCCCCAACAAAATCCGCCC‑TTTGCCCCCCACCCTTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCCCCACTTTCCAACGGTTTTTCCCCCCCGGCAAAACGTACATTGATTAGCACTTGGGGGAATAATTGGCCCGCCCCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATGCCATCCCCGGt < 1:696005/251‑1 (MQ=255) gatgCGGATAAGCGGTTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCAc > 1:391630/1‑93 (MQ=255) gggATAAGGGGTTCGGTCCCGGATTTACGAAAAAAAATCCGCCC‑TTTCCCCGCCACCCTTTTTC‑CCATCTCGGCAGCACCCATTTTCACCGCCCCACTTTCCAACGGATTTTCCCCCCCGGAAAAACGTACATTGATTAGCACTTGCGGAAATAATTTGCCCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATGCCATCCCCGGTAgtgt < 1:263714/249‑1 (MQ=255) gATAAGCGGTCCGGTACCTGTTTTACGCAAC‑AAATTCCCCCTTTTCCCCGCAAACCTTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCTCTTCCCAACGGATTTTCCCCCCCGGCAAAACGTACATTGATTAGCACTTGCGGAAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATGCCATCCCCGGTAGTGTTg < 1:378110/250‑1 (MQ=255) | ATCCAAGTCAAGTGCGGTGGATTTTAACCAAGTTCCTTGCAAAGTGGATTGTTCCTTACCGCGCGCATTAAGACGGCGTTCCTTATCAGAATTCACCATTAATTCCGCTTCGCCGTGACGCATAACAAATAAACGCATGACCATTAAATCCTAGTTGGCTTCTTTTACTGCTGCTGCAATTTCTTCTGCACATTGCTGTGCCAAAGTGCCATCTTCACATTCCACCATGATGCGGATAAGCGATTCGGTACCTGATTTACGCAACAAAATTCGCCC‑TTTGCCCGCCAACCGTTTTT‑CCATCTCGGCAGCAACCATTTTCACCGCACCACTTTCCAACGGATTTTCACCACCGGCAAAACGTACATTGATTAGCACTTGCGGGAATAATTTGACCGCACCCGCCAATTCATTAAGGGACAAACGATGTTTTGCCATCGCAGCCAATACGGCAAGAGAAGCAATAATGCCATCCCCGGTAGTGTTG > 1/1056470‑1056953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |