Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,052,002	T→C	V167A (GTA→GCA)	unknown →	Peptide chain release factor N(5)‑glutamine methyltransferase (EC 2.1.1.297)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,052,002	0	T	C	98.5%	193.6 / NA	68	V167A (GTA→GCA)	unknown	Peptide chain release factor N(5)‑glutamine methyltransferase (EC 2.1.1.297)
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (38/29);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTTCGGCAGGCACGCTGATTCCACGCCCCGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGTATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCATATTATCGCCAATGCGCCGAAATTT  >  1/1051762‑1052237                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            tttCGGCAGGCACGCTGATTCCACGCCCCGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGaaa                                                                                                                                                                                                                                   >  1:889373/1‑251 (MQ=255)    cGGCAGGCACGCTGATTCCACGCCCCGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCt                                                                                                                                                                                                                                <  1:732018/251‑1 (MQ=255)          gcaCGCTGATTCCACGCCCCGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACgg                                                                                                                                                                                                                           <  1:514524/250‑1 (MQ=255)               cTGATTCCACGCCCCGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGaaaa                                                                                                                                                                                                                     >  1:728278/1‑251 (MQ=255)                 gATTCCACGCCCCGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGaaaaaa                                                                                                                                                                                                                   >  1:134548/1‑251 (MQ=255)                          ccccGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGa                                                                                                                                                                                                          >  1:558971/1‑251 (MQ=255)                                    gAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTg                                                                                                                                                                                                           >  1:723313/1‑240 (MQ=255)                                         atTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGc                                                                                                                                                                                           <  1:818400/251‑1 (MQ=255)                                          ttGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCa                                                                                                                                                                                          >  1:286502/1‑251 (MQ=255)                                                      gCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGtt                                                                                                                                                                              >  1:681247/1‑251 (MQ=255)                                                      gCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGt                                                                                                                                                                               <  1:330357/250‑1 (MQ=255)                                                                     aTTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACggg                                                                                                                                                               <  1:321385/251‑1 (MQ=255)                                                                             gCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTg                                                                                                                                                       >  1:898618/1‑251 (MQ=255)                                                                              cTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACAttt                                                                                                                                                        <  1:567172/249‑1 (MQ=255)                                                                                aCAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGAtt                                                                                                                                                    >  1:798677/1‑251 (MQ=255)                                                                                       tGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATcc                                                                                                                                              <  1:590020/250‑1 (MQ=255)                                                                                       tGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATcc                                                                                                                                              <  1:569226/250‑1 (MQ=255)                                                                                           gTCAAAATTCGCAGCG‑TTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGt                                                                                                                                         <  1:778000/250‑1 (MQ=255)                                                                                            tCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGt                                                                                                                                         <  1:861192/250‑1 (MQ=255)                                                                                            tCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGt                                                                                                                                         <  1:548764/250‑1 (MQ=255)                                                                                                   tCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACccgc                                                                                                                                  >  1:184844/1‑250 (MQ=255)                                                                                                         cGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTatat                                                                                                                           <  1:753445/251‑1 (MQ=255)                                                                                                         cGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTatat                                                                                                                           <  1:500196/251‑1 (MQ=255)                                                                                                           ttttCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAACCCTATTGCACAAAAACACCCGGTTAAGTTGGATATTATGGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTTTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTg                                                                                                                         <  1:416207/251‑1 (MQ=255)                                                                                                            tttCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAACCCTATTGCACAAAAACACACGGTTCAGTGGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGa                                                                                                                        <  1:93/251‑1 (MQ=255)                                                                                                            tttCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGa                                                                                                                        <  1:540340/251‑1 (MQ=255)                                                                                                            tttCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGa                                                                                                                        <  1:509362/251‑1 (MQ=255)                                                                                                                 cATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTAACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTACTCCTGAGTAACCCGCCGTATATTGATGccc                                                                                                                   <  1:783530/251‑1 (MQ=255)                                                                                                                                   cggcaccggcGCGATTGCTTTGGCATTGGCTTCGGCGTTAAACCCTATTGCACAAAAACACACGGTTCGGTTGGATATTATTGGCTTTGACCTCACGCCTGGGGTGGTGGCTTTGGCGAAATCTAACGGGGAAAAAAATCAATTAAATGTGCCTTTTGTACAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCTTGGGAAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGc                                                                                                 <  1:565186/251‑1 (MQ=255)                                                                                                                                     gcaccggcGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAg                                                                                               >  1:782867/1‑251 (MQ=255)                                                                                                                                     gcaccggcGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAg                                                                                               >  1:104897/1‑251 (MQ=255)                                                                                                                                     gcaccggcGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAg                                                                                               >  1:231549/1‑251 (MQ=255)                                                                                                                                     gcaccggcGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAg                                                                                               >  1:32749/1‑251 (MQ=255)                                                                                                                                        ccggcGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCCCAAAACCACACGGTTCAGTTGAATATTATTGCCTTTGACCTCACGCTTGAGGTGTTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGTTTTTAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAGCCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGt                                                                                            <  1:388342/251‑1 (MQ=255)                                                                                                                                           gcgcGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGAt                                                                                         >  1:139827/1‑251 (MQ=255)                                                                                                                                              cgATTGCTTTGGCATTGGCTTCGGCGTTAAACCCTATTGCACAAAAACGCACGGTTCAGTTGGATATTATTGGTGTTGACCTCACGCCTGAGGTGGTGGTATTGGCGAAATCTAACGGGAAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTGGTTGGTTTGAAAATGTTACGGGGAAATTGAATTTAATCCTGGGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGAtgtg                                                                                      <  1:209777/251‑1 (MQ=255)                                                                                                                                                   gCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGt                                                                                                                                         <  1:393054/195‑1 (MQ=255)                                                                                                                                                              ggCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTatat                                                                                                                           >  1:552619/1‑198 (MQ=255)                                                                                                                                                                     gCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCAtt                                                               >  1:875579/1‑251 (MQ=255)                                                                                                                                                                     gCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCAtt                                                               >  1:467324/1‑251 (MQ=255)                                                                                                                                                                      cGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCAtt                                                               <  1:512245/250‑1 (MQ=255)                                                                                                                                                                            aaCCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACGTTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCa                                                        >  1:142345/1‑251 (MQ=255)                                                                                                                                                                                    gCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACa                                                                                                         >  1:504348/1‑194 (MQ=255)                                                                                                                                                                                     cacaAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGgaagaa                                               >  1:100389/1‑251 (MQ=255)                                                                                                                                                                                                 cGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGa                                                                                                                        <  1:117139/166‑1 (MQ=255)                                                                                                                                                                                                   gTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTatat                                                                                                                           >  1:125648/1‑161 (MQ=255)                                                                                                                                                                                                   gTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTatat                                                                                                                           <  1:487623/161‑1 (MQ=255)                                                                                                                                                                                                   gTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGAttt                                 >  1:573708/1‑251 (MQ=255)                                                                                                                                                                                                   gTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGAttt                                 >  1:638304/1‑251 (MQ=255)                                                                                                                                                                                                   gTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGAttt                                 >  1:236725/1‑251 (MQ=255)                                                                                                                                                                                                        gTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGa                                                                                                                        <  1:210832/159‑1 (MQ=255)                                                                                                                                                                                                         ttGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGc                             <  1:22633/249‑1 (MQ=255)                                                                                                                                                                                                              tattatTGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGAtt                                                                                                                                                    <  1:757692/125‑1 (MQ=255)                                                                                                                                                                                                                ttattGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTatat                                                                                                                           >  1:2406/1‑148 (MQ=255)                                                                                                                                                                                                                ttattGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGc                                                                                     >  1:663897/1‑186 (MQ=255)                                                                                                                                                                                                                ttattGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGc                                                                  >  1:37957/1‑205 (MQ=255)                                                                                                                                                                                                                ttattGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCAtattat                     >  1:550294/1‑250 (MQ=255)                                                                                                                                                                                                                ttattGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCAtattat                     >  1:292669/1‑250 (MQ=255)                                                                                                                                                                                                                ttattGGCGTTGACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCAtattat                     >  1:518368/1‑250 (MQ=255)                                                                                                                                                                                                                     ggagTTGTCCTCACGCGTGAGGTGGTGGCATTGGCGAAATCTAGCGGGGAAAAAAAGCAATGGATTGTGGCTTTTGTGCAAATTTGTTGGTTTGAAATGGTTACGGGGAAATTTGATTTAATCCAGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGc                                                                                                 <  1:703335/166‑1 (MQ=255)                                                                                                                                                                                                                           gACCTCACGCCTGAGGTGGTGGCATTGTCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCAt                                                                >  1:80362/1‑196 (MQ=255)                                                                                                                                                                                                                           gACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGttgttg                                                                                                                                                                                 <  1:523579/83‑1 (MQ=255)                                                                                                                                                                                                                           gACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGAtg                                                                                        >  1:559139/1‑172 (MQ=255)                                                                                                                                                                                                                           gACCTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCATATTATCGCCAATgcgc          >  1:623092/1‑250 (MQ=255)                                                                                                                                                                                                                             ccTCACGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCATATTATCGCCAATGCGCCGa       >  1:224563/1‑251 (MQ=255)                                                                                                                                                                                                                                  cGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATcc                                                                                                                                              >  1:138044/1‑111 (MQ=255)                                                                                                                                                                                                                                  cGCCTGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCATATTATCGCCAATGCGCCGAAAttt  >  1:414791/1‑251 (MQ=255)                                                                                                                                                                                                                                      tGAGGTGGTGGCATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGc                                                                                                 >  1:630232/1‑152 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            TTTCGGCAGGCACGCTGATTCCACGCCCCGACACGGAAATATTGGTGGAGAAGGCAGTGGCGATTGCCATTGAAAAGCTACAAAAGTGCGGTCAAAATTCGCAGCGTTTTCGCATTCTCGATCTTGGCACCGGCACCGGCGCGATTGCTTTGGCATTGGCTTCGGCGTTAAAACCTATTGCACAAAAACACACGGTTCAGTTGGATATTATTGGCGTTGACCTCACGCCTGAGGTGGTGGTATTGGCGAAATCTAACGGGGAAAAAAATCAATTGAATGTGGCTTTTGTGCAAAGTTGTTGGTTTGAAAATGTTACGGGGACATTTGATTTAATCCTGAGTAACCCGCCGTATATTGATGCCCATGACGAACATTTAACGCAGGGTGATGTGCGTTTTGAACCCTTGTCCGCATTGGTTGCAGCGGAAGAAGGTTACGCGGATTTACGCCATATTATCGCCAATGCGCCGAAATTT  >  1/1051762‑1052237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑