Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,050,743	T→C	G121G (GGT→GGC)	unknown →	Peptide chain release factor 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,050,743	0	T	C	100.0%	213.4 / NA	72	G121G (GGT→GGC)	unknown	Peptide chain release factor 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/36);  total (36/36)

GAATATGCACAACTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGTGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGCCTGTACTG  >  1/1050501‑1050987                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     gAATATGCACAACTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAgg                                                                                                                                                                                                                                               >  1:6331/1‑250 (MQ=255) gAATATGCACAACTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAgg                                                                                                                                                                                                                                               >  1:289446/1‑250 (MQ=255)  aaTATGCACAACTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAgg                                                                                                                                                                                                                                               <  1:239250/249‑1 (MQ=255)            aCTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCg                                                                                                                                                                                                                                   >  1:44117/1‑251 (MQ=255)             cTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGcgatg                                                                                                                                                                                                                                                  >  1:357689/1‑235 (MQ=255)             cTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCg                                                                                                                                                                                                                                   >  1:887927/1‑250 (MQ=255)                     tgttgtGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGAtt                                                                                                                                                                                                                          <  1:214310/251‑1 (MQ=255)                          tgAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGGTGAGGCGGGCATTTTCGCCGGTGATTTATTc                                                                                                                                                                                                                     <  1:813454/251‑1 (MQ=255)                          tgAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTc                                                                                                                                                                                                                     <  1:308924/251‑1 (MQ=255)                                   ttAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTAc                                                                                                                                                                                                            <  1:458021/251‑1 (MQ=255)                                              aaTCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATg                                                                                                                                                                                                  >  1:473601/1‑250 (MQ=255)                                              aaTCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATg                                                                                                                                                                                                  <  1:83208/250‑1 (MQ=255)                                              aaTCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATg                                                                                                                                                                                                  >  1:796959/1‑250 (MQ=255)                                                   gCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGaaa                                                                                                                                                                                            >  1:205947/1‑251 (MQ=255)                                                      cAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCa                                                                                                                                                                                         >  1:749966/1‑251 (MQ=255)                                                        aTTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGc                                                                                                                                                                                                         <  1:233196/233‑1 (MQ=255)                                                        aTTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCgg                                                                                                                                                                                               <  1:425559/243‑1 (MQ=255)                                                        aTTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCaaa                                                                                                                                                                                       >  1:175285/1‑251 (MQ=255)                                                            cAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGtt                                                                                                                                                                                   <  1:653735/251‑1 (MQ=255)                                                             aaTCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGGTTTATTCCGCATGTACAGc                                                                                                                                                                                                         >  1:341626/1‑228 (MQ=255)                                                               tCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTc                                                                                                                                                                                                                     <  1:755601/214‑1 (MQ=255)                                                                  ggAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCgtg                                                                                                                                                                             >  1:464982/1‑251 (MQ=255)                                                                         gcgcAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAgg                                                                                                                                                                      >  1:826574/1‑250 (MQ=255)                                                                          cgcAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAg                                                                                                                                                                       <  1:239993/249‑1 (MQ=255)                                                                             aGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCt                                                                                                                                                                   >  1:314077/1‑250 (MQ=255)                                                                              gCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCa                                                                                                                                                                 >  1:295754/1‑251 (MQ=255)                                                                              gCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCa                                                                                                                                                                 >  1:763725/1‑251 (MQ=255)                                                                                 tttGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTg                                                                                                                                                              <  1:725390/251‑1 (MQ=255)                                                                                  ttGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGa                                                                                                                                                             <  1:632920/251‑1 (MQ=255)                                                                                            aTCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTc                                                                                                                                                                                                                     >  1:836285/1‑185 (MQ=255)                                                                                              cccGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGa                                                                                                                                                 >  1:202567/1‑251 (MQ=255)                                                                                                                                                     aaCAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATgg                                                                                           <  1:799946/250‑1 (MQ=255)                                                                                                                                                     aaCAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGt                                                                                          <  1:247459/251‑1 (MQ=255)                                                                                                                                                         ggTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGGCAGt                                                                                      >  1:810001/1‑251 (MQ=255)                                                                                                                                                          gTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTc                                                                                         <  1:643700/247‑1 (MQ=255)                                                                                                                                                              aaCAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTaaaa                                                                                 <  1:565834/251‑1 (MQ=255)                                                                                                                                                                  gCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTAt                                                                                             <  1:102046/235‑1 (MQ=255)                                                                                                                                                                  gCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATgg                                                                                           >  1:56589/1‑237 (MQ=255)                                                                                                                                                                  gCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGtgtctcttataaa                                                                              >  1:384718/1‑238 (MQ=255)                                                                                                                                                                  gCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTg                                                                             >  1:222873/1‑251 (MQ=255)                                                                                                                                                                  gCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGGGATTTATTCCGTATGTACAGCCGTTATGGGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAg                                                                                                                                >  1:612795/1‑200 (MQ=255)                                                                                                                                                                      ttACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAGAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGGATGGTCAGTTTAAATTTGAAt                                                                          >  1:648628/1‑250 (MQ=255)                                                                                                                                                                        aCAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAg                                                                                                                                <  1:84839/194‑1 (MQ=255)                                                                                                                                                                          aaaTTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGGTAAAATTTGAATCgggg                                                                     >  1:318797/1‑250 (MQ=255)                                                                                                                                                                                        ccGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGc                                                        <  1:20210/250‑1 (MQ=255)                                                                                                                                                                                        ccGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGc                                                        <  1:462809/250‑1 (MQ=255)                                                                                                                                                                                                       gacgaATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAAtt                                                                               <  1:248498/212‑1 (MQ=255)                                                                                                                                                                                                          gaATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGc                                                                                                                                                                                                         <  1:150018/87‑1 (MQ=255)                                                                                                                                                                                                          gaATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGt                                                                                          >  1:499355/1‑198 (MQ=255)                                                                                                                                                                                                          gaATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATc                                                                         >  1:239592/1‑215 (MQ=255)                                                                                                                                                                                                          gaATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGc                                               <  1:156882/241‑1 (MQ=255)                                                                                                                                                                                                          gaATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGGAAACTGa                                     >  1:342653/1‑251 (MQ=255)                                                                                                                                                                                                            atatAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAAt                                   >  1:62721/1‑251 (MQ=255)                                                                                                                                                                                                            atatAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGGATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAAt                                   >  1:736386/1‑251 (MQ=255)                                                                                                                                                                                                                  cTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGt                                                                                          <  1:97001/190‑1 (MQ=255)                                                                                                                                                                                                                  cTGTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAg                             <  1:412270/251‑1 (MQ=255)                                                                                                                                                                                                                    gTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCCAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAggg                           >  1:891495/1‑251 (MQ=255)                                                                                                                                                                                                                    gTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTa                                                                                    >  1:475048/1‑194 (MQ=255)                                                                                                                                                                                                                    gTTATCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAggg                           >  1:275790/1‑251 (MQ=255)                                                                                                                                                                                                                       aTCTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTTATTTATTCCGTATGTACAGCCGTTATTCGGAAACCAAACGTTGGCGTGTTTTAGTGCTCAGGGAAAATGAAAGCGAGCAAGGCGGCTTTAAAGAAATTATCGTGTTGGTCAGCGGTGACGGGGTGTATGGTTAGTTAAAATTTGAATc                                                                         >  1:604505/1‑202 (MQ=255)                                                                                                                                                                                                                         cTTGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTAt                      >  1:527938/1‑251 (MQ=255)                                                                                                                                                                                                                           tGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTc                    <  1:882073/251‑1 (MQ=255)                                                                                                                                                                                                                           tGAAATCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTc                    <  1:842845/251‑1 (MQ=255)                                                                                                                                                                                                                               aTCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCacac                <  1:179593/251‑1 (MQ=255)                                                                                                                                                                                                                                tCCGCGCCGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACAcc               <  1:566488/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccggtgccggtgGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGcc         <  1:534222/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccggtgccggtgGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGcc         <  1:750673/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGcc         <  1:544599/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccGGTACCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGGGCAGCGAGGGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGc          >  1:729423/1‑250 (MQ=255)                                                                                                                                                                                                                                           aCCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGCCTGTAc    <  1:797690/251‑1 (MQ=255)                                                                                                                                                                                                                                           aCCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGCCTGTAc    <  1:785724/251‑1 (MQ=255)                                                                                                                                                                                                                                           aCCGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGGCAGCGGAGACGGCGCGTATGGGCAGTTAAAATTTGAATCGGGCGGCCACCGCGGGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGCCCGTa     >  1:600909/1‑250 (MQ=255)                                                                                                                                                                                                                                             cGGTGGCGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGCCTGTACTg  <  1:548051/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GAATATGCACAACTTGAGGATGTTGTGAAAAGTTTTAACCGCTGGAATCAGCTCAATTCCAATCTGGAAGAAGCGCAGCTTTTGCTGGACGATCCCGAAATGAAAGAAATGGCGCAAATGGAGATCGAAGAGTCCAAAGCGGAACTTGAACAGGTTGAACAGCATTTACAAATTCTCTTATTGCCGAAAGATCCGAACGACGAATATAACTGTTATCTTGAAATCCGCGCCGGTACCGGTGGTGATGAGGCGGGCATTTTCGCCGGTGATTTATTCCGTATGTACAGCCGTTATGCGGAAACCAAACGTTGGCGTGTTGAAGTGCTCAGTGAAAATGAAAGCGAGCAGGGCGGATTTAAAGAAATTATCGCGTTGGTCAGCGGTGACGGCGTGTATGGTCAGTTAAAATTTGAATCGGGCGGCCACCGCGTGCAGCGAGTGCCGAAAACTGAATCACAAGGGCGTATTCACACCTCTGCCTGTACTG  >  1/1050501‑1050987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑