Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,028,241	T→G	K22T (AAG→ACG)	unknown ←	tRNA ho5U(34) carboxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,028,241	0	T	G	100.0%	193.1 / NA	67	K22T (AAG→ACG)	unknown	tRNA ho5U(34) carboxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/27);  total (40/27)

CGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCTTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATTTCATAAACCTGAGCCAATTCCGTGT  >  1/1027999‑1028470                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      cGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGc                                                                                                                                                                                                                               <  1:117559/251‑1 (MQ=255)   cAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGcc                                                                                                                                                                                                                              <  1:246248/250‑1 (MQ=255)     aTCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATg                                                                                                                                                                                                                           >  1:301195/1‑251 (MQ=255)              aaTGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAgg                                                                                                                                                                                                                   <  1:189679/250‑1 (MQ=255)                      gCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTtggtggtg                                                                                                                                                                                                          >  1:81304/1‑251 (MQ=255)                           tCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGAt                                                                                                                                                                                                     >  1:841725/1‑251 (MQ=255)                                  ggCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGa                                                                                                                                                                                              >  1:618999/1‑251 (MQ=255)                                     cTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATaa                                                                                                                                                                                           >  1:823805/1‑251 (MQ=255)                                                ggCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAg                                                                                                                                                                                       >  1:620114/1‑244 (MQ=255)                                                  cATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAAt                                                                                                                                                                              <  1:647333/251‑1 (MQ=255)                                                     gAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGcc                                                                                                                                                                                                                              >  1:274668/1‑200 (MQ=255)                                                        cTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCa                                                                                                                                                                        >  1:636064/1‑251 (MQ=255)                                                          gTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCAtt                                                                                                                                                                      >  1:70617/1‑251 (MQ=255)                                                                 gtggtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAt                                                                                                                                                               >  1:753683/1‑251 (MQ=255)                                                                 gtggtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAt                                                                                                                                                               >  1:149671/1‑251 (MQ=255)                                                                    gtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAAt                                                                                                                                                                              >  1:636593/1‑233 (MQ=255)                                                                    gtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAtct                                                                                                                                                             >  1:223412/1‑250 (MQ=255)                                                                    gtgCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGAGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAtct                                                                                                                                                             >  1:446111/1‑250 (MQ=255)                                                                      gCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTAt                                                                                                                                                               >  1:588312/1‑246 (MQ=255)                                                                      gCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCg                                                                                                                                                           >  1:448265/1‑250 (MQ=255)                                                                              cGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATaa                                                                                                                                                    <  1:338400/249‑1 (MQ=255)                                                                              cGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAg                                                                                                                                                  <  1:541677/251‑1 (MQ=255)                                                                                ttGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGc                                                                                                                                                 <  1:745497/250‑1 (MQ=255)                                                                                                    gaggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATaa                                                                                                                                                    >  1:764385/1‑227 (MQ=255)                                                                                                    gaggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTggg                                                                                                                             >  1:147656/1‑250 (MQ=255)                                                                                                     aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTggg                                                                                                                             <  1:301006/249‑1 (MQ=255)                                                                                                     aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                                                                                            <  1:273502/250‑1 (MQ=255)                                                                                                     aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                                                                                            <  1:373850/250‑1 (MQ=255)                                                                                                     aggagAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                                                                                            <  1:187044/250‑1 (MQ=255)                                                                                                               cTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAgg                                                                                                                 <  1:540559/251‑1 (MQ=255)                                                                                                               cTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAgg                                                                                                                 <  1:198412/251‑1 (MQ=255)                                                                                                                tttCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGgt                                                                                                                <  1:669408/251‑1 (MQ=255)                                                                                                                     gATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCgg                                                                                                           >  1:281299/1‑251 (MQ=255)                                                                                                                      aTTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGt                                                                                                          <  1:564496/251‑1 (MQ=255)                                                                                                                                   tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTg                                                                                                                                          <  1:638873/206‑1 (MQ=255)                                                                                                                                   tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                                                                                              >  1:653468/1‑250 (MQ=255)                                                                                                                                   tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                                                                                              >  1:686019/1‑250 (MQ=255)                                                                                                                                   tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                                                                                              >  1:361881/1‑250 (MQ=255)                                                                                                                                   tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                                                                                              >  1:896538/1‑250 (MQ=255)                                                                                                                                   tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATc                                                                                              >  1:822055/1‑250 (MQ=255)                                                                                                                                   tttCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCCTCTTTGTACGCCTTTTGGGCTTTGTCAAAgg                                                                                                                 >  1:316029/1‑231 (MQ=255)                                                                                                                                      cAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGt                                                                                          <  1:900804/251‑1 (MQ=255)                                                                                                                                             aTGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGc                                                                                                                                     <  1:762026/201‑1 (MQ=255)                                                                                                                                             aTGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                                                                                            <  1:537151/210‑1 (MQ=255)                                                                                                                                                  ggATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCAt                                                                                                                                                                       >  1:434774/1‑162 (MQ=255)                                                                                                                                                  ggATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGc                                                                                                                            >  1:73379/1‑205 (MQ=255)                                                                                                                                                       ctgcCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGa                                                                         >  1:804935/1‑251 (MQ=255)                                                                                                                                                          ccAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAAc                                                                      <  1:152359/251‑1 (MQ=255)                                                                                                                                                          ccAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAAc                                                                      <  1:24309/251‑1 (MQ=255)                                                                                                                                                              gTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGcc                                                                                                                                    <  1:247399/185‑1 (MQ=255)                                                                                                                                                                   gTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAAt                                                             >  1:391391/1‑251 (MQ=255)                                                                                                                                                                          ttCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACg                                                      >  1:888184/1‑251 (MQ=255)                                                                                                                                                                                gACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGc                                                 >  1:30181/1‑250 (MQ=255)                                                                                                                                                                                          cATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAg                                       >  1:697310/1‑250 (MQ=255)                                                                                                                                                                                           aTTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGcc                                     <  1:519379/251‑1 (MQ=255)                                                                                                                                                                                           aTTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGc                                      >  1:436587/1‑250 (MQ=255)                                                                                                                                                                                                gCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGaa                                >  1:473518/1‑251 (MQ=255)                                                                                                                                                                                                       cGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAAt                                                             >  1:631623/1‑215 (MQ=255)                                                                                                                                                                                                             gggTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGtt                                                   >  1:816894/1‑219 (MQ=255)                                                                                                                                                                                                              ggTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATaa                                                                                                                                                    >  1:302440/1‑121 (MQ=255)                                                                                                                                                                                                               gTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATTTCATAAAcc                  <  1:264087/250‑1 (MQ=255)                                                                                                                                                                                                               gTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATCTCATAAAcc                  <  1:59515/250‑1 (MQ=255)                                                                                                                                                                                                                          ccATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATTTCATAAACCTGAGCCAATTc       <  1:152425/250‑1 (MQ=255)                                                                                                                                                                                                                          ccATTGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATTTCATAAACCTGAGCCAATTc       <  1:577664/250‑1 (MQ=255)                                                                                                                                                                                                                              tGTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATTTCATAAACCTGAGCCAATTCCgtgt  >  1:710874/1‑251 (MQ=255)                                                                                                                                                                                                                               gTTTGAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAAt                                                             >  1:103384/1‑191 (MQ=255)                                                                                                                                                                                                                                   gAGTTGCAGGGGCAGCGTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATTTCGTAAACCTGAGCCAATTc       >  1:309176/1‑241 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      CGCAATCCACATGAATGCCAAGCAAATCATAGGGGCCTTTACGCCACGGCATGAGCTGTTTGAGGTGGTGCACAATACGTTGCCGTTCGCCTTCGGAAAGAGGAGAAACGCTTTCAGATTTGACCGCACTTTTCAAGTCAATGCGGGATGCTGCCAAGTGCGGTAGAAATTCCAAGACTTTTGCCCATTTGGCATAGTCGCCATGGGTTTGTTGCTGCCATTGTTTGAGTTGCAGGGGCAGCTTTTCAAGCCATGGCGAAAGGTTGGTGGTGGCGATTTGTTGATAAAAAGGACGAAAATCGATCATTTTGCTATCTCGCTTATAAAGCTTCTTTGTACGCCTTTTGGGCTTTGTCAAAGGTGTCGGTAATTTCCGCATCCGGTTTTGCCGACAGCAACGAAACCACGAAAATAGCAACGGTTGCCAGTAAAAAGCCCGGAATCATTTCATAAACCTGAGCCAATTCCGTGT  >  1/1027999‑1028470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑