Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	93,281	G→T	A197A (GCC→GCA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	93,281	0	G	T	100.0%	199.6 / NA	67	A197A (GCC→GCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/36);  total (31/36)

ATCATAGCCTGCGGTGTTAATAGCAAGCTCTGTGGCGCCCTGTTGCACATTTTGCAACAACTGGGCGCTGTCGGTAAAAATCGGCATCGACCACACGCGCGAGCCATAACCATACAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTGCACTTTTACCCAAAAAATTAAAGGTAACCACACAATCAATGGTGGGCGCGCCTTGCATGCCGACGTTAACCGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCC  >  1/93068‑93516                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACtcca                                                                                                                                                                                                        >  1:525093/1‑248 (MQ=255) aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACtcca                                                                                                                                                                                                        >  1:171008/1‑248 (MQ=255) aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACtcca                                                                                                                                                                                                        >  1:200497/1‑248 (MQ=255)      aGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGAttt                                                                                                                                                                                                   >  1:289815/1‑251 (MQ=255)            cGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggt                                                                                                                                                                                              <  1:512573/250‑4 (MQ=255)              gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                                                                                                                                            >  1:57148/1‑245 (MQ=255)              gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                                                                                                                                            >  1:52893/1‑245 (MQ=255)              gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                                                                                                                                            >  1:766302/1‑245 (MQ=255)              gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                                                                                                                                            >  1:862783/1‑245 (MQ=255)               tgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCAACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacc                                                                                                                                                                                          <  1:550016/251‑8 (MQ=255)                gtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcac                                                                                                                                                                                           >  1:172226/1‑243 (MQ=255)                       gCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtg                                                                                                                                                                                   <  1:603989/250‑15 (MQ=255)                       gCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtg                                                                                                                                                                                   <  1:109486/250‑15 (MQ=255)                         aaGCTCTGTCGCCCCTTGTTGCCCATTTTGCAACAACTGCGCGCTGTCGTTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                                                                                                                                                <  1:33966/251‑18 (MQ=255)                         aaGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                                                                                                                                                <  1:317029/251‑18 (MQ=255)                           gCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgaccag                                                                                                                                                                              >  1:857482/1‑232 (MQ=255)                             tctgtcgccccttGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctct                                                                                                                                                                                       >  1:119097/13‑230 (MQ=255)                                                    ttGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtg                                                                                                                                                                                   <  1:431488/221‑15 (MQ=255)                                                          caacTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                                                                                                                                                <  1:207025/218‑18 (MQ=255)                                                                    cTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                                                                                                                                      <  1:894178/250‑1 (MQ=255)                                                                    cTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                                                                                                                                      <  1:36114/250‑1 (MQ=255)                                                                    cTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                                                                                                                                      <  1:418612/250‑1 (MQ=255)                                                                     tGTCGGTAAAAATCGGCATCGACCATCCGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATg                                                                                                                                    <  1:883236/251‑1 (MQ=255)                                                                     tGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATg                                                                                                                                    <  1:742735/251‑1 (MQ=255)                                                                        cGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATgcgc                                                                                                                                 <  1:524431/251‑1 (MQ=255)                                                                          gTAAAAATCGGCACCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATgcgc                                                                                                                                 <  1:392872/249‑1 (MQ=255)                                                                               aaTCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                                                                                                                                                <  1:71364/197‑18 (MQ=255)                                                                                   ggCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAAccg                                                                                                                       >  1:299155/1‑248 (MQ=255)                                                                                        cGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAATCTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATgcacggaagtca                                                                                                                                                                                                                 <  1:275405/155‑12 (MQ=255)                                                                                         gACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAAccg                                                                                                                       >  1:130013/1‑242 (MQ=255)                                                                                            catacGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTAcc                                                                                                             <  1:550401/248‑1 (MQ=255)                                                                                             atacGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATgcac                                                                                                                                                                                                                         <  1:852521/140‑4 (MQ=255)                                                                                             atacGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTAccc                                                                                                            <  1:599167/249‑1 (MQ=255)                                                                                                      aGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                                                                                                                                      >  1:248100/1‑216 (MQ=255)                                                                                                      aGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTa                                                                                                   >  1:785298/1‑251 (MQ=255)                                                                                                      aGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTa                                                                                                   >  1:576117/1‑251 (MQ=255)                                                                                                        ccATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGGTGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAggg                                                                                                                             <  1:607539/223‑1 (MQ=255)                                                                                                        ccATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAAccg                                                                                                                       >  1:40064/1‑227 (MQ=255)                                                                                                          aTAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAgg                                                                                               <  1:152848/251‑1 (MQ=255)                                                                                                           tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGGTTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAgg                                                                                               >  1:614305/1‑250 (MQ=255)                                                                                                           tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                                                                                                                                      <  1:207314/211‑1 (MQ=255)                                                                                                           tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGt                                                                                                                            >  1:852650/1‑221 (MQ=255)                                                                                                           tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAgg                                                                                               >  1:627490/1‑250 (MQ=255)                                                                                                           tAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGt                                                                                              >  1:155892/1‑251 (MQ=255)                                                                                                             aCCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTaa                                                                                            >  1:879522/1‑251 (MQ=255)                                                                                                             aCCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTaa                                                                                            >  1:621698/1‑251 (MQ=255)                                                                                                              ccATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTATTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTaa                                                                                            >  1:397328/1‑250 (MQ=255)                                                                                                                    aGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACaacac                                                                                      <  1:545443/250‑1 (MQ=255)                                                                                                                      cgccgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaat                                                                                   <  1:190192/251‑1 (MQ=255)                                                                                                                        ccgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaatca                                                                                 <  1:25156/251‑1 (MQ=255)                                                                                                                         cgcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACAcaatcaa                                                                                <  1:507922/251‑1 (MQ=255)                                                                                                                          gcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATgcacgga                                                                                                                                                                                                                      >  1:893142/1‑110 (MQ=255)                                                                                                                          gcTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAAcaac                                                                                        >  1:325259/1‑239 (MQ=255)                                                                                                                            ttCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtgg                                                                             <  1:396453/251‑1 (MQ=255)                                                                                                                               aaaGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtggtgg                                                                          <  1:684616/251‑1 (MQ=255)                                                                                                                                            tCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCAt                                                             <  1:83562/251‑1 (MQ=255)                                                                                                                                               aCATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGcc                                                          <  1:671204/251‑1 (MQ=255)                                                                                                                                               aCATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGcc                                                          <  1:479769/251‑1 (MQ=255)                                                                                                                                                        aaaCTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAGTGGTGGGCGAGCCCTGCATGCCGACGTTAAc                                                 <  1:18224/251‑1 (MQ=255)                                                                                                                                                              acttTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTggg                                                                         <  1:547628/221‑1 (MQ=255)                                                                                                                                                                        cAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAg                                  >  1:743282/1‑250 (MQ=255)                                                                                                                                                                        cAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAg                                  >  1:553731/1‑250 (MQ=255)                                                                                                                                                                            cgTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtgg                                                                             >  1:173771/2‑203 (MQ=255)                                                                                                                                                                                  gcgcggcgACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAAtggtg                                                                           >  1:350008/1‑199 (MQ=255)                                                                                                                                                                                         gACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAatat                >  1:748436/1‑251 (MQ=255)                                                                                                                                                                                                       aaTGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGcc  <  1:689940/251‑1 (MQ=255)                                                                                                                                                                                                       aaTGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAACCGCACTTTTACCCAAAAAATTAAAGGTAACAACACAATCAATGGTGGGCGAGCCCTGCATGCCGACGTTAACTGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGcc  <  1:56647/251‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            ATCATAGCCTGCGGTGTTAATAGCAAGCTCTGTGGCGCCCTGTTGCACATTTTGCAACAACTGGGCGCTGTCGGTAAAAATCGGCATCGACCACACGCGCGAGCCATAACCATACAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTGCACTTTTACCCAAAAAATTAAAGGTAACCACACAATCAATGGTGGGCGCGCCTTGCATGCCGACGTTAACCGTCCATTTTTTAAGCGCCAGCGGGTTAAATATGCCTGATGTCGGCC  >  1/93068‑93516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑