Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	936,835	A→C	I15L (ATT→CTT)	unknown →	ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	936,835	0	A	C	100.0%	208.5 / NA	69	I15L (ATT→CTT)	unknown	ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/34);  total (35/34)

GGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCGAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTAATTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACAGCAAAAA  >  1/936605‑937077                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ggCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGtt                                                                                                                                                                                                                                >  1:701855/1‑251 (MQ=255)         tGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGc                                                                                                                                                                                                                                              <  1:516676/229‑1 (MQ=255)                         aaaaaTGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTc                                                                                                                                                                                                        <  1:787711/251‑1 (MQ=255)                              tGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTaa                                                                                                                                                                                                   <  1:502696/251‑1 (MQ=255)                                                 aCTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCat                                                                                                                                                                                <  1:697341/251‑1 (MQ=255)                                                  cTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTc                                                                                                                                                                                                                               >  1:726485/1‑203 (MQ=255)                                                  cTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCata                                                                                                                                                                               >  1:136827/1‑251 (MQ=255)                                                                   tttAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTc                                                                                                                                                                                                                               <  1:825871/186‑1 (MQ=255)                                                                                   attcaatATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCat                                                                                                                                                                                <  1:292691/217‑1 (MQ=255)                                                                                                     aaTTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTCCCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAAc                                                                                                                            <  1:366868/251‑1 (MQ=255)                                                                                                                    ttCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAAc                                                                                                             <  1:170960/251‑1 (MQ=255)                                                                                                                      cacaGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACcg                                                                                                           <  1:14650/251‑1 (MQ=255)                                                                                                                                   gTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCtttt                                                                                              >  1:122906/1‑251 (MQ=255)                                                                                                                                   gTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCtttt                                                                                              >  1:18487/1‑251 (MQ=255)                                                                                                                                        aaTTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAAt                                                                                         >  1:290149/1‑251 (MQ=255)                                                                                                                                            aGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACg                                                                                     >  1:35658/1‑251 (MQ=255)                                                                                                                                                taatttaatATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCat                                                                                                                                                                                >  1:76479/1‑156 (MQ=255)                                                                                                                                                  atttaatATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTCCCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGAc                                                                                                                                                                                                             <  1:162521/125‑1 (MQ=255)                                                                                                                                                  atttaatATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTa                                                                               >  1:81686/1‑251 (MQ=255)                                                                                                                                                      aatATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTc                                                                                                                                                                                                                          <  1:717385/108‑1 (MQ=255)                                                                                                                                                      aatATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGAc                                                                                                                                                                                                             <  1:481868/121‑1 (MQ=255)                                                                                                                                                       atatGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTCCCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAAtgtg                                                                          <  1:644016/251‑1 (MQ=255)                                                                                                                                                         atGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTctgc                                                                                                                                     >  1:666029/1‑190 (MQ=255)                                                                                                                                                         atGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAAc                                                                                                                            <  1:679616/199‑1 (MQ=255)                                                                                                                                                           gCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGAt                                                                        <  1:58134/249‑1 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAg                                                                                                                                                                                   >  1:367472/1‑139 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTctgc                                                                                                                                     <  1:29970/185‑1 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAg                                                                   >  1:58171/1‑251 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAg                                                                   >  1:447913/1‑251 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAg                                                                   >  1:72051/1‑251 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAg                                                                   >  1:878459/1‑251 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAg                                                                   >  1:171844/1‑251 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAg                                                                   >  1:873420/1‑251 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAACATTACGCTTATTTGACCTTCGACGATc                                                                                                                                           >  1:861984/1‑179 (MQ=255)                                                                                                                                                              gTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTCTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAAc                                                                                                             <  1:433485/209‑1 (MQ=255)                                                                                                                                                                aTTGATTTAAACTAATTTTGAGAGTAATTATGTCCCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGAc                                                                 <  1:777860/251‑1 (MQ=255)                                                                                                                                                                   gAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTaa                                                                                                                                                                                                   <  1:579182/118‑1 (MQ=255)                                                                                                                                                                   gAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAAc                                                                                                             <  1:477772/204‑1 (MQ=255)                                                                                                                                                                    aGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAAtg                                                                            >  1:271776/1‑236 (MQ=255)                                                                                                                                                                       tAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGc                                                          <  1:705918/251‑1 (MQ=255)                                                                                                                                                                        aaaCTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCa                                                         >  1:450329/1‑251 (MQ=255)                                                                                                                                                                            tAATTTTGAGAGTGAATATGTCCCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTAt                                                     <  1:828610/251‑1 (MQ=255)                                                                                                                                                                            tAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTAt                                                     <  1:457898/251‑1 (MQ=255)                                                                                                                                                                            tAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTAt                                                     <  1:868071/251‑1 (MQ=255)                                                                                                                                                                             aaTTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATg                                                    >  1:146062/1‑251 (MQ=255)                                                                                                                                                                                    agagTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAg                                              <  1:801694/250‑1 (MQ=255)                                                                                                                                                                                        tGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTg                                         <  1:551184/251‑1 (MQ=255)                                                                                                                                                                                          aaTATGTCCCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGtt                                       <  1:292992/251‑1 (MQ=255)                                                                                                                                                                                          aaTATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTc                                                                                                                                                                                                        <  1:654024/90‑1 (MQ=255)                                                                                                                                                                                          aaTATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCctc                                                                                                                                        >  1:103454/1‑154 (MQ=255)                                                                                                                                                                                          aaTATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTc                                                                                                  <  1:8689/192‑1 (MQ=255)                                                                                                                                                                                          aaTATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGtt                                       >  1:100989/1‑251 (MQ=255)                                                                                                                                                                                          aaTATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGtt                                       >  1:386044/1‑251 (MQ=255)                                                                                                                                                                                           atatGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGaa                                            >  1:438569/1‑245 (MQ=255)                                                                                                                                                                                           atatGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGttt                                      >  1:681675/1‑251 (MQ=255)                                                                                                                                                                                            tatGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGCACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTAt                                                     >  1:626353/1‑235 (MQ=255)                                                                                                                                                                                               gTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCa                                                                                                                                                                                 >  1:589986/1‑108 (MQ=255)                                                                                                                                                                                                    tttCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAAtgtg                                                                          <  1:841291/206‑1 (MQ=255)                                                                                                                                                                                                               aTCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAAt                  <  1:172629/251‑1 (MQ=255)                                                                                                                                                                                                                        ttAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACa                                                                                                                  <  1:874374/146‑1 (MQ=255)                                                                                                                                                                                                                        ttAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCttt                                 <  1:777382/227‑1 (MQ=255)                                                                                                                                                                                                                        ttAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACa         >  1:894681/1‑251 (MQ=255)                                                                                                                                                                                                                        ttAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACa         >  1:843670/1‑251 (MQ=255)                                                                                                                                                                                                                        ttAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACa         >  1:843243/1‑251 (MQ=255)                                                                                                                                                                                                                        ttAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACa         >  1:756745/1‑251 (MQ=255)                                                                                                                                                                                                                        ttAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACa         >  1:611238/1‑251 (MQ=255)                                                                                                                                                                                                                               gtcgtcTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGc                                                   <  1:804436/202‑1 (MQ=255)                                                                                                                                                                                                                               gtcgtcTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACAGCaaaaa  >  1:815439/1‑251 (MQ=255)                                                                                                                                                                                                                                 cgtcTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGaa                                            >  1:854587/1‑207 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCGAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTAATTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATATCGCAAAAGATTACGCTTATTTGACCTTCGACGATCCTCTGCTGCTGGAACAAGCCAAACAAGAACCGCGTTTATTCTTTTTAAATCACGCAGGTAATGTGATTTTAGACGAAGTGCAGTATGCACAAGAATTGTTTTCTTTATTAAAGCTTGAAATCGATAAACAGCAAAAA  >  1/936605‑937077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑