Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	925,333	A→G	N403D (AAT→GAT)	unknown →	Cell division protein FtsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	925,333	0	A	G	100.0%	255.2 / NA	83	N403D (AAT→GAT)	unknown	Cell division protein FtsZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/48);  total (35/48)

TGCCTGATATGGTGGATGAAATCCGCGTAACCATTGTTGCAACAGGTATTGGTGAACCCGAAGCGCCGGAGATTCAAATTTCTCCACGTCCACAAGCAGCCCCGAATAATCAACCGATAAATACTCAGTTCGGCGCGCCGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAAATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGGTGTTGTTTAT  >  1/925100‑925564                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        tggtggatgaaatccgcgtaaccattgttgcaacaggtattggtgaacccgaagcgccggagattcaaatttctccacgtctacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGaa                                                                                                                                                                                                                        <  1:426875‑M2/112‑1 (MQ=255)          aaatccgcgtaaccattgttgcaacaggtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGaaa                                                                                                                                                                                                               <  1:831008‑M2/121‑1 (MQ=255)            atccgcgtaaccattgttgcaacaggtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGa                                                                                                                                                                                                             <  1:638741‑M2/123‑1 (MQ=255)                    aaccattgttgcaacaggtattggtaaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATAc                                                                                                                                                                                                          >  1:799515‑M2/121‑246 (MQ=255)                      ccattgttgcaacaggtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTtg                                                                                                                                                                                                   >  1:185111‑M2/119‑251 (MQ=255)                         ttgttgcaacaggtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTtgttc                                                                                                                                                                                                <  1:280311‑M2/136‑1 (MQ=255)                             tgcaacaggtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTtgttcaatg                                                                                                                                                                                            >  1:333424‑M2/112‑251 (MQ=255)                                   aggtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAg                                                                                                                                                                                      >  1:159825‑M2/106‑251 (MQ=255)                                    ggtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAgg                                                                                                                                                                                     >  1:821670‑M2/105‑251 (MQ=255)                                     gtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAGCTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAgg                                                                                                                                                                                     <  1:137347‑M2/147‑1 (MQ=255)                                     gtattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGaaa                                                                                                                                                                                                               <  1:320113‑M2/121‑1 (MQ=255)                                     cctgcctggaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGt                                                                                                                                                                                    <  1:488048‑M2/148‑1 (MQ=255)                                     cacccggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGt                                                                                                                                                                                    <  1:385299‑M2/148‑1 (MQ=255)                                      tattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGt                                                                                                                                                                                    <  1:734885‑M2/148‑1 (MQ=255)                                      tattggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGt                                                                                                                                                                                    <  1:267567‑M2/148‑1 (MQ=255)                                          ggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATa                                                                                                                                                                                                                                      <  1:670500‑M2/98‑1 (MQ=255)                                          ggtgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGt                                                                                                                                                                                    <  1:640445‑M2/148‑1 (MQ=255)                                            tgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGc                                                                                                                                                                             <  1:720898‑M2/155‑1 (MQ=255)                                            tgaacccgaagcgccggagattcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGG‑TGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGc                                                                                                                                                                             <  1:885071‑M2/154‑1 (MQ=255)                                                               attcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAaataat                                                                                                                                                           <  1:245314‑M2/173‑1 (MQ=255)                                                                ttcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTGTGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAatat                                                                                                                                                         <  1:889853‑M2/175‑1 (MQ=255)                                                                ttcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAatat                                                                                                                                                         <  1:762641‑M2/175‑1 (MQ=255)                                                                ttcaaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAatat                                                                                                                                                         <  1:527294‑M2/175‑1 (MQ=255)                                                                  caaatttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAAtatta                                                                                                                                                       <  1:370970‑M2/177‑1 (MQ=255)                                                                      tttctccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGaaa                                                                                                                                                                                                               >  1:603663‑M2/71‑191 (MQ=255)                                                                          tccacgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTgg                                                                                                                                               <  1:400873‑M2/185‑1 (MQ=255)                                                                              cgtccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAAc                                                                                                                                           <  1:530485‑M2/189‑1 (MQ=255)                                                                                tccacaagcagccccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCt                                                                                                                                                    >  1:27066‑M2/61‑240 (MQ=255)                                                                                             ccgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAag                                                                                                                                <  1:247665‑M2/200‑1 (MQ=255)                                                                                              cgaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGaaa                                                                                                                           >  1:343219‑M2/47‑251 (MQ=255)                                                                                               gaataatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAatat                                                                                                                                                         >  1:354845‑M2/46‑220 (MQ=255)                                                                                                   aatcaaccgataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTa                                                                                                                      <  1:34907‑M2/210‑1 (MQ=255)                                                                                                            ataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGc                                                                                                                                                                             >  1:357149‑M2/33‑187 (MQ=255)                                                                                                            ataaatactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAAc                                                                                                             <  1:133613‑M2/219‑1 (MQ=255)                                                                                                                atactcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAAt                                                                                                                                                                        >  1:545596‑M2/29‑188 (MQ=255)                                                                                                                  actcagttcggcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAatat                                                                                                                                                         <  1:715952‑M2/175‑1 (MQ=255)                                                                                                                            gcgcgccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAAc                                                                                              <  1:247515‑M2/234‑1 (MQ=255)                                                                                                                                gccgagaacaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAagag                                                                                                                              <  1:627388‑M2/202‑1 (MQ=255)                                                                                                                                       acaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAg                                                                                  <  1:355180‑M2/246‑1 (MQ=255)                                                                                                                                       acaaaGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAg                                                                                  <  1:212703‑M2/246‑1 (MQ=255)                                                                                                                                            gagaACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTAc                                                                              >  1:250859/1‑250 (MQ=255)                                                                                                                                              gaACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGAt                                                                                                                  >  1:329457/1‑212 (MQ=255)                                                                                                                                                aCAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGataaa                                                                                                                                                               >  1:760228/1‑165 (MQ=255)                                                                                                                                                           aaCTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGACACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTacac                                                              <  1:449650/251‑1 (MQ=255)                                                                                                                                                             cTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCt                                                                                                                                                    <  1:222456/163‑1 (MQ=255)                                                                                                                                                             cTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAAc                                                                                                             >  1:664149/1‑202 (MQ=255)                                                                                                                                                             cTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAg                                                            <  1:496024/251‑1 (MQ=255)                                                                                                                                                              ttATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAgt                                                           >  1:339032/1‑251 (MQ=255)                                                                                                                                                               tATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAatat                                                                                                                                                         <  1:367177/156‑1 (MQ=255)                                                                                                                                                               tATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAgg                                                                                                                         <  1:768273/188‑1 (MQ=255)                                                                                                                                                               tATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTacac                                                              <  1:632951/247‑1 (MQ=255)                                                                                                                                                                aTGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAgtgg                                                         <  1:219822/251‑1 (MQ=255)                                                                                                                                                                   gACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGt                                                                                                                        >  1:495821/1‑185 (MQ=255)                                                                                                                                                                   gACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGAt                                                                                                                  <  1:409239/191‑1 (MQ=255)                                                                                                                                                                     cATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATa                                                    <  1:273215/251‑1 (MQ=255)                                                                                                                                                                      aTAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCgg                                                                           >  1:690656/1‑227 (MQ=255)                                                                                                                                                                      aTAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATaa                                                   >  1:133090/1‑251 (MQ=255)                                                                                                                                                                      aTAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATaa                                                   >  1:208975/1‑251 (MQ=255)                                                                                                                                                                       tAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAAtt                                                                                                                                                                                                     <  1:125210/104‑1 (MQ=255)                                                                                                                                                                         gTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAaataat                                                                                                                                                           >  1:303666/1‑144 (MQ=255)                                                                                                                                                                         gTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATa                                                    <  1:582190/247‑1 (MQ=255)                                                                                                                                                                         gTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGt                                                >  1:183634/1‑251 (MQ=255)                                                                                                                                                                         gTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGt                                                >  1:566940/1‑251 (MQ=255)                                                                                                                                                                         gTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGt                                                >  1:332444/1‑251 (MQ=255)                                                                                                                                                                          tGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAgg                                                 <  1:352325/249‑1 (MQ=255)                                                                                                                                                                                ggATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAg                                                                                  <  1:669710/210‑1 (MQ=255)                                                                                                                                                                                ggATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAgtgg                                                         >  1:166902/1‑235 (MQ=255)                                                                                                                                                                                 gATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACtt                                           <  1:159260/248‑1 (MQ=255)                                                                                                                                                                                          aatTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTacac                                                              >  1:191911/1‑220 (MQ=255)                                                                                                                                                                                              tGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGc                           <  1:865722/251‑1 (MQ=255)                                                                                                                                                                                                    aTAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTgg                                                                                                                                               <  1:599747/129‑1 (MQ=255)                                                                                                                                                                                                           gCAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAagag                                                                                                                              <  1:616980/139‑1 (MQ=255)                                                                                                                                                                                                            cAAAACGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATAcc              <  1:309740/250‑1 (MQ=255)                                                                                                                                                                                                                aCGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTacac                                                              >  1:481087/1‑198 (MQ=255)                                                                                                                                                                                                                aCGTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGgtgt         >  1:640857/1‑251 (MQ=255)                                                                                                                                                                                                                  gTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATactg                                                 >  1:93603/1‑206 (MQ=255)                                                                                                                                                                                                                  gTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGGtgttg       >  1:311034/1‑251 (MQ=255)                                                                                                                                                                                                                  gTACAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGGtgttg       >  1:895606/1‑251 (MQ=255)                                                                                                                                                                                                                     cAACGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTCAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGGTGTTGttt    >  1:154891/1‑251 (MQ=255)                                                                                                                                                                                                                       aCGTAGTAGAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGGTGTTGTTTAt  >  1:708989/1‑251 (MQ=255)                                                                                                                                                                                                                            gtagAGACGATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAAtatta                                                                                                                                                       <  1:443899/97‑1 (MQ=255)                                                                                                                                                                                                                                    gATTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGGTGTTGttt    >  1:305631/1‑236 (MQ=255)                                                                                                                                                                                                                                     aTTTAGATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATgg                                                                                                                                                                      >  1:895003/1‑73 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        TGCCTGATATGGTGGATGAAATCCGCGTAACCATTGTTGCAACAGGTATTGGTGAACCCGAAGCGCCGGAGATTCAAATTTCTCCACGTCCACAAGCAGCCCCGAATAATCAACCGATAAATACTCAGTTCGGCGCGCCGAGAACAAATGCGCCAACTTATGGACATAGTGCACAGGATGCGAATAATTTGTCCAATAACCAGCAAAACGTACAACGTAGTAGAGACGATTTAAATACGCCGATTACCGAACGCTTGAAAGATACTAATTTGTTCAATGTTCAAGGTTTTATGCGTAATGGCGATAAATAATATTATCTTGTGGTAACAGCAAGTTAAGAGAAAGGTTATGATTAAACAAAGAACATTAAAACAAAGTATAAAAGTTACCGGTGTGGGATTACACAGTGGTGATAAGGTTACTTTGGTTTTACGTCCTGCAATGCCGAATACCGGTGTTGTTTAT  >  1/925100‑925564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑