Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	93,161	C→T	V237V (GTG→GTA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	93,161	0	C	T	100.0%	196.3 / NA	70	V237V (GTG→GTA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/22);  total (48/22)

TGCAATCGGATAAACGGTGGTTAAAGTGCGGTCATAATTGCCCACGACCGCGCGTTTGAGCTTGATTTTGTTGGATTCAAGCGCACTAATCTCGACCATTTCTTTGTTTTTTCCTGTAATCAAAATGGCACGGCCACCGACGGCAAAATCATAGCCTGCGGTGTTAATAGCAAGCTCTGTGGCGCCCTGTTGCACATTTTGCAACAACTGGGCGCTGTCGGTAAAAATCGGCATCGACCACACGCGCGAGCCATAACCATACAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTG  >  1/92921‑93399                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               ccgcagcGGATAAACGGTGTTTAAAGTGCGCTAATATTTCCCCACGCCCGCGCGTTTGAGTTTGTTTTTCCTCGGTTCAGGGGCGTTGTTTTCGACCTTTTTTTTGTTGCTTGCGGTCATAAAAACCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGc                                                                                                                                                                                                                                      <  1:653339/245‑1 (MQ=255)         ggtAAACAGTGGTTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATAcg                                                                                                                                                                                                                                             >  1:187236/3‑236 (MQ=255)         ggtAAACAGTGGTTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCa                                                                                                                                                                                                                              >  1:886681/3‑251 (MQ=255)         ggtAAACAGTGGTTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCa                                                                                                                                                                                                                              >  1:543314/3‑251 (MQ=255)         ggtAAACAGTGGTTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCa                                                                                                                                                                                                                              >  1:80681/3‑251 (MQ=255)         ggtAAACAGTGGTTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCa                                                                                                                                                                                                                              >  1:249910/3‑251 (MQ=255)            aaaCAGTGGTTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATag                                                                                                                                                                                                                           >  1:862101/1‑250 (MQ=255)                 gtggtTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAgcg                                                                                                                                                                                                                       >  1:14596/1‑250 (MQ=255)                   ggtTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAAc                                                                                                                                                                                                                                >  1:28546/1‑239 (MQ=255)                   ggtTAAAGTGCGCTCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAgc                                                                                                                                                                                                                        >  1:491700/1‑247 (MQ=255)                              gctCATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAgcg                                                                                                                                                                                                          >  1:752436/3‑250 (MQ=255)                                 cATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGa                                                                                                                                                                                                      >  1:1960/1‑251 (MQ=255)                                 cATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGa                                                                                                                                                                                                      >  1:555352/1‑251 (MQ=255)                                 cATAATTGCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGa                                                                                                                                                                                                      >  1:788551/1‑251 (MQ=255)                                        gCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAAc                                                                                                                                                                                                                                >  1:518439/1‑218 (MQ=255)                                        gCCCACGACCGCGCGCTTGAGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCaa                                                                                                                                                                                               >  1:13718/1‑251 (MQ=255)                                                           aGCTTGATTTTACTCGGTTCAAGGGCGCTGATTTCGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACttt                                                                                                                                                                            >  1:746417/1‑251 (MQ=255)                                                                      actcggttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCaaacg                                                                                                                                                                 >  1:818211/23‑249 (MQ=255)                                                                           gttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCACCATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:762311/18‑251 (MQ=255)                                                                           gttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:762306/18‑251 (MQ=255)                                                                           gttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:112096/18‑251 (MQ=255)                                                                           gttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:83280/18‑251 (MQ=255)                                                                           gttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:97017/18‑251 (MQ=255)                                                                           gttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:151024/18‑251 (MQ=255)                                                                           gttcaagggcgctgatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:123917/18‑251 (MQ=255)                                                                                        gatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACt                                                                                                                                                                       >  1:235910/5‑227 (MQ=255)                                                                                        gatttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACaag                                                                                                                                                >  1:33515/5‑248 (MQ=255)                                                                                          tttcGACCATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAagcgg                                                                                                                                             >  1:588933/3‑251 (MQ=255)                                                                                                ccATTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCa                                                                                                                                       >  1:51729/1‑251 (MQ=255)                                                                                                  aTTTCTTTGTTGCTTGCGGTCATCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAAcc                                                                                                                                                                                                                               >  1:658362/1‑161 (MQ=255)                                                                                                   tttctttgttgcttgcggtcaTCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATg                                                                                                                                    >  1:639873/21‑251 (MQ=255)                                                                                                     tctttgttgcttgcggtcaTCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATgcg                                                                                                                                  >  1:92075/19‑251 (MQ=255)                                                                                                                      caTCAAAATCGCGCGCCCGCCGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGATCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcggcg                                                                                                                                                          >  1:893796/2‑210 (MQ=255)                                                                                                                                     cccgccGATGGCAAAATCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAAc                                                                                                                                                                        <  1:420523/177‑1 (MQ=255)                                                                                                                                                    aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAAcc                                                                                                                                                                                                                               <  1:466154/111‑1 (MQ=255)                                                                                                                                                    aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTAcgg                                                                                                                                                            >  1:383428/1‑178 (MQ=255)                                                                                                                                                    aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATgcgc                                                                                                                                 >  1:684711/1‑205 (MQ=255)                                                                                                                                                    aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACtcca                                                                                   >  1:171008/1‑248 (MQ=255)                                                                                                                                                    aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACtcca                                                                                   >  1:200497/1‑248 (MQ=255)                                                                                                                                                    aTCATAGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACtcca                                                                                   >  1:525093/1‑248 (MQ=255)                                                                                                                                                         aGCCTGCGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGAttt                                                                              >  1:289815/1‑251 (MQ=255)                                                                                                                                                               cGGTGTTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggt                                                                         <  1:512573/250‑4 (MQ=255)                                                                                                                                                                 gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                       >  1:57148/1‑245 (MQ=255)                                                                                                                                                                 gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                       >  1:862783/1‑245 (MQ=255)                                                                                                                                                                 gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                       >  1:766302/1‑245 (MQ=255)                                                                                                                                                                 gtgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtca                                                                       >  1:52893/1‑245 (MQ=255)                                                                                                                                                                  tgtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCAACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacc                                                                     <  1:550016/251‑8 (MQ=255)                                                                                                                                                                   gtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGctgccgtcacagat                                                                                                            >  1:673381/1‑198 (MQ=255)                                                                                                                                                                   gtTAATGGCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcac                                                                      >  1:172226/1‑243 (MQ=255)                                                                                                                                                                          gCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtg                                                              <  1:603989/250‑15 (MQ=255)                                                                                                                                                                          gCAAGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtg                                                              <  1:109486/250‑15 (MQ=255)                                                                                                                                                                            aaGCTCTGTCGCCCCTTGTTGCCCATTTTGCAACAACTGCGCGCTGTCGTTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                           <  1:33966/251‑18 (MQ=255)                                                                                                                                                                            aaGCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                           <  1:317029/251‑18 (MQ=255)                                                                                                                                                                              gCTCTGTCGCCCCTTGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgaccag                                                         >  1:857482/1‑232 (MQ=255)                                                                                                                                                                                tctgtcgccccttGTTGCACATTTTGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctct                                                                  >  1:119097/13‑230 (MQ=255)                                                                                                                                                                                 ctgtcgccccttGTTGCACATTTTGCAACAACGGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACt                                                                                                                                                                       <  1:605663/127‑1 (MQ=255)                                                                                                                                                                                                       ttGCAACAACTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtg                                                              <  1:431488/221‑15 (MQ=255)                                                                                                                                                                                                             caacTGCGCGCTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                           <  1:207025/218‑18 (MQ=255)                                                                                                                                                                                                                ctgcgcgcTGTCGATAAAAATCGGCAGCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACa                                                                                                                                                                                             <  1:652722/81‑1 (MQ=255)                                                                                                                                                                                                                       cTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                 <  1:894178/250‑1 (MQ=255)                                                                                                                                                                                                                       cTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                 <  1:418612/250‑1 (MQ=255)                                                                                                                                                                                                                       cTGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATaa                 <  1:36114/250‑1 (MQ=255)                                                                                                                                                                                                                        tGTCGGTAAAAATCGGCATCGACCATCCGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATg               <  1:883236/251‑1 (MQ=255)                                                                                                                                                                                                                        tGTCGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATg               <  1:742735/251‑1 (MQ=255)                                                                                                                                                                                                                           cGGTAAAAATCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATgcgc            <  1:524431/251‑1 (MQ=255)                                                                                                                                                                                                                             gTAAAAATCGGCACCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATgcgc            <  1:392872/249‑1 (MQ=255)                                                                                                                                                                                                                                  aaTCGGCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTcggtcacctcttgtgacc                                                           <  1:71364/197‑18 (MQ=255)                                                                                                                                                                                                                                      ggCATCGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAAccg  >  1:299155/1‑248 (MQ=255)                                                                                                                                                                                                                                           cGACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAATCTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATgcacggaagtca                                                                                            <  1:275405/155‑12 (MQ=255)                                                                                                                                                                                                                                            gACCATACGCGCGAGCCATAACCATAGAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAACGTACGGCGCGGCGACAAGCGGCGGGCAATGCGCTGCTCTGCTGCCGTCACAGATTGATGCACGGAAGTCAGCCACTCCAGATTTTCGGTCACCTCTTGTGACCAGTCCGGCAGAAATGCCCAATCGGTTGAACGCGAGCCTATAATGCGCAGGGTAAccg  >  1:130013/1‑242 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               TGCAATCGGATAAACGGTGGTTAAAGTGCGGTCATAATTGCCCACGACCGCGCGTTTGAGCTTGATTTTGTTGGATTCAAGCGCACTAATCTCGACCATTTCTTTGTTTTTTCCTGTAATCAAAATGGCACGGCCACCGACGGCAAAATCATAGCCTGCGGTGTTAATAGCAAGCTCTGTGGCGCCCTGTTGCACATTTTGCAACAACTGGGCGCTGTCGGTAAAAATCGGCATCGACCACACGCGCGAGCCATAACCATACAGCGCCGCTTCAAAGCGCTGACGCTCAACATCGCCGAAACTTACTTTAAACTCAAATGTACGGCGCGGCGACAGGCGGCGGGCAATGCGCTGCTCTGCGGCAGTGACGGATTGATGTACAGAGGTCAGCCACTGCAGGTTTTCTGTGACATCCTCGCTCCAATCCGGCATAAACGACCAATCAGTTGAGCGCGAGCCTATAATGCGCAGGGTAACTG  >  1/92921‑93399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑