Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,586,1420TC67.7% 28.3 / 18.6 34intergenic (‑191/+92)unknown/unknownSSU rRNA ## 16S rRNA, small subunit ribosomal RNA/Glutamate racemase (EC 5.1.1.3)
Reads supporting (aligned to +/- strand):  ref base T (4/7);  new base C (17/6);  total (21/13)
Fisher's exact test for biased strand distribution p-value = 5.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGAGCCATGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTGAGATCATTTTTGTGCAAAACACTTGATTATTTGTTTGATTTTTTGACCG  >  1/1585923‑1586377
                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
tGAGCCATGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAGCCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGCTTTTATcc                                                                                                                                                                                                              <  1:835148/251‑1 (MQ=14)
       tGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAgg                                                                                                                                                                                                                       <  1:722368/235‑1 (MQ=31)
        gATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGtatta                                                                                                                                                                                                                           <  1:94891/230‑1 (MQ=31)
                                        cTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGcc                                                                                                                                                                                                                                    <  1:751933/189‑1 (MQ=32)
                                                  gACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGcc                                                                                                                                                                                                                                    >  1:358640/1‑179 (MQ=32)
                                                  gACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTAc                                                                                                                                                                                                          >  1:108275/1‑205 (MQ=31)
                                                  gACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCt                                                                                                                                                                                                        <  1:344076/207‑1 (MQ=31)
                                                    cTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCtt                                                                                                                                                          >  1:469808/1‑251 (MQ=39)
                                                                attatAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGc                                                                                                                                                                                                                      <  1:231757/179‑1 (MQ=32)
                                                                     aaataatataaataaACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAAttt                                                                                                                                          >  1:884834/1‑250 (MQ=255)
                                                                                       aaTCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTAt                                                                                                                        <  1:584858/250‑1 (MQ=255)
                                                                                           ttCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTAttttt                                                                                                                                                    <  1:269801/218‑1 (MQ=255)
                                                                                                   ccTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTAcc                                                                                                                                                                                                         <  1:150206/157‑1 (MQ=32)
                                                                                                   ccTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTAcc                                                                                                                                                                                                         >  1:258329/1‑157 (MQ=32)
                                                                                                      aTTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACg                                                                                                        >  1:853540/1‑251 (MQ=255)
                                                                                                                aTCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAg                                                                                                                                                                                                                        >  1:511436/1‑129 (MQ=33)
                                                                                                                 tCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCaa                                                                                             >  1:854061/1‑251 (MQ=255)
                                                                                                                          cATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTAC‑TTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTTTTTTTTGTCAAATTTTTTTTCAAACCTTAATATTGACATTTAATAAACGCAGCGTTTTCAATCCtttct                                                                                    >  1:899665/1‑246 (MQ=17)
                                                                                                                                              gTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCaaa                                                                                                                                  <  1:406529/185‑1 (MQ=255)
                                                                                                                                              gTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGAc                                                                                  <  1:310525/233‑1 (MQ=255)
                                                                                                                                              gTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAAt                                                                        >  1:453314/1‑243 (MQ=255)
                                                                                                                                              gTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTaaa                                                                >  1:753411/1‑251 (MQ=255)
                                                                                                                                                       caGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTc                                                        >  1:855484/1‑250 (MQ=255)
                                                                                                                                                         gTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAAc                                                                                                         <  1:877773/199‑1 (MQ=255)
                                                                                                                                                                                 aCAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGAc                                                                                  >  1:819327/1‑198 (MQ=255)
                                                                                                                                                                                                  gTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCt                                                                                                                >  1:269226/1‑151 (MQ=255)
                                                                                                                                                                                                  gTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAGATCCTAAAg                                                                                    >  1:454733/1‑179 (MQ=255)
                                                                                                                                                                                                  gTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTg                                                   >  1:350211/1‑212 (MQ=255)
                                                                                                                                                                                                  gTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTGAGATCAtt                                           <  1:155098/220‑1 (MQ=255)
                                                                                                                                                                                                  gTCTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTGAGATCATTTTTGTGCAAAACACTTGATTATTTGTTTGAt            >  1:409307/1‑251 (MQ=255)
                                                                                                                                                                                                    cTATAAAACCCTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTGAGATCATTTTTGTGCAAAACACTTGATTATTTGTTTGAttt          >  1:316294/1‑251 (MQ=255)
                                                                                                                                                                                                            cccTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTGAGATCATTTTTGTGCAAAACACTTGATTATTTGTTTGATTTTTTGACCg  >  1:409900/1‑251 (MQ=255)
                                                                                                                                                                                                              cTACAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCaa                                                                                             >  1:97732/1‑158 (MQ=255)
                                                                                                                                                                                                                aCAACGGCGCGCCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTGAGATCATTTTTGTGc                                    >  1:728484/1‑213 (MQ=255)
                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
TGAGCCATGATCAAACTCTTCAATTCAAAAAGCTTAATCCCTCAATAGCTGACTTAATTAATCTATTATAAATAATATAAATAAACGAATCTTCCGGCACCTATTAAGTTCAATCTTAAAATCATTTAAAACGAATCTATAAGTGCCCACACAGTTTGTATGATTGATTGTTAAAGAACAAAAAACGACGCGCTGTCTATAAAACCCTACAACGGCGCGTCGTTGTGCCCGCGTATTATAGGCTTTTATCCTTACCTTGCAAGTACTTTTTTACAAAAAATTTAAAAATCTTTTTGATTGCTTATTTTTTGTCAAATTTCTCTCAAAACCTCACTATCGACATCTAATAAACGCAGCGTTTCAAATCCTAAAGACTGAATTAAATTTGGTAAACCGTCTTCCGGTGAGATCATTTTTGTGCAAAACACTTGATTATTTGTTTGATTTTTTGACCG  >  1/1585923‑1586377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑