Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	1	1,568,439	+AATAA	coding (31/555 nt) coding (410/420 nt)	unknown ← unknown ←	Phosphoglycerate transport system transcriptional regulatory protein PgtA Phosphoglycerate transport system transcriptional regulatory protein PgtA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	1	= 1568439	0 (0.000)	23 (0.270) +AATAA	19/420	2.8	100%	coding (31/555 nt) coding (410/420 nt)	unknown unknown	Phosphoglycerate transport system transcriptional regulatory protein PgtA Phosphoglycerate transport system transcriptional regulatory protein PgtA
	?	1	1568440 =	0 (0.000)					coding (30/555 nt) coding (409/420 nt)	unknown unknown	Phosphoglycerate transport system transcriptional regulatory protein PgtA Phosphoglycerate transport system transcriptional regulatory protein PgtA

ATCAGTGATATGTTTACGTAACGGCAGCATTTCAATTTGAGTATAAATAAAAAAATAATAAAACTCGGCACTTATCTGCCGACAGCGAATTAATTCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/1568197‑1568439 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTCGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCG  >  1/1568440‑1568689                                                                                                                                                                                                                                                    |||||                                                                                                                                                                                                                                                           ATCAGTGATATGTTTACGTAACGGCAGCATTTCAATTTGAGTATAAATAAAAAAATAATAAAACTCGGCACTTATCTGCCGACAGCGAATTAATTCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATAT                                                                                                                                                                                                                                                         <  1:789096/251‑1           TGTTTACGTAACGGCAGCATTTCAATTTGAGTATAAATAAAAAAATAATAAAACTCGGCACTTATCTGCCGACAGCGAATTAATTCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAA                                                                                                                                                                                                                                                            >  1:763249/1‑238                                                                       CTTATCTGCCGACAGCGAATTAATTCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAG                                                                                                                                                                                   >  1:814587/1‑251                                                                         TATCTGCCGACAGCGAATTAATTCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGA                                                                                                                                                                                                    <  1:88137/232‑1                                                                                                    AAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTG                                                                                                                                                       >  1:253250/1‑250                                                                                                      GTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAA                                                                                                                                                                                                                                                            >  1:731207/1‑147                                                                                                         CTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATC                                                                                                                                                 <  1:705590/251‑1                                                                                                                                  GAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAAC                                                                                                                                <  1:149387/243‑1                                                                                                                                           CTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGG                                                                                                                                                                                                                 >  1:277990/1‑153                                                                                                                                                     TGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGC                                                                                                                                                                                     >  1:740536/1‑171                                                                                                                                                      GTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGT                                                                                                                                                                                                                                      >  1:193183/1‑121                                                                                                                                                                            TTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAA                                                                              >  1:437030/1‑251                                                                                                                                                                              GTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATA                                                                                                                                                                                                                                                    >  1:587179/1‑83                                                                                                                                                                                         GGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGC                                                                  <  1:152808/250‑1                                                                                                                                                                                            AGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATAT                                                                                                                                                                                                                                                         >  1:781965/1‑64                                                                                                                                                                                             GATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATC                                                                                                                                                 <  1:543309/167‑1                                                                                                                                                                                             GATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGT                                                                                                                                                                             >  1:299606/1‑139                                                                                                                                                                                              ATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAA                                                            >  1:816438/1‑251                                                                                                                                                                                                  TGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTT                                                                                             >  1:1020/1‑214                                                                                                                                                                                                                                      TCTTTAATTAGCTCAATAATATTAATATTATCATTTTGTGTACATT                                                                                                                                                                                                                                 <  1:536106/46‑1                                                                                                                                                                                                                                                GCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCAC           >  1:804040/1‑250                                                                                                                                                                                                                                                GCTCAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTG                                                                                               >  1:501848/1‑166                                                                                                                                                                                                                                                   CAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTT       >  1:265934/1‑251                                                                                                                                                                                                                                                   CAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAA                                                      >  1:321912/1‑204                                                                                                                                                                                                                                                   CAATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGAT                                                                                                                        >  1:644087/1‑138                                                                                                                                                                                                                                                     ATAATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGC                      <  1:549702/234‑1                                                                                                                                                                                                                                                        ATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCG  >  1:4846/1‑251                                                                                                                                                                                                                                                        ATATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTAGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAA                                                                                                  >  1:736996/1‑155                                                                                                                                                                                                                                                    |||||                                                                                                                                                                                                                                                           ATCAGTGATATGTTTACGTAACGGCAGCATTTCAATTTGAGTATAAATAAAAAAATAATAAAACTCGGCACTTATCTGCCGACAGCGAATTAATTCGTTAAGTTCTATATCACTAAGCAAAATTAAACGGAATTGACTCTCGTCATGATGTAAAATCTGAATTAAATATTGTTGTTCTTCAACAGGAAGATAATGCACATTCTTAATAATTAAAGTCCCTGATTTTGTTTCTTTAATTAGCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  1/1568197‑1568439 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TATTAATATTATCATTTTGTGTACATTCATGAAAAACCATCGGTAGTGTTTTTCGATCGCTTAATTTATGCAGATAAGTCGCCGCTAAATGACGACCGGTGCCAATCTCTCCCCAAATAAAAACCGGTAGATTCGAATTGGCCAGCTTCTGTAATTGTTTTTTTAACTCGTTAATCCAAACACTGCTGCCAATAAAAACGTCATTAATTTTTTCCATTTGCCATTGGCGCTTTTCAATCACCTTTTTCCG  >  1/1568440‑1568689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.