Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,851,516	(T)5→6	coding (533/594 nt)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,851,511	1	.	T	100.0%	30.7 / NA	12	F180I (TTT→ATT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (3/9);  total (3/9)

ATAAAAGTCAAAATAAAATAAAATAAAATAAATAATTTAGAATAAAATTTATTTAATAGCATAATATTTATTAATTACCTTTTCAACATAGTCTTTATCAAATTTGATACCTAATTTTTGATAAACATCCTGTAATATAGAAACATCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAA‑TTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCCCTTCCTTAAGCAGGTAATTAGTAACTTTTACAACATCATCTACATCTATAAAATTTCTATGTGCTTTACCAAAAATAGAAAAACATTCTCTATTCTTTAT  >  1/1851309‑1851691                                                                                                                                                                                                            |                                                                                                                                                                                     ataaAAGTCAAAATAAAATAAAATAAAATAAATAATTTAGAATAAAATTTATTTAATAGCATAATATTTATTAATTACCTTTTCAACATAGTCTTTATCAAATTTGATACCTAATTTTTGATAAACATCCTGTAATATAGAAACATCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAg                                                                                                                                       <  1:252371/251‑1 (MQ=255)    aaaGTCAAAATAAAATAAAATAAAATAAATAATTTAGAATAAAATTTATTTAATAGCATAATATTTATTAATTACCTTTTCAACATAGTCTTTATCAAATTTGATACCTAATTTTTGATAAACATCCTGTAATATAGAAACATCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATg                                                                                                                                    <  1:143126/251‑1 (MQ=255)                                                                                      aCATAGTCTTTATCAAATTTGATACCTAATTTTTGATAAACATCCTGTAATATAGAAACATCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCCCTTCCTTAAGCAGGTAATTAGTAACTTTTACAAcat                                                                  <  1:14889/235‑1 (MQ=255)                                                                                                                                    gTAATATAGAAACATCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGa                                                                                                                                      <  1:357345/121‑1 (MQ=255)                                                                                                                                      aaTATAGAAACATCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCCCTTCCTTAAGCAGGTAATTAGTAACTTTTACAACATCATCTACATCTATAAAATTTCTATGTGCTTTACCAAAAATAGAAAAACATTCTCTATTCtttat  <  1:14460/251‑1 (MQ=255)                                                                                                                                                  tCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGc                                                                                                                                   >  1:515161/1‑110 (MQ=255)                                                                                                                                                   cTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCccttcct                                                                                                <  1:4537/144‑1 (MQ=255)                                                                                                                                                   cTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCCCTTCCTTAAGCAGGTAATTAGTAACTTTTACAACATCATCTACATCTATAAAATTTCTATGTGc                                      <  1:678142/202‑1 (MQ=255)                                                                                                                                                     ataAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCCCTTCCTTAAGCAGGTAAtt                                                                                   <  1:486764/155‑1 (MQ=255)                                                                                                                                                                    ccTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCt                                                                                                                                  >  1:670128/1‑93 (MQ=255)                                                                                                                                                                                   ttATAAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCCCTTCCTTAAGCAGGTAATTAGTAACTTTTACAACATcatctacatc                                                        <  1:454255/152‑1 (MQ=255)                                                                                                                                                                                     ataAAAAGCTCTTTGGTTAGAAATTTTTTCAAGATAAAGAATAAGTTCATACATTGACGTatga                                                                                                                                              >  1:535082/1‑64 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                     ATAAAAGTCAAAATAAAATAAAATAAAATAAATAATTTAGAATAAAATTTATTTAATAGCATAATATTTATTAATTACCTTTTCAACATAGTCTTTATCAAATTTGATACCTAATTTTTGATAAACATCCTGTAATATAGAAACATCTATAAAATATCTACTTCCTTTATTTTCCACATTATAAAAAGCTCTTTGGTTAGAAA‑TTTTTCAAGATAAAGAATAAGTTCATACATTGACGTATGATGTGTAGATGCTAAATTAACAATAGAATTAATAAACAGCCCTTCCTTAAGCAGGTAATTAGTAACTTTTACAACATCATCTACATCTATAAAATTTCTATGTGCTTTACCAAAAATAGAAAAACATTCTCTATTCTTTAT  >  1/1851309‑1851691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑