| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 103,593 | Δ1 bp | coding (617/684 nt) | unknown → | Transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 103,593 | 0 | G | . | 93.1% | 85.3 / 2.9 | 29 | coding (617/684 nt) | unknown | Transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (18/9); minor base C (1/1); total (19/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA > 1/103372‑103719 | gcgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatca > 1:517750/1‑251 (MQ=21) cgcTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAatcat < 1:66536/251‑1 (MQ=21) cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagc < 1:557006/225‑3 (MQ=21) cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg > 1:606212/1‑251 (MQ=255) cTATTGCGCCGTTTTTTGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCg > 1:408613/1‑251 (MQ=255) tttGGGCGGACGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac < 1:651874/249‑1 (MQ=21) aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa < 1:252470/250‑1 (MQ=21) aCGGCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaa < 1:855946/250‑1 (MQ=21) gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAGGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa > 1:450671/1‑250 (MQ=21) gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa > 1:745705/1‑250 (MQ=21) gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa > 1:637457/1‑250 (MQ=21) gCGTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGaaaaa > 1:106359/1‑250 (MQ=21) gTATCAATGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGca > 1:13074/1‑236 (MQ=21) aaTGCGCCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAttt < 1:822715/251‑1 (MQ=18) gcgcCCAACAATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTaaa > 1:540019/1‑251 (MQ=18) cccaacaATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAACCCaagct > 1:207977/5‑184 (MQ=18) caATCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggcttt > 1:881059‑M1/1‑244 (MQ=255) aTCGACGACGGCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaa > 1:684641‑M1/1‑242 (MQ=255) ggCGATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgtttttt > 1:410584‑M1/1‑233 (MQ=255) gATCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac > 1:729910/1‑207 (MQ=18) tCAGATATTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGcac < 1:597917/205‑1 (MQ=18) gaaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag > 1:346617‑M1/1‑204 (MQ=255) aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagt < 1:645803‑M1/251‑49 (MQ=255) aaagaGATTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaag < 1:697688‑M1/250‑48 (MQ=255) gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTAtt > 1:755770/1‑193 (MQ=17) gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta > 1:285583‑M1/1‑198 (MQ=255) gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta > 1:262715‑M1/1‑198 (MQ=255) gaTTAAAGGCGATAAAATCTATTTGGTACAGAACGGCACCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggcta > 1:663178‑M1/1‑198 (MQ=255) aaaGGCGATAAAATCTATTTGGTACAGAACGGCGCCAGCGTATGGGTTAAGCGCGTTAAAATCAATTGGGACGGGTTAGAGCTCATTTCAGACAATAAAGAAGAATATCCACCAATCCAACTAA‑CCCAAGCTGAAGCCGAAAACCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACgggctttaaacgttttttaaacgcctttttgtttttctacaattaagtggctaaaac < 1:489533‑M1/251‑58 (MQ=255) | GCGCTATTGCGCGGTTTTCTGGGCGGACGGCGTATCAATGCGCCCCACAATCGACGACGGCGATCAGATGTTAGTGGATTTATCAAAGAAAGAGATTAAAGGCGACAAAATCTATCTGGTGCAGAACGGGGCCAGCGTATGGGTTAAGCGGGTTAAAATCAACTGGGACGGGTTAGAGCTCATTTCAGACAATAGAGAGGAATACCCACCCATTCAATTAAGCCAAAGC‑GAAGCCGAAACCCTGCAAATCATCGGGCAAGTCGTGCACATCGGGAAAAACCTTATTTAAACCGGCTTTAAACGATTTTTAAACGCTTTTTAAAGCCTCGTTTAAATTTCTTAAATTAA > 1/103372‑103719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |