| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 779,881 | 2 bp→GC | intergenic (+45008/+41951) | unknown → / ← unknown | tRNA‑Asn‑GTT/tRNA‑Thr‑GGT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 779,881 | 0 | A | G | 100.0% | 84.5 / NA | 25 | intergenic (+45008/+41952) | unknown/unknown | tRNA‑Asn‑GTT/tRNA‑Thr‑GGT |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/9); total (16/9) | |||||||||||
| * | 1 | 779,882 | 0 | T | C | 96.0% | 73.4 / ‑3.8 | 25 | intergenic (+45009/+41951) | unknown/unknown | tRNA‑Asn‑GTT/tRNA‑Thr‑GGT |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (15/9); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
GCGTTAACTCAATCTTAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACC > 1/779751‑780071 || ttgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAAt > 1:97588/26‑251 (MQ=255)ttgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAAt > 1:71488/26‑251 (MQ=255) tgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATc < 1:1692/227‑1 (MQ=255) tgttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATc < 1:737046/227‑1 (MQ=255) gttacgcattgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATca > 1:264620/24‑250 (MQ=255) ttgcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcaggg > 1:117857/16‑242 (MQ=255) gcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcagggtgcttgg > 1:701250/14‑240 (MQ=255) gcattacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATcaggg < 1:460821/231‑5 (MQ=255) ttacagtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCg > 1:485452/11‑251 (MQ=255) gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg > 1:418658/6‑249 (MQ=255) gtcttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCagg > 1:217860/6‑249 (MQ=255) tctttaaGGAGACCCTTTATTATGAAAGTCATATCCGTATTAAGTCAGAAAGGCGGTAGCGGTAAAAGCACGCTTTCTATCAATATTGCCCGCAGCCTGCAACTTAAAGGTTTTGATGTCGCATTAATTGATACCGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTAGTCGCCAAg > 1:2884/6‑250 (MQ=255) cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg > 1:585774/4‑251 (MQ=255) cttaaaGGAGGCCCTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCg > 1:658698/4‑251 (MQ=255) cccTTTATCATGAAAGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAAt > 1:544201/1‑251 (MQ=255) aaGTTATATCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggag > 1:310955/1‑247 (MQ=255) agttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagc < 1:2741/245‑4 (MQ=255) gttatatCTGTATTAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctc > 1:583782/4‑245 (MQ=255) tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgc < 1:533598/243‑11 (MQ=255) tatctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccg > 1:554259/9‑241 (MQ=255) tctgtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATggagctccccgcat < 1:278310/245‑13 (MQ=255) gtattAAGCCAGAAAGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGc < 1:152677/175‑1 (MQ=255) ccagaaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCa > 1:663622/5‑251 (MQ=255) aaaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAc < 1:432086/251‑1 (MQ=255) aaGGTGGCAGTGGTAAAAGCACGCTTTCTATCAATATTGCCCGCTGCCTGCAACTTAAAGGCTTTGATGTCGCATTAATTGATACTGACCCACAAGCGTCCGCGCGTGAGTGGAATGCGCTCGCCGGTGATGACTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTTTCCGAAAAAGAAATCAGGGTGCTTGGTCGCCAGGCGGATTTTTTAATTATTGATGGAGCTCCCCGCATTGAAAAAGCCATGAcc < 1:708465/251‑1 (MQ=255) || GCGTTAACTCAATCTTAACCGTTTCAAAGGAGACCCTTTATTATGAAAGTCATATCCGTTTTAAGTCAAAAAGGTGGTAGCGGTAAAAGTACGCTCTCAATTAATATCGCTCGTTGTTTGCAGCTCAAAGATTTTGAAGTGGCATTAATTGACACTGACCCGCAAGCGTCCGCGCGTGAATGGAATGCGCTTGCCGGTGATGATTTTTTCCCGGTGTTTGCTTGTGATAAAGGGCTCTCCGAAAAAGAAATCCGTGCGCTTGGTCGCCAAGCGGATTTTTTAATCATTGATGGTGCGCCACGTATTGAAAAAGCCATGACC > 1/779751‑780071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |